rev: January 12, 2004
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ANTIBODIES
(anti-Human and others as indicated)
RDI Divison of researchd Industries Intl offers a wide line of antibodies. Since no one antibody works best for all applications (neutralization, blotting, histochemistry, ELISA, etc), we offer many different types of antibodies to help solve this problem. Please inquire for other applications or types of antibodies not listed below.
cat#RDI-PRO10815 $375.00/vial
Clone
ER-PR8
Category
Mouse monoclonal,
hybridoma
Reagent
Ascites
Ig Class
IgG1
Immunogen
Isolated from human seminal
plasma
Specificity/Clinical Application The presence of prostate specific antigen
is highly specific for demonstration of the prostatic origin of a malignancy.
Mab 222MPA has been shown to be a valuable reagent for the identifica-tion
of primary and metastatic prostatic carci-noma. The antibody reacts with
a 34 kDa protein in benign and malignant prostatic epithelium.
Recommended
Positive Control
Prostatic benign hyperplasia
Application · Immunoblotting · Immunohistochemistry · Cell preparations
· suitable for frozen and paraffin sections*
* no pretreatment required
Antigen Recognized in Species
(tested so far)
Human
Working Dilution
1:10
Dilution Buffer
PBS with BSA, optimal dilution should be tested by serial
dilution
Incubation Time
1 h at room temperature
Storage At
4°C (undiluted) or at -20°C
(aliquots)
Volume
1 ml
References:
Gallee MPW et al.: Variation of prostate specific antigen expression in different tumor growth patterns present in prostatectomy specimens.
Urological Research 18, 181-187 (1990).
For In Vitro Research Use Only-Not For Use in or On Humans or Animals-Not for use in Diagnsotics
Specificity: Human prostate specific antigen (this antibody reacts with prostatic
secretory and ductal epithelium in both normal and neoplastic tissue and
can be useful in the identification of metastitic carninomas of prostatic
origin)
USE: Effective on paraffin wax embedded tissue and on frozen sections.
Protocol: Immunohistochemistry:Typical working dilution 1:50 - 1:100. 60
Minutes incubation at 25'C. Standard ABC technique.
Positive controls: normal prostate gland or known prostate carcinoma
Staining pattern: cytoplasmic
Storage and stability: Store unopened lyophilized antibody at 4'C. Under
these conditions, there is no significant loss in product performance up
to the expiry date indicated on the vial label. The reconstituted antibody
is stable for at least two months when stored at 4'C. For long term
storage, aliquot in non frost freze freezer, Avoid repeated freeze thaw cycles.
Prepare fresh working dilutions daily. Stricly for in vitro research use
only-Not for use in or on humans oranimals-not for use in diagnostics
cat# RDI-PSA85abm $375.00/100ug
RDI-PSA85abm-1 $500.00/200ug
Presentation: Protein G purified antibody in 10mM PBS with 0.2% BSA and 15mM
NaN3. (Bulk available without BSA/Azide upon request) concentration
200ug/ml
Immunogen: purified human PSA
Isotype: mIgG1 (both)
Clones: ER-PR8 & PA05
Reactivity: human(others not tested)
Uses: -Western blot: 1ug/ml 2 hr rt -Western blot: 1ug/ml 2 hr rt (recommend
clone ER-PR8
alone for best westernblot reactivity cat#RDI-PSAR8abm $438.00/200ug
-histochemistry (frozen and paraffin sections) approx 1-2ug/ml 30-60 minutes RT
-for formalin fixed sections, requires pretreatment in boiling 10mM citrate
bufer pH6.0 for 10-20 minutes followed by cooling at RT for 20 min.
Positive Contrl: prostate or prostate carcinoma
Localization: Cytoplasmic
Background: Recognizes a single protein of 33-34kDa, identified as PSA. This
"cocktail" is composed of two clones whose epitopes are not precisely mapped,
but experimental data suggets that they are different. This cocktail is highly
specific to PSA and stains prostatic secretory and ductal epithelium in both
normal and neoplastic tissues. PSA is a chymotrypsin-like serine protease
(kallikrein family) exclusively produced by the prostate pithelium, and abundant
in seninal fluid. PSA can be detected in the sera of patients with prostatic
carcinoma. It is predominantly complexed to a liver derived serine protease
inhibitor, alpha-1- antichymotrypsin. A higher proportion of serum PSA is
complexed to ACT in prostate cancer than in benign prostate hyperplasia.
Storage: 2-8 deg c
For Research Use Only
San Jose, 95123 CA Snell ave 658
USA
or 408-780-0908
EMAIL:margaret@cellular-research.com
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