rev: January 23, 2003

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CD CLUSTERED ANTIBODIES  

(anti-Human and others as indicated)

Please inquire for bulk or custom formulations (without preservative or carrier).

RDI Division of Fitzgerald Industries Intl  offers a wide line of  antibodies. Since no one antibody works best for all applications (flow cytometry, neutralization, blotting, histochemistry, ELISA, etc), we offer many different types of antibodies to help solve this problem. Please inquire for other applications or types of antibodies not listed below. All products are for in vitro research use only-not for use in or on humans or animals-not for use in diagnostics. Price/availability/specifications subject to change without notice.


CD8 Antibodies


Mouse anti-human CD8 antibodies

-5 clones     CLB-T8/4,4H8 (purified, FITC & PE)

                    UCHT4 (purified ,FITC & PE) 

                    ASH      (purified & FITC )

                    RFT-8G (purified, FITC & PE)

                    RPA-T8 (purified) & HIT8A

                    LT-8

-for paraffin sections see clone 4B11 ,


CATALOG#                 CLONE#                Workshop  Host       Form        Price

RDI-M1312clb              CLB-T8/4,4H8            IV         mIgG1     purified     $375.00

RDI-M1468clb                " "                               "             "           FITC         $375.00

RDI-M1629clb                " "                               "             "           PE             $500.00

RDI-CBL131                 UCHT4                        I           mIgG2a   purified     $375.00  

RDI-CBL492FT              " "                               --             "          FITC        $375.00

RDI-CBL492PE              " "                                "              "          PE           $469.00

RDI-CD8-RPAT8           RPA-T8                      IV        mIgG1     purified    $281.00

RDI-CD8abm-LT8          LT8                             V,VII   mIgG1     purified    $438.00/1mg

    **(Cytolytic complement fixing clone)


Product Specification: mouse and human CD8

Code: CLB-CD8 (CLB-T8)

CAT#: RDI-M1312clb
Lot number:
Tests/vial: 200
Specificity:Cell type :Human T lymphocytes Ag.MW.: 300 kD.

Reactivity:The monoclonal antibody is directed against the CD8-antigen (T8-antigen),which is expressed on human T lymphocytes. The monoclonal antibody reacts with 20-30% of human peripheral T lymphocytes. The monoclonal antibody reacts with T lymphocytes with suppressor-cell activity in pokeweed mitogen- stimulated immunoglobulin production, as was shown in separation experiments (i.e. "panning"). The monoclonal antiody does not react with B-cells, monocytes, granulocytes and platelets.

Applications:

Monitoring of T-cell numbers in peripheral blood.

Characterization of leukaemias and lymphomas. Identi fication of T-cells in tissue.

Methods: Indirect immunofluorescence staining with analysis by FACS or fluorescence microscopy.

Clone: CLB-215

This clone has been derived from hybridization of SP2/0 cells with spleen cells of a (BALB/c x A/J) mouse immunized with human T lymphocytes.

Mouse isotype: The monoclonal antibody is of the mouse subclass IgG2b.

Source: Ascites fluid of tumour bearing BALB/c mice.

Purification: Ammoniumsulphate precipitation and affinity chromatography.

Packing: Each vial contains 1 ml with approximately 0.025 mg/ml monoclonal antibody and 10 mg BSA in 20 mM NaCl, pH 8.0.

Preservative: Merthiolate (0.001%).

Storage: Monoclonal antibodies should be stored below -18C. Repeated thawing and freezing may reduce the antibody activity and should be avoided.

FOR IN VITRO RESEARCH USE ONLY


mouse monoclonal anti-human CD8,conjugated to Fluorescein

Code: CLB-CD8-F (CLB-T8)   CAT#: RDI-M1468clb
Lot Number:
Tests/vial: 100
Specificity:Cell Type : Human T lymphocytes.Ag.MW. : 30 kD.


Reactivity: The monoclonal antibody is directed against the CD8- antigen),which is expressed on human T lymphocytes. The monoclonal antibody reacts with 20-30% of human peripheral T lymphocytes. The monoclonal antibody reacts with T lymphocytes with surpressor-cell activity in pokeweel mitogen- stimulated immunoglobulin production, as was shown in separation experiments (i.e."panning"). The monoclonal antibody does not react with B-cells, monocytes, granulocytes and platelets.

Methods: Direct immunofluorescence staining with analysis by FACS or fluoresence microscopy.

Clone: CLB-215

This clone has been derived from hybridization of SP2/0 cells with spleen cells of a (BALB/c x A/J) mouse immunized with human T lymphocytes.

Mouse isotype: The monoclonal antibody is of the mouse subclass IgG2b.

Source: Ascites fluid of tumor bearing BALB/c mice.

Purification:Ammoniumsulphate precipitation and affinity chromatography.


Chemical Characteristics:

Molecular F/P ratio : 8.8

Weight F/P ratio : 2.3 x 10-2

Fluorescein concentration : 2.3 x 10-4 mg/ml.

Packing: Each vial contains 1 ml with approximately 10ug/ml conjugated monoclonal antibody and 10 mg BSA in PBS.

Preservative:Metthiolate (0.001%).

Storage: Monoclonal antibodies should be stored at -20C. Re peated thawing and freezing may reduce the antibody activity and should be avoided.


ANTI-HUMAN CD8+ Suppressor/Cytotoxic T Lymphocytes


PRODUCT CODE :RDI-CBL 131  $375.00/vial

CLONE: UCH-T4

ISOTYPE: IgG2a

SOURCE: Mouse ascitic fluid


PURIFICATION METHOD: Ion exchange chromatography following ammonium sulphate precipitation.


SPECIFICITY: 30/32 kD MW lymphocyte surface antigen identified by monoclonal antibodies belonging to the CD8 cluster on a sub-population of peripheral blood T lymphocytes, 60% of thymocytes, and a limited number of malignancies of T cell origin. Normal B lymphocytes, monocytes or granulocytes do not express surface CD8 antigen.

The CD8 positive T lymphocyte subpopulation has been characterised functionally as containing suppressor cells able to down regulate immune responses and also cells possessing cytotoxic activity.

Antigen distribution: Thymocytes 60 ± 10%

                               T-cells (E+) 32 ± 8%

                               Peripheral blood lymphocytes 22 ± 9%

                               NK-cells (CD16+) 18 ± 8%

                               B-cells (E-) < 1%

                               Monocytes < 1%

                               Granulocytes < 1%


APPLICATIONS:

* Identification of CD8 positive suppressor/cytotoxic T lymphocytes by indirect immunofluorescence staining

* Leukaemia and lymphoma phenotyping

* Identification of normal, reactive and malignant CD8 positive T lymphocytes in cryostat sections.

* Isolation or removal of suppressor/cytotoxic T lymphocytes by FACS or panning techniques

* Immunoprecipitation of the CD8 antigen



REFERENCES: LEUCOCYTE TYPING I, Eds. Bernard, A. et al .(Springer- Verlag) 1984, pp 25-60.

PRESENTATION: Each monoclonal is delivered in a sealed vial at a concentration of not less than 200µg/2ml in phosphate buffered saline containing 10mM sodium azide and 1mg/ml of bovine serum albumin. We recommend that each laboratory determine an optimum working titre for use in its particular application.

STORAGE: Purified monoclonal antibodies are generally stable for long periods at 4oC. However repeated warming to room temperature and re-cooling may result in a loss of activity and hence effective working titre. It is recommended that monoclonal antibodies are aliquotted upon receipt and then stored at -20C. After thawing they should then be used as a stock solution for up to 60 days. Dilute working solutions are best prepared on the day of use.

For research use only. Not supplied for use in human diagnostic or therapeutic procedures


ANTI-Human CD8+ Suppressor/Cytotoxic T Lymphocytes


PRODUCT CODE :RDI-CBL505 $375.00/vial

also available FITC labeled: cat#RDI-CBL505FT $375.00/vial


CLONE: ASH

ISOTYPE: IgM

SOURCE: tissue culture supernatant

PURIFICATION METHOD: ammonium sulphate precipitation followed by gel filtration


SPECIFICITY: 30/32 kD MW lymphocyte surface antigen identified by monoclonal antibodies belonging to the CD8 cluster on a sub-population of peripheral blood T lymphocytes, 60% of thymocytes, and a limited number of malignancies of T cell origin. Normal B lymphocytes, monocytes or granulocytes do not express surface CD8 antigen.

The CD8 positive T lymphocyte subpopulation has been characterised functionally as containing suppressor cells able to down regulate immune responses and also cells possessing cytotoxic activity.

Antigen distribution: Thymocytes 60 ± 10%

T-cells (E+) 32 ± 8%

Peripheral blood lymphocytes 22 ± 9%

NK-cells (CD16+) 18 ± 8%

B-cells (E-) < 1%

Monocytes < 1%

Granulocytes < 1%


APPLICATIONS: * Identification of CD8 positive suppressor/cytotoxic T lymphocytes by indirect immunofluorescence staining

                              * Leukaemia and lymphoma phenotyping

                              * Identification of normal, reactive and malignant CD8 positive T lymphocytes in cryostat sections.

                              * Isolation or removal of suppressor/cytotoxic T lymphocytes by FACS or panning techniques

                               *Cytolytic complement fixing clone


PRESENTATION: Each monoclonal is delivered in a sealed vial at a concentration of not less than 200µg/2ml in phosphate buffered saline containing 10mM sodium azide and 1mg/ml of bovine serum albumin. We recommend that each laboratory determine an optimum working titre for use in its particular application.


STORAGE: Purified monoclonal antibodies are generally stable for long periods at 4oC. However repeated warming to room  temperature and re-cooling may result in a loss of activity and hence effective working titre. It is recommended that monoclonal antibodies are aliquotted upon receipt and then stored at 4 DEGC (up to one month). For longer storage, add equal volume of glycerol and store at -20 DEG C. Dilute working solutions are best prepared on the day of use.

Also available in BULK, without BSA and without azide CUSTOM QUOTES WELCOME

order cat#RDI-CBL506-1XP $1000.00/1mg   $750.00/mg 2-10 $562.00/mg 11-50mg Larger sizes available!
Also available cytolytic abs for CD6, CD19, CD10, CD37 & CD105 same pricing


For research use only. Not supplied for use in human diagnostic or therapeutic procedures



Rat anti-mouse CD8 A clone 53-6.72

cat#RDI-MCD8A-53 $250.00/100ug

cat#RDI-MCD8A-53FT $281.00/100ug Biotin labeled

cat#RDI-MCD8A-53FT $281.00/100T FITC labeled

cat#RDI-MCD8A-53PE $406.00/120T

-rat IgG2a subclass


Monoclonal mouse anti-CD8 clone LT8

Catalog # RDI-CD8abm-LT8     $438.00/mg Bulk on request

Clone :                        clone LT8        This clone has been derived from hybridization of X63.653 myeloma with spleen cells of BALB/c mice immunised with normal human blood lymphocytes.

Catalogue No:             RDI-CD8abm-LT8 $438.00/1mg6L8n

Antigen distribution:  CD8 is present on 23+4% of normal peripheral blood lymphocytes and 80% of thymocytes. This antigen is found on T suppressor/cytotoxic T cells.

Specificity :                Clone LT8 reacts with CD8 antigen (MW 32 kDa). LT8 completely cross blocks the binding of MAbs Leu 2a and OKT8. Working dilution 1:5000.

Mouse isotype :           IgG1

Application :               Can be used to enumerate T supressor/cytotoxic subset in peripheral blood and tissue.

Presentation:              Each vial contains antibodies, in PBS, pH 7,4, containing 0,1% sodium azide as preservative. Store at + 4 °C.

Material safety note: This product is sold as an antibody preparation for research purpose only. Standard Laboratory Practices should be followed when handling this material. Contains sodium azide (0,1%) as preservative. Although the amount of sodium azide is very small appropriate care must be taken when handling this product.

For research Use Only


RDI Division of Fitzgerald Industries Intl

34 Junction Square Drive

Concord MA 01742-3049

USA

phone (800) 370-2222

     or  (978) 371-6446 or (800) 370-2222

fax     (978) 371-2266

EMAIL:antibodies@fitzgerald-fii.com

Ordering terms

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