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rev:February 5, 1997
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The following book on the IgG Subclass is made available by the permisssion
of CLB Reagents (Netherlands). It is for your personal use and may not be
reproduced without the permission of CLB Reagents. This material is for
information use only and should not be construed as medical advice. We are
not responsible for any misprints or error or ommissions. Certain charts
,graphs and table have been ommitted or changed in style (not content)
to fit this web site. Please use this information as part of your discussion
with your medical professional.
2.5 Effector functions of IgG subclasses
The most important biological activities of antibodies are related to their
effector functions, aimed at inactivation or removal of infectious agents
and their products (e.g.bacteria, viruses,toxins). Antibodies of the IgG
class exert two major effector functions:activation of complement and
opsonisation (i.e.the induction of phagocytosis). These effector functions,
mediated via the (constant) Fc fragment are induced as a result of interaction
of the antibody with its antigen via the (variable) Fab moiety.
The four IgG subclasses differ from each other with respect to their effector
functions (table II). This difference is related to differences in structure,
notably with respect to the interaction between the variable,antigen-binding
Fab-fragments and the constant Fc fragment (figure 4). In particular,the
length and flexibility of the hinge region are different. The flexibility
of the hinge region decreases in the order IgG3>IgG1>IgG4>IgG2.
This probably relates to the higher activity of IgG3 in triggering effector
functions,when compared to the other subclasses (21,41).
2.5.1 Complement activation
Serum bactericidal activity is induced when the complete complement cascade
(C1 to C9) is activated. The formation of the membrane attack complex (MAC)
is the final result of activation by the classical pathway or the alternative
pathway of complement.Unlike the classical pathway, activation of the alternative
pathway is not dependent upon antibodies, although many indications exist
for an enhancing activity of antibodies in the alternative pathway. The MAC
(C5b-9) is inserted into the membrane of target cells, after which these
are killed by a lytic mechanism.Besides, opsonisation of micro-organisms
by complement components (C3b, iC3b and C3dg) may result in phagocytosis.
The structural diversity in the hinge region of IgG subclasses entails
differences in their ability to activate complement. The initial event in
the activation of the complement cascade by the classical pathway is the
binding of C1q to sites present on the C(H)2 domains of IgG. This binding
occurs when the latter is aggregated, usually through formation of an
antigen-antibody complex. Other components required for complement activation
include a disulfide bond in the amino-terminal end of C(H)2 (normally the
hinge region), the presence of carbohydrates and paired C(H)3 domains. The
capacity of the four human IgG subclasses (in monomeric form) to bind C1q
decreases in the order: IgG3>IgG1>IgG2>IgG4 (5,31). IgG4 does not
activate complement.Hinge-dependent fab-Fab and Fab-Fc flexibility determines
the accessibility of the complement binding site to C1q.IgG3,the human IgG
subclass with the longest hinge,is the most effective complement activator.
The inability of IgG4 to activate complement is caused by the structure of
the Fc fragment as well as the stearic hindrance of the complement binding
sites by the Fab arms (figure 5).
Although IgG2 is a poor activator of the classical complement cascade, effective opsonisation and killing of certain bacterial strains and protection in bioassays by purified IgG2 have been reported. This may be explained by a combined effect of both bacterial antigen and IgG2 on the activation of complement. Density and accessibility of antigenic determinants on the bacteria may determine the actual complement activation by IgG subclasses. Alternatively,IgG2 may utilize the alternative pathway in complement activation in an Fc-independent way,bypassing C1q binding (table II) (42,43,44). Complement activation via the alternative route appears to be especially important in the opsonisation and killing of bacteria.
2.5.2 Opsonisation and induction of phagocytosis
Most micro-organism are by themselves incapable to activate complement or
to bind phagocytes. Antibodies act as flexible adapters mediating the adherence
of infectious agents to phagocytes. The antibody forms a bridge to attach
the microbe to the Fc receptor on the phagocyte membrane.
Interaction with Fc receptors
Phagocytosis is initiated by an interaction between the Fc fragment of the immunoglobulin and Fc receptors. Fc receptors for IgG (FcgR) are expressed primarily on effector cells of the immune system, in particular macrophages, monocytes,myeloid cells and dendritic cells.
The four human IgG subclasses show differences in their interacttion with FcgR's. Binding of the Fc part of IgG to an FcgR is instrumental in the induction of the cell's effector function(45, 46). In this way, FcgR's play a key role in bridging (IgG) antibody activity and cellular effector mechanisms. The latter comprise e.g. phagocytosis,endocytosis,antibody-mediated cellular cytotoxicity,release of a range of inflammatory mediators,antigen presentation and clearance of immune complexes. Moreover, since several FcgR-bearing cell types (e.g.macrophages,monocytes and dendritic cells), have the capacity to present antigens to T lymphocytes, FcgR-induced phagocytosis also plays a role in antigen presentation and amplification of the immune response. As a result, interaction between FcyR and IgG antibodies is pivotal in the immune response against infectious agents (47). Since IgG subclasses differ in their affinity to FcyR, clinical manifestations of IgG subclass deficiencies will depend upon the properties of the FcgR as well.
Different types of human FcgR
It has been shown that on human leukocytes three distinct types of Fcg-receptors
occur, which are distinguishable by structural and functional properties,
as well as by antigenic structures detected by CD monoclonal antibodies.
They are designated FcgRI, FcgRII,and FcgRIII,respectively and are differentially
expressed on (overlapping) subsets of leukocytes (table II) (48).
FcgRI (CD64), a high-affinity receptor expressed on monocytes, macrophages,neutrophils, myeloid precursors and dendritic cells, comprised isoforms la and lb. FcgRI has a high affinity for monomeric human IgGI and IgG3. Its affinity for IgG4 is about 10 times lower,while it does not bind IgG2. FcgRI does not show genetic polymorphism (48).
FcgRII (CD32), comprised of isoforms lla,llb1,llb2,llb3 and llc,is the most
widely distributed human FcgR type, being expressed on most types of blood
leukocytes, as well as on Langerhans cells, dendritic cells and platelets.
FcgRII is a low-affinity receptor, that only binds aggregated IgG. It is
the only FcgR class able to bind IgG2. FcgRIIa shows genetics polymorphism,
resulting in two distinct allotypes,FcgRlla-H131 and FcgRlla-R131, respectively
(49,50). This functional polymorphism is attributable to a single amino acid
difference: a histidine (H) or an arginine (R) residue at position 131, which
is critical for IgG binding. FcgRlla readily binds human IgG and IgG3 and
appears not to bind IgG4. The FcgRlla-H131 has a much higher affinity for
complexed IgG2 than the FcyRlla-R131 allotype (50).
FcgRlll (CD16) has two isoforms,both of which are able to bind IgG1 and IgG3. The FcyRlla, with an intermediate affinity for IgG, is expressed on macrophages, monocytes,natural killer (NK) cells and subsets of T cells. FcyRlllb is a low-affinity receptor for IgG, selectively expressed on neutrophils. It is a highly mobile receptor with efficient collaboration with other membrane receptors. Studies with myeloma IgG dimers have shown that only IgG1 and IgG3 bind to FcyRlllb (with low affinity), while no binding of IgG2 and IgG4 has been found (51,52). The FcgRlllb bears a co-dominant, bi-allelic polymorphism, the allotypes being designated NA1 (Neutrophil Antigen) and NA2(53).
FcgR genetic polymorphism, IgG subclasses and disease In human, IgG2 antibodies
have been found to play a key role in immunity against infection with
encapsulated bacteria. This defence does not only depend upon complement
activation,since IgG2 has a poor complement-activating activity, at least
when the epitope density is low. Indeed, it has been shown that phagocytosis
by neutrophil granulocytes is the major underlying mechanism. Moreover, the
FcgRlla polymorphism is important,neutrophils from FcgRlla-H131 homozygous
individuals phagocytising better than those of FcgRlla-R131 individuals(54).
This polymorphism will be more critical when IgG2 levels are low, as is the
case in children. In children surviving meningococcal septic shock, the allotype
FcgRlla-R131 occurred twice as often as in normal donors (47).
Figure 6 (bottom of page) schematically illustrates the role of IgG2 in the defence against micro-organisms and the different underlying routes The first route involves the activation of complement, either by the classical pathway (C1,C4 and C2), or by the alternative pathway (which is potentiated by antibodies of the IgG2 subclass, whereas it should be kept in mind that IgG2 is a poor activator of the classical pathway). In the alternative pathway,bacterial components (indicated here as lipopolysaccharide, LPS) also play an essential role. Activation of the complement cascade, either by the classical or the alternative pathway, will lead to the activation of complement component C3. The binding of activated C3 to the bacterial membrane will lead either to direct killing (mediated by the complement cascade C5 to C9), or to opsonisation resulting in binding to complement receptors on polymorphonuclear cells (PMN),followed by phagocytosis.
According to the second route indicated in figure 6, bacteria sensitized by IgG2 antibodies may also be phagocytised when they bind to the FcyRlla on the cell membrane of the PMN. This latter process will only be effective in individuals expressing a FcgRlla of the allotype H131,since the other allele, FcgRlla-R131, is unable to bind IgG2 effectively.
With respect to FcgRlllb polymorphism, it was reported that the combined
FcgRlla-R131 and FcgRlllb-NA2 phenotype is associated with meningococcal
infections (55).
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