rev:   July 7, 2004

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  ANTIBODIES  

(anti-Human and others as indicated)

RDI Divison of Fitzgerald Industries Intl  offers a wide line of  antibodies. Since no one antibody works best for all applications (neutralization, blotting, ELISA, etc), we offer many different types of antibodies to help solve this problem. Please inquire for other applications or types of antibodies not listed below.

$440.00/1mg      $406.00/mg 2-9mg      $375.00/mg 10+ (unless indicated)

-custom Biotin or HRP labeling available on request

Cardiac Troponin I plays an essential role in diagnosing myocardial damage and is becoming increasingly important as a prognostic marker and in risk stratification of non-ST-elevation ACS patients. This calls for cTnI assays with improved analytical sensitivity and precision. There are many different factors what could influence performance of troponin I assay and affect antibody selection strategy (1). Recently the occurrence of a common interfering factor (IF) that negatively affects determinations of cTnI has been reported (2).

In this Product Release we are describing new monoclonal antibodies to cardiac troponin I which could be suitable for development of interference-free cTnI immunoassay

Reference:

1. Katrukha Alexei "Antibody Selection strategies in cardiac troponin assays" in Cardiac Markers, Humana Press, 2003

2. Susann Eriksson, Miia Junikka, Paivi Laitinen, Kirsi Majamaa-Voltti, Henrik Alfthan, Kim Pettersson "Negative Interference in Cardiac troponin I immunoassays from a frequently occurring serum and plasma component", Clinical Chemistry 49:7, 1095-1104 (2003)

Ordering information:

Product	Cat.#	Clone	Subclass	Remarks
Anti-cardiac troponin I	RDI-TRK4T21-MF4	MF4	IgG1	EIA, WB
Anti-cardiac troponin I	RDI-TRK4T21-M155	M155	IgG1	EIA, WB
Anti-cardiac troponin I	RDI-TRK4T21-M18	M18	IgG1	EIA, WB
Anti-cardiac troponin I	RDI-TRK4T21-19C7	19C7	IgG2b	EIA, WB
Anti-cardiac troponin I	RDI-TRK4T21-8E10	8E10	IgG1	EIA, WB
Anti-cardiac troponin I	RDI-TRK4T21-16A11	16A11	IgG1	EIA, WB
Anti-cardiac Troponin C	RDI-TRK4T27-7B9	7B9	IgG1	EIA, WB

BEST RECOMMENDED PAIRS FOR IMMUNOASSAY (Figure 1)

Coating antibody - Detection antibody :

Note Detection ab would have to be labeled for use in immunoassay, Biotin, HRP or other (not provided labeled unless custom ordered)

M18 (coating) - MF4 (detection)

MF4 (coating) - M155 (detection)

19C7 + M18 (mixture for coating) - MF4 + 8E10 (mixture for detection)

19C7 + M18 (mixture for coating) - MF4+16A11 (mixture for detection)

19C7 + MF4 (mixture for coating) - 7B9 (detection)

19C7     -   23C6,

19C7     -     3C7 (best for immunochromatographic assay)

3C7       -     19C7 (for immunochromatographic assays)

23D10   -     23C6

19C7     -     8E10

19C7     -     8E10

19C7     -     16A11

8E10-19C7, 19C7-8E10, 19C7-16A11, 16A11-19C7, 110-111,   110-7F4, 110-mixture of 111 and 7F4, 2A3-10B11.

-all clones except M46 and C5 have no corss reaction with skeletal muscle troponin I

  *Note: clones C5 100% cross with skeletal tropoin I

             clone M46 has approx 5-7 % cross with skeletal troponin Ialso see skeletal troponin I

NOTE:   clone 22B11 recognizes only dephospho-form of cTNi and does not react with mono or biphosphorylated antigen. cat#RDI-TRK4T46  $469.00/mg  or

clone 5E6 (phosphorylated form) cat#RDI-TRK4T45  $469.00/mg

cat#RDI-TRK4T21-XXXX        XXX=clone number (see below)

$438.00/1mg      $406.00/mg 2-9mg      $375.00/mg 10+

Significant Bulk Discounts Available-please inquire

For detection of troponin I in complex with troponin C:   use clones 7B9 (coating)-19C7 detection   (7B9=anti-troponin C), 19C7-tropinin I

All antibodies work in western blot and in   immunoaffinity purification.

For WB recommend 16A11,   4G6, 19C7, 6F9 or 7F4.

Immunocytochemistry staining protocol

The cells were fixated 100 % by methanol at - 20C during 20 minutes. After washing with PBS cells were incubated at 10 minutes with solution which blocks nonspecific binding of immunoglobulins. Solution contains 3% of horse serum. Further after three-multiple washing with PBS the cells were incubated with mouse monoclonal antibodies against troponin I with dilution 1: 100 within 12 hours at 4C. After three-multiple washing with PBS the cells were incubated with conjugate (rabbit anti-mouse immunoglobulins) labelled with FITC in dilution 1:200 for 45 minutes at room temperature. After washing the cells with PBS, 60 % solution of glycerol has been added and cells were covered with glass.

CLONEs: 8E10, 19C7, 16A11, 110 (10F4), 111, 112, 10B11, 2A3, 3A1, 5F1, 7F4, 11D7, 414: derived from the hybridization of Sp 2/0 myeloma cells with spleen cells of Balb/c mice immunized with native troponin complex

Clone: 8E10, 190F4, 7F4, 6F9, 414, B2 and C5) derived from free human troponin I

Isotypes: B2, C5, 5F1=mIgG2b.

clones 111, 7F4, 10B11, 2A3, 11D7, 414, 3A1, 8E10=mIgG1.

Clones 110, 112,=mIgG2a

19C7-IgG2b.

Cross: B2=troponin I from human, bovine and rabbit heart(). Cross with skeletal muscle troponin I is less than 0.01%. Titer is 10-7.

C5=troponin I from human and bovine heart (also rabbit, mouse, chicken, fish, frog helix heart and skeletal muscle)). Cross reactivity with skeletal muscle troponin I is 5-7%. Titer is 10-6.


110(10B4), 7F4=troponin I from human heart. No cross with skeletal muscle troponin I. Titer >10-7.


111, 10B11, 2A3, 5F1, 11D7=troponin I from human heart. No cross with skeletal muscle                                                          troponin I. Titer >10-6.


112=troponin I from human heart. Some cross with skeletal muscle troponin I is observed. Titer                                                      >10-6


414=troponin I from human heart. Cross with skeletal muscle troponin I is 0.1%. Titer >10-7.

Package: in PBS, pH 7.4 with 0.1% sodium azide as preservative. Store at 4 DEG C.

For In Vitro Research or Further Manufacturing Use Only  Please inquire for other troponin antibodies as well as the pure protein!

Catalogue # RDI-TRK4T45   $469.00/mg

Clone: Clone 5E6

This clone has been derived from hybridization of Sp 2/0 myeloma cells with spleen cells of Balb/c mice immunized with phosphorylated form of cardiac troponin I

Specificity: Phosphorylated form of cardiac troponin I.

Mouse isotype: IgG

Application: Antibodies are working in Western blotting and ELISA.

Purification: Purified by chromatography on protein A Sepharose. Purity is tested by electrophoresis.

Presentation: Each vial contains antibodies in (mg/ml ) PBS, pH 7,4, containing 0,1% of sodium azide as preservative. Concentration and lot numbers are indicated on the vial. Store at +4ºC.

Material safety : This product is sold as an antibody preparation for research purpose only. Standard Laboratory Practices should be followed when handling this material. Contains sodium azide (0,1%) as preservative. Although the amount of sodium azide is very small appropriate care must be taken when handling this product.

For Research Use Only

Catalogue # RDI-TRK4T46   $469.00/mg

Clone: Clone 22B11 (20-24 a.a.r.)

This clone has been derived from hybridization of Sp 2/0 myeloma cells with spleen cells of Balb/c mice immunized with dephosphorylated form of cardiac troponin I

Specificity: Dephosphorylated form of cardiac troponin I, no cross-recation with mono or biphosphorylated troponin I.

Mouse isotype: IgG2b

Application: Detection of dephosphorylated cTnI. Antibodies are working in Western blotting and ELISA.
Detection of dephosphorylated cTnI by Western blotting or by sandwich immunoassay cTnI is phosphorylated in vivo and can be phosphorylated in vitro by cAMP-dependent protein kinase A. Sites of phosphorylation are the serines in the 22 nd and 23 d position. It was demonstrated recently that one of these MAbs - namely MAb 22B11 recognizes only dephospho- form of cTnI and does not interact with mono- or biphosphorylated antigen. 22B11 was suggested to be used for qualitative or semi quantitative immunodetection of dephosphorylated cTnI in Western blotting or for quantitative measurements of dephosphorylated cTnI in sandwich immunoassay. Using such immunoassay we recently demonstrated that significant part of cTnI in patient 's blood is partially phosphorylated.

Purification: Purified by chromatography on protein A Sepharose. Purity is tested by electrophoresis.

Presentation: Each vial contains antibodies in PBS, pH 7,4, containing 0,1% of sodium azide as preservative. Concentration and lot numbers are indicated on the vial. Store at +4ºC.

Material safety note: This product is sold as an antibody preparation for research purpose only. Standard Laboratory Practices should be followed when handling this material. Contains sodium azide (0,1%) as preservative. Although the amount of sodium azide is very small appropriate care must be taken when handling this product.

Catalog#: RDI-TROPIabg

Cost: BULK QUOTES ON REQUEST

         $375.00/ 1- 10mgs

         $312.00/mg 11- 49mg

         $250.00/mg 50-100mg

Batch# RDI/tropi/85

Package Size: 1 milligram in 0.2ml (5 mg/ml) in 10mM PBS   pH 7.2 with 0.15 M NaCl and 0.1% sodium azide

Source: goat

Purity: IgG purified using the N-terminal region peptide (27-40)

Specificity: Specific for cardiac Troponin I, not reactive with human skeletal muscle Troponin I by ELISA.

Application -immunoassay

-All titers must be determined in assay

Storage: Store at 4 DEG C(short term)- 20 DEG C long term. Avoid frequent freeze thaw cycles.

Precautions: For In vitro research Use Only. Not for use in or on humans or animals or for diagnostics.

Source: Prepared in the USA in compliance with USDA regulations at a farm registered under NIH, the USDA animal welfare act and USDA veterinary biologics establishment. Export Certificate for animal products available upon request with shipments (allow extra 7 days for delivery due to time delay in obtaining certificates).

It is the responsibility of the user to comply with all local/state and Federal rules in the use of this product. We are not responsible for any patent infringements that might result with the use of or derivation of this product.

Catalog#: RDI-TROPIC88abg

Cost: BULK QUOTES ON REQUEST

$375.00/ 1- 10mgs      $312.00/mg 11- 49mg    $250.00/mg 50-100mg

Source: goat

Purity: IgG purified using the peptide from aa 80

Specificity: Specific for cardiac Troponin I, not reactive with human skeletal muscle Troponin I by ELISA.

(Coating Antibody)

(affinity purified)

Catalog#: RDI-TROPIabr

Cost: BULK QUOTES ON REQUEST

         $312.00/mg 1- 10mgs

         $219.00/mg 11- 49mg

         $156.00/mg 50-100mg

Batch# see batch sheet

Package Size: 1 milligram

Source: rabbit

Purity: affinity purified by chromatography on human cardiac troponin I immobilized column. Packaged as 1mg in PBS pH 7.4 with 00.1% merthiolate preservative.

Specificity: reacts with troponin I from human heart. Approx 10% cross with skeletal muscle troponin I.

Application -immunoassay:Recommend to use as the coating anibody with monoclonal clone 7F4 (see our troponin monoclonals) to be used as the labelled detecting antibodies. -All titers must be determined in assay

Storage: Store at 4 DEG C.

Precautions: For In vitro research Use Only. Not for use in or on humans or animals or for diagnostics. It is the responsibility of the user to comply with all local/state and Federal rules in the use of this product. We are not responsible for any patent infringements that might result with the use of or derivation of this product.

34 Junction Square Drive

Concord MA 01742-3049

USA

phone (978) 371-6446 or (800) 370-2222

fax     (978) 371-2266

EMAIL:antibodies@fitzgerald-fii.com

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