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CYTOKERATIN ANTIBODIES
(anti-Human and others as indicated)
RDI Division of Fitzgerald Industries Intl offers a wide line of antibodies. Since no one antibody works best for all applications (neutralization, blotting, ELISA, etc), we offer many different types of antibodies to help solve this problem. Please inquire for other applications or types of antibodies not listed below.
ANTI-CYTOKERATIN TYPE 18
Mab to Human Cytokeratin 18
cat#RDI-PRO61028, new cat#: 10R-C161a $412.50/vial
also available Biotin conjugated: cat#RDI-PRO61528, new cat#: 61R-C161aBT $517.00/vial 0.25ml
FITC conjugated: cat#RDI-PRO61428, new cat#: 61R-C161aFT $517.00/0.25ml
Clone Determination Ks 18.04
Category Mouse monoclonal
Immunoglobulin Class IgG1
Purification Protein A (recombinant)
Antigen Human Cytokeratin 18 from HeLa cytoskeletal preparation
Specificity Ks 18.04 represents an excellent marker to discriminate simple epithelia from those of different origin
Application Suitable for frozen and parrafin embeded tissue Cytological material
Immunoblotting (Western)
(micorwave release recommended for paraffin)
Storage At 2-8 DEG C one year after reconstitution
Quantity 50 µg (lyoph.) ; contains 0.5% BSA and 0.09% NaN3
Polypeptide Reacting Mr 45 000 polypeptide ( human cytokeratin 18) of all simple type epithelia and basal cells of many squamous, nonepidermal epithelia
Antigen Recognized in Human, mouse, rat, bovine, pig, dog,
Species (tested so far) hamster, sheep, fish (trout)
Reactivities on Cultured HeLa and MCF-7
Cell Lines (tested so far)
Tumors Specifically Detected All tested adeno Ca.; mammary CA, urinary bladder CA, undiff CA, cervix CA,
h epatocellular CA.
Working Dilution After reconstitution with 1 ml dist. water, dilute 1:20 with PBS, pH 7.4 for use in
i mmunohistochemistry
Incubation Time 1 h at 37 DEG C (extended with paraffin (suggest overnight at 2-8 DEG C)
References:
Bartek,J., Vojtesek,B., Staskova,Z., Bartkova,J.,Kerekes,Z.
Rejthar,A. and Kovarik,J.: A series of 14 new monoclonal antibodies to
keratins: Characterization and value in diagnostic histopathology.J. Pathol.(1991) in press.
Heid,H., Vojtesek,B., Bruder,G., Kaufman, M.,
Stackova,Z., Kovarik,J.,and Franke W.W.: Cell type heterogeneity of mammary
gland epithelial and breast carcinoma cells as revealed by cytokeratin
expression. Differentiation (1991), submitted.
Layerova,L.,Kovarik,J.,Bartek,J.,Rejthar,A. and
Vojtesk, B.: Novel monoclonal antibodies defining epitope of human cytokeratin
18 molecule. Hybridoma 7, 495-504 (1988).
Markl,J., Winter,S. and Franke,W.W." The catalog
and the expression complexity oof cytokeratins in a lower verte-brate:
Biochemical identification of cytokerations in a teleost fish, the rainbow
trout. Eur.J.Cell Ciol.50, 1-16 (1989).
Moll,R., Franke, W.W., Schiller, D.L.,Geiger, B. and
Krepler, R.: The catalog of human cytokeratins: Patterns of expression in
normal epithelia, tumors and cultured cells 31, 11-24 (1982).
Gomi,H.,Yokoyama,T., Fujmoto,K., Ikeda,T., Katoh,A.,Itoh, T, and Itohara,S.:
Mice Devoid of the Glial Fibrillary Acidic Protein Develop Normally and
Are Susceptible to Scrapie Prions. Neuron,Vol.
14,29-41 (1995).
also available as 5ml TCS cat#RDI-PRO65028 $219.00/vial
For Research Use Only
RDI Division of Fitzgerald Industries Intl
phone (978) 371-6446 or (800) 370-2222
fax (978) 371-2266
EMAIL:antibodies@fitzgerald-fii.com
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