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ANTIBODIES  

(anti-Human and others as indicated)

RDI Divison of Fitzgerald Industries Intl  offers a wide line of  antibodies. Since no one antibody works best for all applications (neutralization, blotting, histochemistry, ELISA, etc), we offer many different types of antibodies to help solve this problem. Please inquire for other applications or types of antibodies not listed below. All antibodies are for research use only.


see below for 3 monoclonals to Chromogranin A and polyclonals
NEW! recombinant human Chromogranin A Protein (for use as std in WB or Elisa)

cat#RDI-CHRMGA-AG $469.00/5ug

cat#RDI-CHRMGA-AG $750.00/10ug

-presented in PBS buffer with 0.2% BSA and 0.09% sodium azide

-recombinat protein encoding aa 260-454 (E coli expressed)

-approx 47kda


RDI-CHRMGAbm-COC   $500.00

-cocktail of clone LK2H10 and PHE5

-200ug in 1ml with BSa and 0.1% sodium azide

-ideal for histochemistry (see clone particulars below)


Mouse anti-Chromogranin A clone LK2H10

 cat# RDI-CHRMGA2abm        $375.00/100ug

        RDI-CHRMGA2abm-1     $562.00/200ug

        RDI-CHRMGA2abmX      $625.00/200ug 0.2mg in 0.2ml (NO BSA/NO AZIDE)

        RDI-CHRMGA2abm-BT   $625.00/200ug Biotin labeled

Presentation: Protein G purified antibody in 10mM PBS with 0.2% BSA and 15mM NaN3. (Bulk available without BSA/Azide upon request) concentration 200ug/ml

Clone: LK2H10

Isotype: IgG1

Immunogen: Human pheochromocytoma

Fusion Partner: BALB/c splenocyte and mouse Myeloma p3-NS1-AG4-1 cells

Uses: -histochemistry (frozen and parafin sections) 1:200 for 60 min at room temp. Titer for Optimum use -it is recommended , although not required for paraffin sections to use high temp antigen release with boiling 10mM citrate buffer pH 6.0 for 1-20 min followed by cooling at room temp for 20 min.

-western blot

Positive Control: adrenal gland, normal bowel, or normal thyroid

Localization: finely granular Cytoplasmic

Mapping: recognizes a protein of 68-75kDa, identified as chromogranin A (a protein of 439 amino acids which is encoded on chromosome 14). Although not precisely mapped, experimnetal data shows a different eiptope is seen with clone LK2H10 than clone PHE5.

Species Reactivity: human, monkey and pig. Not with sheep, rabbit, mouse, rat, guinea pig. Others not tested.-some investigators have reported rabbit and rat reactivity (must be tested independently for each application).

ref: Science 1983, 222:628-30.

      Am. J. Pathol. 115, 458 (1984)

Storage: 2-8 deg c


For Research Use Only


Mouse anti-Chromogranin A     clone PHE5

cat# RDI-CHRMGAPabm      $375.00/100ug

        RDI-CHRMGAPabm-1  $562.00/200ug

        RDI-CHRMGAPabmX  $594.00/0.2mg in 0.2ml (No BSA/NO AZIDE) Larger sizes quoted on request

Presentation: Protein G purified antibody in 10mM PBS with   0.2% BSA and 15mM NaN3. (Bulk available without BSA/Azide upon request) concentration 200ug/ml

Clone: PHE5

Isotype: IgG1

Immunogen: Human pheochromocytoma

Fusion Partner: BALB/c splenocyte and mouse Myeloma p3-NS1-AG4-1 cells

Uses: -histochemistry (frozen and parafin sections) 1:200 for 60 min at room temp. Titer for Optimum use -it is recommended , although not required for paraffin sections to use high temp antigen release with boiling 10mM citrate buffer pH 6.0 for 1-20 min followed by cooling at room temp for 20 min.

-western blot 1:500-1:1000

Positive Control: adrenal gland, normal bowel, or normal thyroid

Localization: finely granular Cytoplasmic

Mapping: recognizes a protein of 68-75kDa, identified as chromogranin A (a protein of 439 amino acids which is encoded on chromosome 14). Although not precisely mapped, experimental data shows a different epitope is seen with clone LK2H10 than clone PHE5.

Species Reactivity: human and monkey. Not with rat and mouse. Others not tested.


ref: Ridell K, et al Lab Invest 56:64A, 1987

Am. J. Pathol. 115, 458 (1984)

Storage: 2-8 deg c

For Research Use Only


Additional Chromogranin A references
REFERENCES: Lloyd, R.V. & Wilson, B.S. Science 222, 628 - 30 (1983)

Aguirre, P., Scullx, R.E., Wolfe,H.J., and DeLellis R.A.: Argyrophil cells in Brenner tumors: Histochemical and immuno-histochemical analyses Int. J. Gynecol. Pathol. 5, 223 (1986)

Bussolati, G., Papotti, M., Sapino, A., Gugliotta, P.,Ghiringhello, B, and Azzoparadi, J.G.: Endocrine markers in argyrophilic carcinomas of the breast Am J. Surg. Pathol. 11, 248. (1987)

DeStephano, D. B., Lloyd, R.V., Pike, A.M. and Wilson, B. S.: An immunohistochemical study of hormone production and chromogranin localization Am. J. Pathol. 116, 464.(1984)

Harlow, E. et al. Antibodies: A laboratory manual, p. 359, Cold Spring Laboratory, New York, (1988)

Hearn, S. A.: Electron microscopic localization of chromogranin A in osmium-fixed neuroendocrine cells with a protein A-gold technique J. Histochem. Cytochem. 35, 795 (1987).

Lloyd, R. V., and Wilson, B. S.: Specific endocrine tissue marker defined by a monoclonal antibody science 222, 628 (1983).

McNutt, M. A., M.D. and John, W. Bolen: Adenomatous tumor of the middle ear, An ultrastructural and immunocytochemical Study Am. J. Clin. Pathol. 84, 541 (1985)

Pietroletti, R., Bishop, A.E., Carlei, F., Bonamico, M., Lloyd R.V., Wilson, B.S., Ceccamea, A., Lezoche, E., Speranza, V., and Polark, J. M.: Gut endocrine cell population in coeliac disease estimated by immunocytochemistry using a monoclonal antibody to chromogranin Gut 27, 838 (1986).

Wiedenmann, B., Waldherr, R., Buhr H., Hille, A., Rosa, P., and Huttner, W.B. : Identification of gastroenteropancreatic neuroendocrine cells in Normal and neoplastic human tissue with antibodies against synaptophysin, chromogranin A, Secretogranin I (Chromogranin B), and Secretogranin II Gastroenterology 95, 1364 (1988)

Wilson, B.S., Lloyd, R. V. et al.: Detection of chromogranin in neuroendocrine cells with a monoclonal antibody Am. J. Pathol. 115, 458 (1984) .

Wilson, B.S., Phan, S. H., and Lloyd, R.V.: Chromogranin from normal human adrenal glands: purification by monoclonal antibody affinity chromatopraphy and partial N-terminal amino acid sequence Regulatory Peptides, 13, 207 (1986)


Rabbit (monolconal) Anti-chromogranin A

-cat#RDI-CHRMG-SP12    $375.00/0.5ml

-cat#RDI-CHRMG-SP121  $625.00/1ml  

-clone SP12

-rabbit monoclonal

-supernatant with 0.09% sodium azide

-reactive with human, monkey, pig, rat, and rabbit

-excellent for formalin fixed paraffin sections

-cytoplasmic staining

-positive contro: pancreas


rabbit anti-Chromogranin A RDI-CHRMGAabrX

$500.00/200ug in 1ml PBS with 0.2% BSA and 0.09% sodium azide

-reacts with human, monkey, rat, pig and rabbit (other not tested)

-made with recombinant human chromogranin A

-sutiable for formalin fixed paraffin sections (no special pretreatemnt required)


Rabbit anti- Chromogranin A+B

cat#RDI-PRO11422   $375.00/vial

Category: Rabbit polyclonal

Form/Purification Rabbit serum

Immunogen:    Human pheochromocytoma extract

Clinical Application/ Marker for neuroendocrine, neuronal and Specificity adrenal tumors. In immunohistochemistry, the antiserum gives a specific reaction in pituitary antrum, duodenum, small intestine and pancreas

Recommended for Positive Control: Pancreas, duodenum

Application · Immunohistochemistry  · Suitable for frozen and paraffin-embedded* tissue

                   *no proteolytic treatment required

Antigen Recognized Human, mouse, rat, rabbit

Working Dilution 1:50 - 1:100, optimal dilution should be tested by serial dilution

Dilution Buffer PBS with 0.1% Na-Azide

Incubation Time 1 h at room temperature

Storage At 4°C (undiluted) or at -20°C (aliquots)

Volume 0.25 ml

For Research Use only


RDI Divison of Fitzgerald Industries Intl

34 Junction Square Drive

Concord MA 01742-3049

USA

phone (978) 371-6446 or (800) 370-2222

fax     (978) 371-2266

EMAIL:antibodies@fitzgerald-fii.com

Ordering terms

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