rev:   September 9, 2004

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CD CLUSTERED ANTIBODIES  

(anti-Human and others as indicated)

NOTE: Please inquire for bulk or custom formulations (without preservative or carrier).

RDI Division of Fitzgerald Industries Intl  offers a wide line of  antibodies. Since no one antibody works best for all applications (flow cytometry, neutralization, blotting, histochemistry, ELISA, etc), we offer many different types of antibodies to help solve this problem. Please inquire for other applications or types of antibodies not listed below. All products are for in vitro research use only-not for use in or on humans or animals-not for use in diagnostics. Price/availability/specifications subject to change without notice.


CD42b Antibodies


Mouse anti-human CD42b antibodies

-4 clones 


CATALOG#                 CLONE#                  Workshop    Host         Form        Price

RDI-M1539clb               CLB-MB45                    IV          mIgG1      purified      $375.00

RDI-M1638clb                  " "                                 "               "            FITC        $375.00

RDI-M1729clb                  " "                                 "               "             PE            $438.00

RDI-CBL166                   AK2                              V           mIgG1      purified     $375.00

RDI-CBL166PE                 " "                                "              "             PE            $500.00

RDI-CBL480                   MM2/174                     IV          mIgG1       purified     $375.00

RDI-CBL480PE                 " "                                "              "             PE            $469.00

RDI-CD42BHIP1              HIP1                            IV          mIgG1       purified    $281.00


Product Specification: mouse monoclonal anti-Human CD42b

PeliCluster CD42b

cat#RDI-M1539clb


Test/vial 200

Clone CLB-MB45

This clone has been derived from hybridization of SP2/0 cells with spleen cells of a BALB/c mouse. This antibody has been clustered to CD42b in the fourth international Workshop on Human White Cell differentiation Antigens in Vienna (1989).


Isotype Mouse IgG1.


Source Ascites fluid of tumour bearing BALB/c mice.


Purification Ammoniumsulphate precipitatation and ion exchange chromatography.


Packing Each vial contains 1 ml with approximately 0.2 mg/ml monoclonal antibody and 10 mg BSA in 20 mM TRIS and 150 mM NaCl, pH 8.0.


Preservative : 0.1% sodium azide


Storage and stability Monoclonal antibodies should be stored in the dark at 2-8°C. The reagent is stable until the expiry date stated on the vial label.

Major reactivity The monoclonal antibody is directed against the CD42b- antigen (gplb), which is expressed on human thrombocytes and megakaryocytes.


Molecular mass 25, 135 kDa.


Application The monoclonal antibody is a useful marker for megakaryoblasts and megakaryoblastic leukaemias.


Methods Indirect immunofluorescence staining with analysis by flowcytometry or fluorescence microscopy.



Application FOR AN INDIRECT IMMUNOFLUORESCENCE TECHNIQUE



Reagents

- FITC-conjugated goat anti-mouse immunoglobulin antiserum (M1204).

   Remove aggregates by centrifugation at 1000 x g for 10 minutes.

- Buffer: Phosphate Buffered Saline (PBS), containing 0.2% Bovine Serum Albumin (BSA).

- PFA: Para-Formaldehyde 1%, pH 7.2, containing 0.2% BSA.

- Microtiter plates (96 wells, V bottom) or tubes.

Procedure

1   Fixate the cells with PFA 1% and wash them with buffer.

2   Prepare a mononuclear cell suspension with a concentration of 1 x 10 7 cells/ml in buffer.

3   Add 45 µl of cell suspension to the microtiter wells or tubes.

4   Add 5 µl monoclonal antibody to the microtiter wells or tubes and mix gently.

5   Incubate for 30 minutes at 2-8 C.

6   Add 100 µl buffer to the microtiter wells or 2 ml buffer to the tubes and centrifuge at 500 x g for 5 minutes.

7   Aspirate the supernatant from the cell pellet and resuspend the cells.

8   Add 200 µl buffer to the microtiter wells or 2 ml buffer to the tubes and centrifuge at 500 x g for 5 minutes.

9   Aspirate the supernatant from the cell pellet and resuspend the cells.

10 Add 50 µl FITC-conjugated goat anti-mouse immunoglobulin serum, solid phase adsorbed with human immunoglobulins,       diluted 1:80.

11  Incubate for 30 minutes at 2-8 C.

12  Repeat step 6-9.

13 Add 200 µl buffer.

14 The number of positive cells can be determined by flowcytometry analysis or by means of fluorescence microscopy.

NOTE: Care should be taken when drawing blood to avoid activation of platelets.
Platelets activation markers are weakly expressed on platelets prepared from directly fixed normal whole blood.

The expression is maximal on thrombin (1 U/ml) stimulated washed platelets.


FOR IN VITRO RESEARCH USE ONY


Product Specification: mouse monoclonal CD42b,conjugated to

Fluorescein

PeliCluster  CD42b F

cat#RDI-M1638clb

Test/vial 100

Clone CLB-MB45

This clone has been derived from hybridization of SP2/0 cells with spleen cells of a BALB/c mouse immunized with human platelets. This antibody has been clustered to CD42b in the fourth international Workshop on Human White Cell differentiation Antigens in Vienna (1989).


Isotype Mouse IgG1.


Source Ascites fluid of tumour bearing BALB/c mice.


Purification Ammoniumsulphate precipitation and ion exchange chromatography.


Conjugation Conjugated with fluorescein iso thiocyanate isomer 1 (FITC).

Molecular F/P ratio between 5.5 - 9.5.


Packing Each vial contains 1 ml FITC conjugated monoclonal antibody and 10 mg BSA in PBS.


Preservative Mertiolate (0.001%).

Storage and stability Monoclonal antibodies should be stored in the dark at 2-8°C. The reagent is stable until the expiry date stated on the vial label.

Major reactivity The monoclonal antibody is directed against the CD42b- antigen (gplb), which is expressed on human thrombocytes and megakaryocytes.


Molecular mass 25, 135 kDa.


Application The monoclonal antibody is a useful marker for megakaryoblasts and megakaryoblastic leukaemias.


Methods Direct immunofluorescence staining with analysis by flowcytometry or fluorescence microscopy.

FOR IN VITRO RESEARCH USE ONLY


Mouse ANTI- HUMAN CD 42b ANTIGEN

PRODUCT CODE : RDI-CBL 166     $375.00/vial

Also available: FITC labeled cat#RDI-CBL166FT    $375.00/100 Test - DISCONTINUED

                           PE labeled #RDI-CBL166PE        $500.00/100 Test
CLONE: AK2

ISOTYPE: IgG1

Immunizing Antigen: human platelet glycoproteins

Fusion Partner: NS1 myeloma cell line

SOURCE: Mouse ascitic fluid

PURIFICATION METHOD: Protein A affinity chromatography

SPECIFICITY: The antibody is directed against the CD42b antigen, platelet glycoprotein gp1bA, that serves as a receptor for Von Willebrand factor (vWF) and as a high affinity thrombin receptor. AK2 immunblots a band of 130kDa on SDS gels of platelet lysates and gives surface immunofluorescence with fixed or unfixed platelets. The antigen is absent in very low levels on platelets with the Bernard-Soulier syndrome..


APPLICATIONS: * Immunofluorescence studies of platelets, reacts with gp1ba molecule.

                              * Studies with platelets and magakaryocytes in frozen sections by immunohistochemistry.

                              * Functional studies involving von Willebrands adhesion receptor.

                                 -this antibody blocks vWf binding to washed platelets.

REFERENCES: Berndt, M.C. et al. Biochemistry 27, 633-40 (1987)

                           Leucocyte Typing V, Oxford University Press (1995)


PRESENTATION: Each monoclonal is delivered in a sealed vial at a concentration of not less than 200µg/2ml in phosphate buffered saline containing 10mM sodium azide and 1mg/ml of bovine serum albumin. We recommend that each laboratory determine an optimum working titre for use in its particular application.

STORAGE: Purified monoclonal antibodies are generally stable for long periods at 4oC. However repeated warming to room temperature and re-cooling may result in a loss of activity and hence effective working titre. It is recommended that monoclonal antibodies are aliquotted upon receipt and then stored at -200C. After thawing they should then be used as a stock solution for up to 60 days. Dilute working solutions are best prepared on the day of use.


For research use only. Not supplied for use in human diagnostic or therapeutic procedures


MOUSE ANTI -HUMAN CD42b ANTIGEN

PRODUCT CODE : RDI-CBL 480   $375.00/vial
also available labeled to FITC: cat#RDI-CBL480FT   $375.00/100 Tests - DISCONTINUED

                                         PE: cat#RDI-CBL480PE   $469.00/100 Tests

CLONE: MM2/174

ISOTYPE: IgG1

SOURCE: Tissue culture supernatent.

PURIFICATION METHOD: Protein A affinity chromatography.

SPECIFICITY: MM2/174 immunoblots a band of 130kD on SDS gels of platelet lysates and gives surface immunofluorescence with fixed or unfixed platelets. It has been shown to react with GpIb by crossed-immunoelectrophoresis and by its lack of reaction with Bernard-Soulier platelets, either by flow cytometry or crossed-immunoelectrophoresis.


APPLICATIONS:

* Immunofluorescence studies of platelets, reacts with gp1b molecule.

* Studies with platelets and megakaryocytes in frozen sections by immunohistochemistry.

* Functional studies involving von Willebrands factor binding to gp1b.

Formalin fixed decalcified sections of bone marrow. Magakarocytes and platelets are labeled without pretreatment and with pretreatment using protease type XXIV (0.5mg/ml) High temp antigen retrieval citrate buffer pH6.0 may also be required

Flow cytometry


REFERENCES:

Thorsen, L. I. et al. Br. J. Haem. 68, 67 - 74 (1988)

Hornby, E.J. et al. Br. J. Haem. 79, 277 - 85 (1991)


PRESENTATION: Each monoclonal is delivered in a sealed vial at a concentration of  2 X 100ug (1mg/ml) in phosphate buffered saline solution containing 10mM sodium azide and 1mg/ml BSA. We recommend that each laboratory determine an optimum working titre for use in its particular application.

STORAGE: Purified monoclonal antibodies are generally stable for long periods at 4oC. However repeated warming to room temperature and re-cooling may result in a loss of activity and hence effective working titre. It is recommended that monoclonal antibodies are aliquotted upon receipt and then stored at -20C. After thawing they should then be used as a stock solution for up to 60 days. Dilute working solutions are best prepared on the day of use.

For research use only. Not supplied for use in human diagnostic or therapeutic procedures


DATA SHEET: mouse anti-human CD 42B clone HIP1

Catalog#: RDI-CD42BHIP1   $281.00/100ug  

Package Size: 100ug in 0.2ml, 0.5mg/ml) 50mM Tris-HCL (pH 8.0), 150mM NaCl, + 0.1% NaN3)

Species: mouse IgG1

Purification: purified from tissue culture supernatant by Protein G affinity chromatography

CLONE: HIP1

Workshop: IV, #P40

Activity: Reacts with a 135kd two chain membrane glycoprotein, GpIb, that forms a non covalent complex with GPIX (CD42a) found on platelets and magakaryocytes. Clone HIP1 antibody inhibits the ristocetin-dependent binding of von Willebrand Factor (vWF) to platelets, ristocetin-induced platelet agglutination and partially inhibits collagen induced aggregation. The GPIb/IX complex serves as the vWF surface receptor involved in the adhesion of platelets to the subendothelium of damaged vascular walls.

Application: -Indirect fluorescence

                     -adhesion (with azide free material)

                     -titers must be determined for each application|

Storage: Store at 4 Deg C .

Precautions: For In vitro research Use Only. Not for use in or on humans or animals or for diagnostics.

References: -Leucocyte Typing V, 1995 Oxford University Press, NY

-also available FITC labeled cat#RDI-CD42BHIP-FT $438.00/100T

-no azide formulation cat#RDI-CD42B-HIPX $750.00/0.5mg


RDI Division of Fitzgerald Industries Intl

34 Junction Square Drive

Concord MA 01742-3049

USA

phone (978) 371-6446 or (800) 370-2222

fax     (978) 371-2266

EMAIL:antibodies@fitzgerald-fii.com

Ordering terms

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