rev: January 12, 2004

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ANTIBODIES  

(anti-Human and others as indicated)

RDI Divison of researchd Industries Intl  offers a wide line of  antibodies. Since no one antibody works best for all applications (neutralization, blotting, histochemistry, ELISA, etc), we offer many different types of antibodies to help solve this problem. Please inquire for other applications or types of antibodies not listed below.

PSA (see also bulk clones for Elisa use)
Mab to Prostate Specific Antigen (PSA)

cat#RDI-PRO10815       $375.00/vial

Clone                                          ER-PR8

Category                                     Mouse monoclonal, hybridoma

Reagent                                      Ascites

Ig Class                                      IgG1

Immunogen                                Isolated from human seminal plasma

Specificity/Clinical Application The presence of prostate specific antigen is highly specific for demonstration of the prostatic origin of a malignancy. Mab 222MPA has been shown to be a valuable reagent for the identifica-tion of primary and metastatic prostatic carci-noma. The antibody reacts with a 34 kDa protein in benign and malignant prostatic epithelium.

Recommended

Positive Control                         Prostatic benign hyperplasia

Application                               · Immunoblotting · Immunohistochemistry · Cell preparations

                                                · suitable for frozen and paraffin sections*

                                                * no pretreatment required

Antigen Recognized in Species

(tested so far)                          Human

Working Dilution                     1:10

Dilution Buffer                        PBS with BSA, optimal dilution should be tested by serial dilution

Incubation Time                     1 h at room temperature

Storage At                             4°C (undiluted) or at -20°C (aliquots)

Volume                                 1 ml

References:

Gallee MPW et al.: Variation of prostate specific antigen expression in different tumor growth patterns present in prostatectomy specimens.

Urological Research 18, 181-187 (1990).

For In Vitro Research Use Only-Not For Use in or On Humans or Animals-Not for use in Diagnsotics

DATA SHEET :Mouse anti-Human Prostate Specific Antigen (PSA) (frozen & Paraffin Sections)

cat# RDI-PSAabm-A4   $375.00/vial

Preparation: Lyophilized antibody purified by Protein G affinity chromatography diluted in 1% w/v BSA in PBS with 15mM sodium azide. Reconstitute with 1ml of sterile distilled water.

Clone: PSA 28/A4

Antigen: seminal fluid preparation

Isotype: mIgG1k

Specificity: Human prostate specific antigen (this antibody reacts with prostatic secretory and ductal epithelium in both normal and neoplastic tissue and can be useful in the identification of metastitic carninomas of prostatic origin)

USE: Effective on paraffin wax embedded tissue and on frozen sections.

Protocol: Immunohistochemistry:Typical working dilution 1:50 - 1:100. 60 Minutes incubation at 25'C. Standard ABC technique.

Positive controls: normal prostate gland or known prostate carcinoma

Staining pattern: cytoplasmic

Storage and stability: Store unopened lyophilized antibody at 4'C. Under these conditions, there is no significant loss in product performance up to the expiry date indicated on the vial label. The reconstituted antibody is stable for at least two months when   stored at 4'C. For long term storage, aliquot in non frost freze freezer, Avoid repeated freeze thaw cycles. Prepare fresh working dilutions daily. Stricly for in vitro research use only-Not for use in or on humans oranimals-not for use in diagnostics

Mouse anti-Prostate Specific Antigen (Cocktail) for WB & histo

cat# RDI-PSA85abm      $375.00/100ug

       RDI-PSA85abm-1  $500.00/200ug

Presentation: Protein G purified antibody in 10mM PBS with 0.2% BSA and 15mM NaN3. (Bulk available without BSA/Azide upon request) concentration 200ug/ml

Immunogen: purified human PSA

Isotype: mIgG1 (both)

Clones: ER-PR8 & PA05

Reactivity: human(others not tested)

Uses: -Western blot: 1ug/ml 2 hr rt -Western blot: 1ug/ml 2 hr rt (recommend clone ER-PR8

alone for best westernblot reactivity cat#RDI-PSAR8abm   $438.00/200ug

-histochemistry (frozen and paraffin sections) approx 1-2ug/ml 30-60 minutes RT

-for formalin fixed sections, requires pretreatment in boiling 10mM citrate bufer pH6.0 for 10-20 minutes followed by cooling at RT for 20 min.

Positive Contrl: prostate or prostate carcinoma


Localization: Cytoplasmic

Background: Recognizes a single protein of 33-34kDa, identified as PSA. This "cocktail" is composed of two clones whose epitopes are not precisely mapped, but experimental data suggets that they are different. This cocktail is highly specific to PSA and stains prostatic secretory and ductal epithelium in both normal and neoplastic tissues. PSA is a chymotrypsin-like serine protease (kallikrein family) exclusively produced by the prostate pithelium, and abundant in seninal fluid. PSA can be detected in the sera of patients with prostatic carcinoma. It is predominantly complexed to a liver derived serine protease inhibitor, alpha-1- antichymotrypsin. A higher proportion of serum PSA is complexed to ACT in prostate cancer than in benign prostate hyperplasia.

Storage: 2-8 deg c

For Research Use Only

RDI Divison of researchd Industries Intl

San Jose, 95123 CA Snell ave 658

USA

or (800) 370-2222

EMAIL:margaret@cellular-research.com

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