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                                 ANTIBODIES  

(anti-Human and others as indicated)

RDI Divison of Fitzgerald Industries Intl  offers a wide line of  antibodies. Since no one antibody works best for all applications (neutralization, blotting, histochemistry, ELISA, etc), we offer many different types of antibodies to help solve this problem. Please inquire for other applications or types of antibodies not listed below.


ANTI-Elastin (and related, tropoelastin....)  inquire for fibrillin abs
Rabbit ANTIBODY TO ELASTINS FROM SEVERAL SPECIES

cat#RDI-TP592    $344.00/1ml    

DESCRIPTION Polyclonal antibody to elastins from several species.

-SPECIFICITY Tested by ELISA, Western blot and immunohistology techniques. Shown to cross react with elastins from the following species in decreasing order of reactivity: Pig, Human, Dog, Chick, Rat, and low reactivity for Cow. SUGGESTED WORKING DILUTIONS Different assay conditions require that serial dilution of all reagents be performed to determine optimal working concentrations. Prepare working dilution immediately before use. Storage at a working dilution may result in performance loss. Suggesting starting dilutions are as follows: Titer ca. 1:4000 for human elastin Store at 4°C until rehydrated. Stable for a minimum of 1 year at 4°C. No preservatives added. Additional biological protection may be provided with 0.01% thimerosal. Non-Sterile. REHYDRATION and STORAGE: Sample may be diluted directly into phosphate buffered saline (PBS), neutral pH buffer, or H2O., However, dilution with 50% glycerol is preferred. This procedure eliminates freezing at -20°C and 50% glycerol is an effective biological inhibitor when the product is stored at 4°C. At most working dilutions, the level of glycerol is too small to affect most assays. Note: Variations in temperature due to freeze cycles may cause loss of activity when rehydrated product is stored frozen in aliquots less that 50µl.


Polyclonal antiserum to bovine ligamentun Nuchae Elastin..

--cat#RDI-PR403   $344.00/0.5ml vial

-Whole serum obtained from rabbits immunized with the alpha-elastin fraction of insoluble elastin purified by hot sodium hydroxide from bovine ligamentum nuchae. The antibody reacts most strongly with bovine insoluble elastin and less strongly with bovine tropoelastin. It does show cross-reactivity with elastin from other species, although reactivity is lower for non-bovine elastins. Those elastins for which reactivity has been documented include human, rabbit, pig, sheep and dog. This antibody show  little reactivity with rat or mouse elastin.


Polyclonal, antiserum to human aortic alpha-elastin.

--cat#RDI-PR533  $344.00/0.5ml vial  

Whole serum obtained from rabbits immunized with the alpha-elastin fraction of insoluble elastin purified by hot sodium hydroxide from human aorta. Tested by immunohistology as well as ELISA and Western blot techniques, this product reacts most strongly with human insoluble elastin and human tropoelastin. It does show cross-reactivity with elastin from other species, although reactivity is much lower for non-human elastins. Those elastins for which reactivity has been documented include bovine,rabbit,sheep and dog. This antibody shows little if any reactivity with rat elastin.


Polyclonal antisrum to bovine tropoelastin.

--cat#RDI-PR396  $500.00/0.5ml

-Whole serum obtained from rabbits immunized with tropoelastin purified from fetal bovine ligamentum nuchae. This product reacts most strongly with bovine tropoelastin and less strongly with insoluble forms of elastin. It does show extensive cross-reactivity with elastin from other species, although reactivity is somewhat lower for non-bovine elastins. Those elastins for which reactivity has been documented include human, rabbit,pig,sheep, dog and rat.


Polyclonal (rabbit) antiserum to Human tropoelastin.

--cat#RDI-PR398   $500.00/0.5ml

Whole serum obtained from rabbits immunized with recombinant human tropoelastin. Tested by immunohistology as well as ELISA and western blot techniques. This product reacts most strongly with human tropoelastin and less strongly with insoluble forms of elastin. It does show extensive cross-reactivity with elastin from other species, although reactivity is somewhat lower for non-human elastins. Those elastins for which reactivity has been documented include bovine, rabbit,pig,sheep, and dog rat.


Polyclonal (rabbit) antiserum to tropoelastin.

--cat#RDI-GA317   $500.00/1.0ml  

Antigen:ALA-LYS-ALA-ALA-ALA-LYS-ALA-ALA-ALA-LYS-Tyr peptide covalently linked to thyroglobulin. Represents a repeat sequence of elastin that becomes desmosine corsslinks. Is not present in mature, cross linked elastin.

-supplied as 1ml lyophilized whole serum. No species specificity. approx 1:100 for immunohoistochemistry, 1:2K for microtiter plate assays.


Monoclonal antiserum to bovine tropoelastin.

--cat#RDI-MM436   $375.00/0.2ml  

Whole sascites fluid from mice immunized wth a hybridoma producing antibody to bovive ligamentum nuchae tropoelastin. The epitope is unknown. Tested by immunohistology as well as ELISA and western blot techniques. This product reacts most strongly with bovine tropoelastin and insoluble forms of elastin. It does show limited croos-reactivity with elastin from other species, although reactivity is somewhat lower for non-bovine elastins. Those elastins for which reactivity has been documented include human, rabbit, pig, and sheep. Little to no cross with rat or chicken .


Rabbit anti-mouse tropoelastin (exons 6-17).

cat#RDI-PR385  $438.00/vial 0.5ml

Whole serum obtained from rabbits immunized with a bacterial fusion protein

containing protein encoded by exons 6-17 of mouse tropoelastin. the fusion protein

also containied a His-Tag sequence. Tested by immunohistology as well as ELISA and Western blot. Reactivity with tropoelastin from other species has not been determined.


Rabbit anti-mouse tropoelastin (exons 31-36).

cat#RDI-PR387  $438.00/vial 0.5ml

Whole serum obtained from rabbits immunized with a bacterial fusion protein containing protein encoded by exons 31-36 of mouse tropoelastin. The fusion protein also containied a His-Tag sequence. Tested by immunohistology as well as ELISA and Western blot. Reactivity with tropoelastin from other species has not been determined.


Goat anti-RAT LUNG ALPHA-ELASTIN

cat#RDI-RA75   $312.00/0.5ml vial  

Antigen: Alpha-elastin from rat lung covalently linked to keyhole limpets hemocyanin. For Use in ELISA.


Goat anti-rat alpha-elastin.

cat#RDI-RT675     sold out


Rabbit anti-alpha-elastin

cat#RDI-L986    $250.00/0.5ml vial

Antigen: Alpha-elastin from bovine neck ligament. Host: Rabbit

Cross-reactivity: Insoluble and alpha-elastin from human, rabbit, porcine and hamster. Does not cross-react with chicken or rat  elastins.

Suitable for ELISA techniques. Does not work well with Western blot.


old cat#RDI-PR590 sold out Feb 2001

MONOCLONAL ANTISERA, anti-elastin

cat#RDI-MM872   $375.00/0.2ml    

Ascities fluid obtained from mice immunized with a hybridoma-(clone BA4) producing antibody to bovine alpha-elastin. The epitope has been mapped to a VGVAPG repeat in elastin. The antibody type is IgG1 and reacts with protein A. Tested by immunohistology as well as ELISA and Western blot techniques, this product reacts most strongly with bovine tropoelastin and insoluble forms of elastin. It does show cross reactivity with elastin from other species, although reactivity is much lower for non-bovine elastins. Those elastins for which reactivity has been documented include human,rabbit,pig,goat,cat,guinea and sheep. This antibody shows little reactivity towards rat or chicken elastins.


MONOCLONAL, anti-elastin

cat#RDI-MM436  $375.00/0.2ml   

Whole ascities fluid from mice immunized with a hybridoma-producing antibody to bovine ligamentum nuchae tropoelastin. The epitope on tropoelastin is uncharacterized. This product reacts most strongly with bovine tropoelastin and insoluble forms of elastin. It does show limited cross-reactivity with elastin from other species, although reactivity is lower for non-bovine elastins. Those elastin for which reactivity has been documented include human,rabbit,pig and sheep. This antibody show little if any reactivity towards rat or chicken elastins.


GENERAL INFORMATION ABOUT ANTIBODIES

FORM/STORAGE

All antibodies are sent lyophilized. Store at 4'C until rehydrated. Stable for a minimum of 1 year at 4'C. No preservatives have been added. Additional biological protection may be provided with 0.01% thimerosal. Do not use sodium azide if antibody is to be used with peroxidase. Non-sterile.


REHYDRATION AND STORAGE:

Two methods for rehydration and storage are recommended to meet needs. Procedure A using 50% glycerol is preferred. It eliminates freezing at -20'C and 50% glycerol is an effective biological inhibitor when the product is stored at 4'C. At most working dilutions, the level of glycerol is to small to affect most assays.


Procedure A

Mis 1 ml glycerol with 1 ml of reagent quality water. Transfer 1 ml of this 50% glycerol solution to the product vial. Rotate the vial until the lyophilized pellet is dissolved. Dilute to desired concentration with PBS or other buffer. This product may be stored either refrigerated or frozen as desired. Product is stable for minimum of 1 year.

Procedure B

Rehydrate with 1 ml of reagent quality water. Dilute to desired concentration with PBS or other buffer. This product may be stored for up to 1 week refrigerated; thereafter, it should be stored frozen. When frozen, product is stable for a minimum of 1 year.

Note: Variations in temperature due to freeze cycles may cause loss of activity when rehydrated product is stored frozen in aliquots less than 50 ul.

SUGGESTED WORKING DILUTIONS:

Different assay conditions require that serial dilution of all  reagents be performed to determine optimal working concentrations Prepare working dilution immediately before use. Storage at a working dilution may result in performance loss. Unless otherwiseindicated, suggesting starting dilutions for all antibodies are as follows:

Microwell Plate Assays: 1:500-1:2000

Western blots   1:200-1:450

Immunoprecipitation 1:100-1:200

Histo/Cytochemistry 1:50-1:100

Immunocytochemistry 1:50-1:100

-copyright 1997 by owner


RDI Divison of Fitzgerald Industries Intl

34 Junction Square Drive

Concord MA 01742-3049

USA

phone (978) 371-6446 or (800) 370-2222

fax     (978) 371-2266

EMAIL:antibodies@fitzgerald-fii.com

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