rev: J5/16/2008
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CD CLUSTERED ANTIBODIES
(anti-Human and others as indicated)
Please inquire for bulk or custom formulations (without preservative or carrier).
RDI Division of Fitzgerald Industries Intl offers a wide line of antibodies. Since no one antibody works best for all applications (flow cytometry, neutralization, blotting, histochemistry, ELISA, etc), we offer many different types of antibodies to help solve this problem. Please inquire for other applications or types of antibodies not listed below. All products are for in vitro research use only-not for use in or on humans or animals-not for use in diagnostics. Price/availability/specifications subject to change without notice.
CD8
Mouse anti-human CD8 antibodies
-5 clones CLB-T8/4,4H8 (purified, FITC & PE)
UCHT4 (purified ,FITC & PE)
ASH (purified & FITC )
RFT-8G (purified, FITC & PE)
RPA-T8 (purified) & HIT8A
LT-8
-for paraffin sections see clone 4B11 ,
CATALOG# CLONE# Workshop Host Form Price
RDI-M1312clb CLB-T8/4,4H8 IV mIgG1 purified $375.00
RDI-M1468clb " " " " FITC $375.00
RDI-M1629clb " " " " PE $500.00
RDI-CBL131 UCHT4 I mIgG2a purified $412.50
RDI-CBL492FT " " -- " FITC $375.00
RDI-CBL492PE " " " " PE $469.00
RDI-CD8-RPAT8 RPA-T8 IV mIgG1 purified $281.00
RDI-CD8abm-LT8 LT8 V,VII mIgG1 purified $438.00/1mg
**(Cytolytic complement fixing clone)
Product Specification: mouse and human CD8
Code: CLB-CD8 (CLB-T8)
CAT#: RDI-M1312clb
Tests/vial: 200
Specificity:Cell type :Human T lymphocytes Ag.MW.: 300 kD.
Reactivity:The monoclonal
antibody is directed against the CD8-antigen (T8-antigen),which is expressed on
human T lymphocytes. The monoclonal antibody reacts with 20-30% of human
peripheral T lymphocytes. The monoclonal antibody reacts with T lymphocytes
with suppressor-cell activity in pokeweed mitogen-
stimulated immunoglobulin production, as was shown in separation experiments
(i.e. "panning"). The monoclonal antiody
does not react with B-cells, monocytes, granulocytes
and platelets.
Applications:
Monitoring of T-cell numbers in peripheral blood.
Characterization of leukaemias
and lymphomas. Identi fication of T-cells in tissue.
Methods: Indirect immunofluorescence staining with
analysis by FACS or fluorescence microscopy.
Clone: CLB-215
This clone has been derived from hybridization of SP2/0 cells with spleen
cells of a (BALB/c x A/J) mouse immunized with human T lymphocytes.
Mouse isotype: The monoclonal antibody is of the
mouse subclass IgG2b.
Source: Ascites fluid of tumour
bearing BALB/c mice.
Purification: Ammoniumsulphate precipitation and
affinity chromatography.
Packing: Each vial contains 1 ml with approximately 0.025 mg/ml monoclonal
antibody and 10 mg BSA in 20 mM NaCl,
pH 8.0.
Preservative: Merthiolate (0.001%).
Storage: Monoclonal antibodies should be stored below -18C. Repeated thawing
and freezing may reduce the antibody activity and should be avoided.
FOR IN VITRO RESEARCH USE ONLY
mouse monoclonal anti-human
CD8,conjugated to Fluorescein
Code: CLB-CD8-F (CLB-T8) CAT#: RDI-M1468clb
Lot Number:
Tests/vial: 100
Specificity:Cell Type : Human T lymphocytes.Ag.MW.
: 30 kD.
Reactivity: The monoclonal antibody is directed against the CD8- antigen),which is expressed on human T lymphocytes. The monoclonal
antibody reacts with 20-30% of human peripheral T lymphocytes. The monoclonal
antibody reacts with T lymphocytes with surpressor-cell
activity in pokeweel mitogen-
stimulated immunoglobulin production, as was shown in separation experiments (i.e."panning"). The monoclonal antibody does not
react with B-cells, monocytes, granulocytes and
platelets.
Methods: Direct immunofluorescence staining with
analysis by FACS or fluoresence microscopy.
Clone: CLB-215
This clone has been derived from hybridization of SP2/0 cells with spleen
cells of a (BALB/c x A/J) mouse immunized with human T lymphocytes.
Mouse isotype: The monoclonal antibody is of the
mouse subclass IgG2b.
Source: Ascites fluid of tumor bearing BALB/c mice.
Purification:Ammoniumsulphate
precipitation and affinity chromatography.
Chemical Characteristics:
Molecular F/P ratio : 8.8
Weight F/P ratio : 2.3 x 10-2
Fluorescein concentration :
2.3 x 10-4 mg/ml.
Packing: Each vial contains 1 ml with approximately 10ug/ml conjugated
monoclonal antibody and 10 mg BSA in PBS.
Preservative:Metthiolate
(0.001%).
Storage: Monoclonal antibodies should be stored at -20C. Re peated
thawing and freezing may reduce the antibody activity and should be avoided.
ANTI-HUMAN CD8+ Suppressor/Cytotoxic T Lymphocytes
PRODUCT CODE :RDI-CBL 131 $375.00/vial
CLONE: UCH-T4
ISOTYPE: IgG2a
SOURCE: Mouse ascitic fluid
PURIFICATION METHOD: Ion exchange chromatography following ammonium sulphate precipitation.
SPECIFICITY: 30/32 kD MW lymphocyte surface antigen
identified by monoclonal antibodies belonging to the CD8 cluster on a
sub-population of peripheral blood T lymphocytes, 60% of thymocytes,
and a limited number of malignancies of T cell origin. Normal B lymphocytes, monocytes or granulocytes do not express surface CD8
antigen.
The CD8 positive T lymphocyte subpopulation has been characterised functionally as containing suppressor cells able to down regulate immune responses and also cells possessing cytotoxic activity.
Antigen distribution: Thymocytes 60 ± 10%
T-cells (E+) 32 ± 8%
Peripheral blood lymphocytes 22 ± 9%
NK-cells (CD16+) 18 ± 8%
B-cells (E-) < 1%
Monocytes < 1%
Granulocytes < 1%
APPLICATIONS:
* Identification of CD8 positive suppressor/cytotoxic T lymphocytes by indirect immunofluorescence staining
* Leukaemia and lymphoma phenotyping
* Identification of normal, reactive and malignant CD8 positive T lymphocytes in cryostat sections.
* Isolation or removal of suppressor/cytotoxic T lymphocytes by FACS or panning techniques
* Immunoprecipitation of the CD8 antigen
REFERENCES: LEUCOCYTE TYPING I, Eds. Bernard, A. et al .(Springer-
Verlag) 1984, pp 25-60.
PRESENTATION: Each monoclonal is delivered in a sealed vial at a concentration
of not less than 200µg/2ml in phosphate buffered saline containing 10mM
sodium azide and 1mg/ml of bovine serum albumin. We
recommend that each laboratory determine an optimum working titre
for use in its particular application.
STORAGE: Purified monoclonal antibodies are generally stable for long periods
at 4oC. However repeated warming to room temperature and re-cooling may result
in a loss of activity and hence effective working titre.
It is recommended that monoclonal antibodies are aliquotted
upon receipt and then stored at -20C. After thawing they should then be used as
a stock solution for up to 60 days. Dilute working solutions are best prepared
on the day of use.
For research use only. Not supplied for use in human
diagnostic or therapeutic procedures
ANTI-Human CD8+ Suppressor/Cytotoxic T Lymphocytes
PRODUCT CODE :RDI-CBL505 $375.00/vial
also available FITC labeled: cat#RDI-CBL505FT $375.00/vial
CLONE: ASH
ISOTYPE: IgM
SOURCE: tissue culture supernatant
PURIFICATION METHOD: ammonium sulphate precipitation followed by gel filtration
SPECIFICITY: 30/32 kD MW lymphocyte surface antigen
identified by monoclonal antibodies belonging to the CD8 cluster on a
sub-population of peripheral blood T lymphocytes, 60% of thymocytes,
and a limited number of malignancies of T cell origin. Normal B lymphocytes, monocytes or granulocytes do not express surface CD8
antigen.
The CD8 positive T lymphocyte subpopulation has been characterised functionally as containing suppressor cells able to down regulate immune responses and also cells possessing cytotoxic activity.
Antigen distribution: Thymocytes 60 ± 10%
T-cells (E+) 32 ± 8%
Peripheral blood lymphocytes 22 ± 9%
NK-cells (CD16+) 18 ± 8%
B-cells (E-) < 1%
Monocytes < 1%
Granulocytes < 1%
APPLICATIONS: * Identification of CD8 positive suppressor/cytotoxic
T lymphocytes by indirect immunofluorescence staining
* Leukaemia and lymphoma phenotyping
* Identification of normal, reactive and malignant CD8 positive T lymphocytes in cryostat sections.
* Isolation or removal of suppressor/cytotoxic T lymphocytes by FACS or panning techniques
*Cytolytic complement fixing clone
PRESENTATION: Each monoclonal is delivered in a sealed vial at a concentration
of not less than 200µg/2ml in phosphate buffered saline containing 10mM
sodium azide and 1mg/ml of bovine serum albumin. We
recommend that each laboratory determine an optimum working titre
for use in its particular application.
STORAGE: Purified monoclonal antibodies are generally stable for long periods
at 4oC. However repeated warming to room temperature
and re-cooling may result in a loss of activity and hence effective working titre. It is recommended that monoclonal antibodies are aliquotted upon receipt and then stored at 4 DEGC (up to
one month). For longer storage, add equal volume of glycerol and store at -20
DEG C. Dilute working solutions are best prepared on the day of use.
Also available in BULK, without BSA and without azide
CUSTOM QUOTES WELCOME
order cat#RDI-CBL506-1XP $1000.00/1mg
$750.00/mg 2-10 $562.00/mg 11-50mg Larger sizes available!
Also available cytolytic abs for CD6, CD19, CD10,
CD37 & CD105 same pricing
For research use only. Not supplied for use in human
diagnostic or therapeutic procedures
Rat anti-mouse CD8 A clone 53-6.72
cat#RDI-MCD8A-53 $250.00/100ug
cat#RDI-MCD8A-53FT $281.00/100ug Biotin labeled
cat#RDI-MCD8A-53FT $281.00/100T FITC labeled
cat#RDI-MCD8A-53PE $406.00/120T
-rat IgG2a subclass
Monoclonal mouse anti-CD8 clone LT8
Catalog # RDI-CD8abm-LT8 $438.00/mg Bulk on request
Clone : clone LT8 This clone has been derived from hybridization of X63.653 myeloma with spleen cells of BALB/c mice immunised with normal human blood lymphocytes.
Catalogue No: RDI-CD8abm-LT8 $438.00/1mg6L8n
Antigen distribution: CD8 is present on 23+4% of normal peripheral blood lymphocytes and 80% of thymocytes. This antigen is found on T suppressor/cytotoxic T cells.
Specificity : Clone LT8 reacts with CD8 antigen (MW 32 kDa). LT8 completely cross blocks the binding of MAbs Leu 2a and OKT8. Working dilution 1:5000.
Mouse isotype : IgG1
Application : Can be used to enumerate T supressor/cytotoxic subset in peripheral blood and tissue.
Presentation: Each vial contains antibodies, in PBS, pH 7,4, containing 0,1% sodium azide as preservative. Store at + 4 °C.
Material safety note: This product is sold as an antibody preparation for research purpose only. Standard Laboratory Practices should be followed when handling this material. Contains sodium azide (0,1%) as preservative. Although the amount of sodium azide is very small appropriate care must be taken when handling this product.
For research Use Only
RDI Division of Fitzgerald Industries Intl
phone (800) 370-2222
or (978) 371-6446 or (800) 370-2222
fax (978) 371-2266
EMAIL:antibodies@fitzgerald-fii.com
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