rev: May 20, 2003
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ANTIBODIES
(anti-Human and others as indicated)
RDI Divison of Fitzgerald Industries Intl offers a wide line of antibodies. Since no one antibody works best for all applications (neutralization, blotting, histochemistry, ELISA, etc), we offer many different types of antibodies to help solve this problem. Please inquire for other applications or types of antibodies not listed below.
see below monoclonal and polyclonal against Calbindin
see also pure protein at bottom of page
CAT#: RDI-CALBINDabm $438.00/vial
LOT#:
Form: Lyophilized ascites (no preservatives).
Quantity: 200 ul.
Reconstitution: with 200 ul bidistilled water.
Description: Monoclonal anti-calbindin D-28k is a mouse IgG produced by
hybridization of mouse myeloma cells with spleen cells from mice immunized
with calbindin D-28k purified from chicken gut. The antibody was evaluated
for specificity and potency:a) by indirect immunofluorescent or immunoperoxidase
labelling as well as PAP and Biotin-Avidin labeling of cryostat or
vibratome-sections of 4% paraformaldehyde fixed tissue; b) by immunoenzymatic
labeling of immunoblots; c) by radioimmunoassay.
The product is a monoclonal antibody (McAB) against calbindin D-28k, and
calcium-binding protein of the EF- hand family related to calmodulin and
troponin-C. The antibody reacts specifically with calbindin D-28k in tissue
originating from human, monkey, rabbit, rat, mouse and chicken (but probably
not fish). This antibody does not cross-react with calretinin or other known
calcium binding-proteins. This McAB specifically stains the 45Ca-binding
spot of calbindin D-28k (MW 28'000. IEP 4.8) in a two-dimensional gel. In
radioimmunoassay it detects calbindin D-28k with a sensitivity of 10 ng/assay
and an affinity of 1.6 x 10(12) L/M.
Dilution: -Immunohistochemistry: 1:5000 - 1:10,000 with avidin- biotin methods.
-Immunoblots: 1:100 - 1:1,000.
We recommend that the optimal dilutions be determined by titration experiments.
Storage: For long storage, keep at -80'C (or at least -20'C). For
continuous use keep at 4'C (with 0.01% Na-azide). Avoid repeated freezing
and thawing.
References: 1. Celio M.R. et al., Cell Calcium 11:599-602, 1990.
-For Research use Only
dilutions: Immunohistochemistry: 1:10,000 - 1:20,000 with the avidin-biotin methods.
Immunoblots: 1:100 - 1: 1,000
We recommend that the optimal dilutions be determined by titration
experiments.
Storage: Reconstitute by shaking very long and sucking with a Pasteur-pipette
until the pellet is dissolved. Make small portions (e.g.2-5 ul). For long
storage, keep at -80'C (or at least -20'C). For continuous use keep at 4'C
(with 0.01% Na-azide). Avoid repeated freezing and thawing.
For Research Use Only
LOT#:
Form: Lyophilized antiserum
Quantity: 200 ul
Reconstitution: with 200 ul bidistilled water.
Description: This antibody was produced against rat recombinant Calbindin
D-9k. It can be utilized in immunohistochemistry and in immunoblots.
Dilutions: Immunohistochemistry: 1:1,000 - 1: 10,000 with the avidin-biotin
method.
Immunoblots: 1: 1,000.
We recommend that the optimal dilutions be determined by titration experiments.
Storage: For long storage, keep at -80'C (or at least -20'C). For continuous
use keep at 4'C (with 0.01% Na-azide). Avoid repeated freezing and thawing.
For Research Use Only
cat#RDI-CALBD9K-AG $500.00/200ug $438.00/vial 3+
White lyophilized solid. Intestinal vitamin D-dependent calcium-binding protein. Functions as a cytosolic buffer for Ca2+ ions in duodenal enterocytes and facilitates transepithelial active transport of Ca2+ ions. Also present in porcine uterus and placenta. Binds to two equivalents of Ca2+. Purity: >98% by HPLC. Sold on the basis of peptide content. Molecular Weight: 8840.0 Solubility: H2O Storage: Freezer (-20°C). This product is stable for 2 years as supplied.
Ref.: Schroder, B., et al. 1996. J. Physiol. 492, 715; Hitchman, A.J., and Harrison, J.E. 1972. Can. J. Biochem. 50, 758.
cat#RDI-RTCALBD9k-AG $312.00/10ug $1000.00/60ug
cat#RDI-RTCALBD28K-AG $281.00/10ug $1000.00/100ug
RDI Divison of Fitzgerald Industries Intl
34 Junction Square Drive
Concord MA 01742-3049
USA
phone (978) 371-6446 or (800) 370-2222
fax (978) 371-2266
EMAIL:antibodies@fitzgerald-fii.com
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