RDI Divison of researchd Industries Intl offers a wide line of antibodies. Since no one antibody works best for all applications (neutralization, blotting, ELISA, etc), we offer many different types of antibodies to help solve this problem. Please inquire for other applications or types of antibodies not listed below.

ANTI-CYTOKERATIN 20

cat#RDI-PRO61033 $410.00/vial

Clone Determination IT-Ks 20.5

Category Mouse monoclonal

Immunoglobulin Class IgG2a

Purification Protein A affinity chromatograph

Antigen Electrophoretically purified cytokeratin (CK) 20 from human intestinal mucosa

Clinical Value IT-Ks 20.5 represents an excellent marker for certain types of carcinomas such as adeno-carcinomas of the colon, transitional cell carcinomas of the bladder and Merkel cell tumors of the skin. Very sensitive detection of intestinal and gastric fovelar epithelium, urothelial umbrella cells, Merkel cells of epidermis as metastatic colorectal carcinoma. Adenocarcinomas of breast, lung, endothelium and ovary (non-mucinous) as well as neuroendocrine tumors of the lung are essentially negative. Polypeptide recognized: protein IT (cytokeratin#20, Mr 46,000)

Application Suitable for frozen tissue sections and cytological material

Immunoblotting (Western)

Stability/Storage at 2-8°C one year after reconstitution when stored at 2-8 DEG C. For extended storage, keep in aliquots at -20 DEG C. Avoid repeated thawing and freezing.

Quantity 50 µg (lyoph.) from PBS which contains 0.5%BSA and 0.09% NaN3

Reconstitution: Add 1ml DI Water

Polypeptide Reacting Protein IT (cytokeratin No. 20; Mr 46 000)

Antigen Recognized in Human (NOT with rat)

Reactivities on Cultured cell lines tested so far:

HT-29, LoVo, DLD-1, SW 1116, Cell Lines ,CaCo-2, RT-4

Working Dilution Reconstitute in 1 ml dist. water and dilute 1:10 in PBS (pH 7.4) for immunohistochemical application.

Incubation Time 1 h at RT or over night at 4°C.

Literature on Cytokeratin 20

Moll, R., Löwe, A., Laufer, J. and Franke, W.W.: Cytokeratin 20 in human carcinomas: A new histodiagnostic marker detected by monoclonal antibodies. Am.J.Path. 140, 427-447 (1992)

Moll, R., Schiller, D.L. and Franke, W.W.: Identification of protein IT of the intestinal cytoskeleton as a novel type I cytokeratin with unusual properties and expression patterns. J.Cell.Biol. 111, 567-580 (1990)

Moll, I. and Moll, R.: Comparative cytokeratin analysis of sweat gland ducts and eccrine poromas. Arch Dermatol Res. 283, 300-309 (1991)

Moll, R., Zimbelmann, R., Goldschmidt, M.D., Keith, M., Laufer, J., Kasper, M., Koch, P.J., Franke, W.W.: The human gene encoding cytokeratin 20 and its expression during fetal development and in gastrointestinal carcinomas. Differentiation 53, 75-93 (1993)

V.J. Möbus, R. Moll, C.D. Gerharz, D.G. Kieback, W. Weikel, G. Hoffmann & R. Kreienberg: Establishment of new ovarian and colon carcinoma cell lines: differentiation is only by cytokeratin analysis. Br. J. Cancer 69, 422-428 (1994).