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CYTOKERATIN   ANTIBODIES  

(anti-Human and others as indicated)

RDI Divison of researchd Industries Intl  offers a wide line of  antibodies. Since no one antibody works best for all applications (neutralization, blotting, ELISA, etc), we offer many different types of antibodies to help solve this problem. Please inquire for other applications or types of antibodies not listed below.


ANTI-CYTOKERATIN  20


Mab to Cytokeratin 20

cat#RDI-PRO61033    $410.00/vial

Clone Determination      IT-Ks 20.5

Category                         Mouse monoclonal

Immunoglobulin Class    IgG2a

Purification                     Protein A affinity chromatograph

Antigen                            Electrophoretically purified cytokeratin (CK) 20 from human intestinal mucosa

Clinical Value                 IT-Ks 20.5 represents an excellent marker for certain types of carcinomas such as adeno-carcinomas of the colon, transitional cell carcinomas of the bladder and Merkel cell tumors of the skin. Very sensitive detection of intestinal and gastric fovelar epithelium, urothelial umbrella cells, Merkel cells of epidermis as metastatic colorectal carcinoma. Adenocarcinomas of breast, lung, endothelium and ovary (non-mucinous) as well as neuroendocrine tumors of the lung are essentially negative. Polypeptide recognized: protein IT (cytokeratin#20, Mr 46,000)

Application                       Suitable for frozen tissue sections and cytological material

                                          Immunoblotting (Western)

Stability/Storage at 2-8°C one year after reconstitution when stored at 2-8 DEG C. For extended storage, keep in aliquots at -20  DEG C. Avoid repeated thawing and freezing.

Quantity                              50 µg (lyoph.) from PBS which contains 0.5%BSA and 0.09% NaN3

Reconstitution:                  Add 1ml DI Water

Polypeptide Reacting       Protein IT (cytokeratin No. 20; Mr 46 000)

Antigen Recognized in     Human (NOT with rat)

Reactivities on Cultured cell lines tested so far:

                     HT-29, LoVo, DLD-1, SW 1116, Cell Lines ,CaCo-2, RT-4

Working Dilution    Reconstitute in 1 ml dist. water and dilute 1:10 in PBS (pH 7.4) for immunohistochemical application.

Incubation Time 1 h at RT or over night at 4°C.

Literature on Cytokeratin 20

Moll, R., Löwe, A., Laufer, J. and Franke, W.W.: Cytokeratin 20 in human carcinomas: A new histodiagnostic marker detected by monoclonal antibodies. Am.J.Path. 140, 427-447 (1992)

Moll, R., Schiller, D.L. and Franke, W.W.: Identification of protein IT of the intestinal cytoskeleton as a novel type I cytokeratin with unusual properties and expression patterns. J.Cell.Biol. 111, 567-580 (1990)

Moll, I. and Moll, R.: Comparative cytokeratin analysis of sweat gland ducts and eccrine poromas. Arch Dermatol Res. 283, 300-309 (1991)

Moll, R., Zimbelmann, R., Goldschmidt, M.D., Keith, M., Laufer, J., Kasper, M., Koch, P.J., Franke, W.W.: The human gene encoding cytokeratin 20 and its expression during fetal development and in gastrointestinal carcinomas. Differentiation 53, 75-93 (1993)

V.J. Möbus, R. Moll, C.D. Gerharz, D.G. Kieback, W. Weikel, G. Hoffmann & R. Kreienberg: Establishment of new ovarian and colon carcinoma cell lines: differentiation is only by cytokeratin analysis. Br. J. Cancer 69, 422-428 (1994)

For research use only. Not supplied for use in human diagnostic or therapeutic procedures


-see also rec human cytokeratin 20 antigen


RDI Divison of researchd Industries Intl

San Jose, 95123 CA Snell ave 658

USA

or (800) 370-2222

EMAIL:margaret@cellular-research.com

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