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CYTOKERATIN   ANTIBODIES  

(anti-Human and others as indicated)

RDI Divison of researchd Industries Intl  offers a wide line of  antibodies. Since no one antibody works best for all applications (neutralization, blotting, ELISA, etc), we offer many different types of antibodies to help solve this problem. Please inquire for other applications or types of antibodies not listed below.


ANTI-CYTOKERATIN 7


Mab to Cytokeratin 7     cat#RDI-PRO61025   $375.00/50ug


Clone Determination                   Ks 7.18

Category                                    Mouse monoclonal

Immunoglobulin Class                 IgG1

Purification                                 SpAss Protein A (recombinant)

Antigen                                      Cytoskeletal proteins from cultured HeLa cells

Special Note                             Ks 7.18 represents an excellent marker for the discrimination of specific

                                                 subtypes of    adenocarcinoma: Eg adeno-CA of pancreas, bile duct

                                                 CA and transitional CA of bladder are positively stained, whereas colorectal,

                                                 hepatocellular and prostate CA are negative

Application                               Suitable for frozen and paraffin embedded tissue Cytological material

Stability/Storage                      One year after reconstitution when stored at 4°C

Quantity                                 50µg (lyoph.)

Polypeptide Reacting             MR 54 000 polypeptide (cytokeratin no.7) of human glandular epithelia

Antigen Recognized in Species

(tested so far)                        Human, bovine, pig, sheep; not with dog, rabbit, rat, mouse

Reactivities on Cultured

Cell Lines (tested so far)       HeLa, RT 112, T24, BT-20, CAMA-1 Detroit 562, (MCF-7, HT-29, are negative)

Tumors Specifically Detected Specific subtypes of adeno-CA: Adeno-CA of pancreas, gallbladder, lung, cervix; cholangio-CA of liver; ductal and lobular CA of breast; CA of ovary; transitional cell CA of bladder; mesothelioma; negative with colorectal Adeno CA and most cases of hepatocellular CA. Occasionally, staining of blood vessel walls, particularly of endothelial cells may be observed.

Application Suitable for frozen and paraffin (P) embedded tissue ,Cytological material Pretreatment With paraffin embedded sections, protease pretreatment is required prior to antibody application Working Dilution When reconstituted with 1ml destilled water, dilute 1:50 (at least) with PBS, pH 7.4 for use in immunohistochemistry Incubation Time 1h at RT, extended with P (over night at 4°C)


References

Bartek J., Vojtesek B., Staskova Z., Bartkova J., Kerekes Z., Rejthar A. and Kovarik J.: A series of 14 new monoclonal antibodies to keratins: Characterization and value in diagnostic histopathology. J. Pathol., 164, 215-224 (1991)

Lauerova,L., Kovarik, J., Bartek, J., Reithar, A. and Vojtesek, B.: novel monoclonal antibodies defining epitope of human cytokeratin 18 molecule. Hybridoma 7, 495-504 (1988)

Osborn, M., van Lessen, G., Weber, K. Klöppel, G. and Altmannsberger, M.: Methods in Laboratory Investigation. Differential diagnosis of gastrointestinal carcinomas by using monoclonal antibodies specific for individual keratin polypeptides. Lab. Investigation 55,4, 497-504 (1986)

Ramaekers, F., van Niekerk C., Poels, L., Schaafsma, E., Huijsmans, A., Robben, H., Schaart, G. and Vooijs, P.: Use of monoclonal antibodies to keratin 7 in the differential diagnosis of adenocarcinomas. Am. J. Pathol. 136, 641-655 (1990)

Stosiek, P. and Kasper, M.: Neoexpression von Cytokeratin 7 bei der chronisch-atrophischen Gastritis mit perniziöser Anämie. Pathologe 11, 14-17 (1990)

Vojtesek, B., Stasková, Z., Nenutil, R., Bártková, J., Kovarik, J., Rejthar, A. and Bártek, J.: A panel of monoclonal antibodies to keratin No. 7: Characterization and value in tumor diagnosis. Neoplasma 37, 333-342 (1989)




For research use only. Not supplied for use in human diagnostic or therapeutic procedures


RDI Divison of researchd Industries Intl

San Jose, 95123 CA Snell ave 658

USA

or (800) 370-2222

EMAIL:margaret@cellular-research.com

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