rev: September 2, 2004
HOME (index HOME page)
Return (master CD antibody index page)
CD CLUSTERED
ANTIBODIES
(anti-Human and others as indicated)
NOTE: Please inquire for bulk or custom formulations (without preservative or carrier).
RDI Division of Fitzgerald Industries Intl offers a wide line of antibodies. Since no one antibody works best for all applications (flow cytometry, neutralization, blotting, histochemistry, ELISA, etc), we offer many different types of antibodies to help solve this problem. Please inquire for other applications or types of antibodies not listed below. All products are for in vitro research use only-not for use in or on humans or animals-not for use in diagnostics. Price/availability/specifications subject to change without notice.
CD30
Antibodies
Mouse anti-human CD30 antibodies
-4 clones
CATALOG# CLONE# Workshop Host Form Price
RDI-CD30-BERH8 BERH8 IV mIgG1 purified $562.00
FITC $438.00/100t
RDI-CD30abm-G12 1G12 -- mIgG TCS $438.00/1ml (paraffin)
RDI-CD30abm-10 AC10 mIgG2b purified $312.00
(for CD30L=see CD153)
PRODUCT CODE :RDI-CBL482 $500.00/vial of 200 Tests
CLONE: Ki 1
ISOTYPE: IgG3
SOURCE: Tissue culture supernatant.
PURIFICATION METHOD: Not applicable
SPECIFICITY: Hodgkin and Reed-Sternberg cells of all histological sub-types
of Hodgkin's lymphomas. The CD30 antigen consists of two polypeptide chains,
mol.wt. 105 and 120 kDa
APPLICATIONS:
* Detection of Reed-Sternberg cells and especially mononuclear Hodgkin's cells.
* Suitable on cryostat sections and cell smears only.
REFERENCES: Froese,P. et al. J.Immunol 139, 2081 - 87 (1987)
Schwab,U.H. et al. Nature 299, 65 - 67 (1982)
Stein,H. et al. Int. J. Cancer 30, 445 - 59 (1982)
Ralfkiaer,E. et al. Arch Dermatol Res 279, 285 - 92 (1987)
PRESENTATION: Tissue culture supernatant dialysed against 0.05M Tris/HCl,
pH 7.2 containing sodium azide. Sufficient for 200 tests (1ml)
STORAGE: Purified monoclonal antibodies are generally stable for long periods
at 4oC. However repeated warming to room temperature and re-cooling may result
in a loss of activity and hence effective working titre. It is recommended
that monoclonal antibodies are aliquotted upon receipt and then stored at
-20C. After thawing they should then be used as a stock solution for up to
60 days. Dilute working solutions are best prepared on the day of use.
For research use only. Not supplied for use in human diagnostic or therapeutic procedures
Custom purified avaialble upon request.
Catalog#: RDI-CD30-BERH8 $562.00/vial
-also available FITC labeled: $438.00/100 Tests
cat#RDI-CD30-BER-FT
-
Biotin labeled: $594.00/0.5mg
cat#RDI-CD30-BER-BT
Package Size: 500ug purified in 50mM Tris-Hcl (pH 8.0), 0.15M NaCl and 0.1%
sodium azide. (sodium azide is toxic, dilute azide compounds with copious
amounts of water when disposing in drain to avoid accumulation of explosive
deposits-avoid contact with acids.)
Prep: purified from tissue culture supernatant via Protein G affinity
chromatography.
Species: mouse IgG1k
CLONE: BerH8
Workshop Code: Workshop IV, A105,
Activity: Reacts with a 120Kd type I transmembrane glycoprotein expressed
on in vitro stimulated T and B cells. CD30 is an activation marker which
was initially identified on Reed- Sternberg cells and is also expressed on
a few extrafollicular T and B cells located at the rim of germinal centers.
It is a cytokine receptor which belongs to the nerve growth factor receptor/tumor
necrosis factor receptor superfamily. CD30 interaction with CD30 Ligand (CD30L)
can mediate signals for proliferation, apotosis and cytotoxicity of lymphoid
cells. Clone BerH8 is suitable for staining formalin fixed paraffin embedded
tissue sections without pretreatment.
Application: -Indirect immunofluorescence cell surface staining
-histochemistry with paraffin sections
Titer for each application
References: 1) J. Immunol 151:5896
Storage: Store at 4 DEG C.
Precautions: For In vitro research Use Only. Not for use in or on humans
or animals or for diagnostics.
Catalog#: RDI-CD30abm-10 $312.00
Package Size: 100ug in 100ul (1mg/ml) 50mM sodium phosphate pH 7.5, 100mM
Potassium Chloride, 150mM NaCl, 0.5mg/ml Gentamicin Sulfate (preservative).
Species: mouse IgG1
Antigen: human YT lymphoma cells
Purity: >95% immunoglobulin by SDS-PAGE. Purified from low FBS containing
tissue culture supernatant using Protein A. product contains <1% bovine
immunoglobin.
CLONE: AC10
Activity: Reacts with human 30. Recognizes CD30 molecule of about 120kda.
Application: flow cytometry
-500K cultured CEM human tumor cells were washed and incubated 45minutes
on ice with 80ul of anti-CD30 antibody at 20ug/ml. Cells were washed twice
and incubated with secondary goat anti-mouse IgG:FITC, after which they were
washed three times, fixed and analyzed using a BD FACstar plus. Cells stained
positive with a mean shift of 0.39log10 fluorescent units when compared with
a mouse IgG2B negatove control. -titers must be determined for each
application
Storage: Store at 4 Deg C. DO NOT FREEZE.
Precautions: For In vitro research Use Only. Not for use in or on humansor
animals or for diagnostics.
References: -Blood 85:1-14 (1995), J Immunol 151:5896-5906 (1993)
Also available conjugated to PE: #RDI-CD30abm-10FT $469.00/120T
Biotin: cat#RDI-CD30abm-10BT $406.00/100ug
BULK Material Quoted on Request
It is the responsibility of the user to comply with all local/state and Federal rules in the use of this product. We are not responsible for any patent infringements that might result with the use of or derivation of this product.
34 Junction Square Drive
Concord MA 01742-3049
USA
phone (978) 371-6446 or (800) 370-2222
fax (978) 371-2266
EMAIL:antibodies@fitzgerald-fii.com
RETURN (CD Antibody INDEX page)