rev: 5/15/2008
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ANTIBODIES
(anti-Human and others as indicated)
RDI Division of Fitzgerald Industries Intl offers a wide line of antibodies. Since no one antibody works best for all applications (neutralization, blotting, histochemistry, ELISA, etc), we offer many different types of antibodies to help solve this problem. Please inquire for other applications or types of antibodies not listed below.
see below monoclonal and polyclonal against Calbindin
see also pure protein at bottom of page
Monoclonal anti-calbindin D-28K.
CAT#: RDI-CALBINDabm $484.00/vial
LOT#:
Form: Lyophilized ascites (no preservatives).
Quantity: 200 ul.
Reconstitution: with 200 ul bidistilled
water.
Description: Monoclonal anti-calbindin D-28k is a
mouse IgG produced by hybridization of mouse myeloma
cells with spleen cells from mice immunized with calbindin
D-28k purified from chicken gut. The antibody was evaluated for specificity and
potency:a) by indirect immunofluorescent
or immunoperoxidase labelling
as well as PAP and Biotin-Avidin labeling of cryostat
or vibratome-sections of 4% paraformaldehyde
fixed tissue; b) by immunoenzymatic labeling of immunoblots; c) by radioimmunoassay.
The product is a monoclonal antibody (McAB) against calbindin D-28k, and calcium-binding protein of the EF-
hand family related to calmodulin and troponin-C. The antibody reacts specifically with calbindin D-28k in tissue originating from human, monkey,
rabbit, rat, mouse and chicken (but probably not fish). This antibody does not
cross-react with calretinin or other known calcium
binding-proteins. This McAB specifically stains the
45Ca-binding spot of calbindin D-28k (MW 28'000. IEP 4.8) in a two-dimensional gel. In radioimmunoassay it
detects calbindin D-28k with a sensitivity of 10 ng/assay and an affinity of 1.6 x 10(12) L/M.
Dilution: -Immunohistochemistry: 1:5000 - 1:10,000
with avidin- biotin methods.
-Immunoblots: 1:100 - 1:1,000.
We recommend that the optimal dilutions be determined by titration experiments.
Storage: For long storage, keep at -80'C (or at least -20'C). For continuous use keep at 4'C (with 0.01% Na-azide).
Avoid repeated freezing and thawing.
References: 1. Celio M.R. et al., Cell Calcium
11:599-602, 1990.
-For Research use Only
Rabbit anti-Calbindin D-28k [NEW BATCH]
CAT#: RDI-Calbindabr $517.00/vial
Form: Lyophilized antiserum (no preservatives).
Quantity: 200 ul.
Reconstitution: with 200 ul bidistilled
water.
Description: This antiserum was produced against recombinant rat calbindin D-28k. It cross reacts
with many other species, including humans. It can be used for immunohistochemistry and immunoblotting.
In immunoblots it has 10% crossreaction
with calretinin. Thus, depending on the dilution of
the antiserum and/or sensitivity of the immunostaining,
this other calcium- binding protein could be also revealed. In case of doubt,
we suggest to use our monoclonal antibody cat#RDI-CALBINDabm
directed against calbindin D-28k, which does not
recognize calretinin.
dilutions: Immunohistochemistry: 1:10,000 - 1:20,000 with the avidin-biotin methods.
Immunoblots: 1:100 - 1: 1,000
We recommend that the optimal dilutions be determined by titration
experiments.
Storage: Reconstitute by shaking very long and sucking with a Pasteur-pipette
until the pellet is dissolved. Make small portions (e.g.2-5 ul).
For long storage, keep at -80'C (or at least -20'C). For
continuous use keep at 4'C (with 0.01% Na-azide).
Avoid repeated freezing and thawing.
For Research Use Only
Rabbit
antibody to Calbindin D-9k.
CAT#: RDI-RTCALD9Kabr, new cat#: 20R-CR004 $517.00/200ul
Form: Lyophilized antiserum
Quantity: 200 ul
Reconstitution: with 200 ul bidistilled
water.
Description: This antibody was produced against rat recombinant Calbindin D-9k. It can be utilized in immunohistochemistry
and in immunoblots.
Dilutions: Immunohistochemistry: 1:1,000 - 1: 10,000
with the avidin-biotin method.
Immunoblots: 1: 1,000.
We recommend that the optimal dilutions be determined by titration experiments.
Storage: For long storage, keep at -80'C (or at least -20'C). For continuous use keep at 4'C (with 0.01% Na-azide).
Avoid repeated freezing and thawing.
For Research Use Only
Calbindin-D9k (porcine intestine)
cat#RDI-CALBD9K-AG $500.00/200ug $438.00/vial 3+
White lyophilized solid. Intestinal vitamin D-dependent calcium-binding protein. Functions as a cytosolic buffer for Ca2+ ions in duodenal enterocytes and facilitates transepithelial active transport of Ca2+ ions. Also present in porcine uterus and placenta. Binds to two equivalents of Ca2+. Purity: >98% by HPLC. Sold on the basis of peptide content. Molecular Weight: 8840.0 Solubility: H2O Storage: Freezer (-20°C). This product is stable for 2 years as supplied.
Ref.: Schroder, B., et al. 1996. J. Physiol. 492, 715; Hitchman,
A.J., and
Recombinant rat calbindin D-9K
cat#RDI-RTCALBD9k-AG $312.00/10ug $1000.00/60ug
Recombinant rat calbindin D-28K
cat#RDI-RTCALBD28K-AG, new cat#: 30R-AC003 $313.50/10ug
RDI Division of Fitzgerald Industries Intl
phone (978) 371-6446 or (800) 370-2222
fax (978) 371-2266
EMAIL:antibodies@fitzgerald-fii.com
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