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ANTIBODIES  

(anti-Human and others as indicated)

RDI Divison of Fitzgerald Industries Intl  offers a wide line of  antibodies. Since no one antibody works best for all applications (neutralization, blotting, histochemistry, ELISA, etc), we offer many different types of antibodies to help solve this problem. Please inquire for other applications or types of antibodies not listed below.


see below monoclonal and polyclonal against Calbindin

see also pure protein at bottom of page


Monoclonal anti-calbindin D-28K.

CAT#: RDI-CALBINDabm     $438.00/vial

LOT#:

Form: Lyophilized ascites (no preservatives).

Quantity: 200 ul.

Reconstitution: with 200 ul bidistilled water.

Description: Monoclonal anti-calbindin D-28k is a mouse IgG produced by hybridization of mouse myeloma cells with spleen cells from mice immunized with calbindin D-28k purified from chicken gut. The antibody was evaluated for specificity and potency:a) by indirect immunofluorescent or immunoperoxidase labelling as well as PAP and Biotin-Avidin labeling of cryostat or vibratome-sections of 4% paraformaldehyde fixed tissue; b) by immunoenzymatic labeling of immunoblots; c) by radioimmunoassay.

The product is a monoclonal antibody (McAB) against calbindin D-28k, and calcium-binding protein of the EF- hand family related to calmodulin and troponin-C. The antibody reacts specifically with calbindin D-28k in tissue originating from human, monkey, rabbit, rat, mouse and chicken (but probably not fish). This antibody does not cross-react with calretinin or other known calcium binding-proteins. This McAB specifically stains the 45Ca-binding spot of calbindin D-28k (MW 28'000. IEP 4.8) in a two-dimensional gel. In radioimmunoassay it detects calbindin D-28k with a sensitivity of 10 ng/assay and an affinity of 1.6 x 10(12) L/M.

Dilution: -Immunohistochemistry: 1:5000 - 1:10,000 with avidin- biotin methods.

               -Immunoblots: 1:100 - 1:1,000.

We recommend that the optimal dilutions be determined by titration experiments.

Storage: For long storage, keep at -80'C (or at least -20'C).   For continuous use keep at 4'C (with 0.01% Na-azide).   Avoid repeated freezing and thawing.

References: 1. Celio M.R. et al., Cell Calcium 11:599-602, 1990.

-For Research use Only


Rabbit anti-Calbindin D-28k [NEW BATCH]

CAT#: RDI-Calbindabr    $470.00/vial

LOT#:

Form: Lyophilized antiserum (no preservatives).

Quantity: 200 ul.

Reconstitution: with 200 ul bidistilled water.

Description: This antiserum was produced against recombinant rat calbindin D-28k. It cross reacts with many other species, including humans. It can be used for immunohistochemistry and immunoblotting. In immunoblots it has 10% crossreaction with calretinin. Thus, depending on the dilution of the antiserum and/or sensitivity of the immunostaining, this other calcium- binding protein could be also revealed. In case of doubt, we suggest to use our monoclonal antibody cat#RDI-CALBINDabm directed against calbindin D-28k, which does not recognize calretinin.

dilutions: Immunohistochemistry: 1:10,000 - 1:20,000 with the avidin-biotin methods.

                Immunoblots: 1:100 - 1: 1,000

We recommend that the optimal dilutions be determined by titration experiments.

Storage: Reconstitute by shaking very long and sucking with a Pasteur-pipette until the pellet is dissolved. Make small portions (e.g.2-5 ul). For long storage, keep at -80'C (or at least -20'C). For continuous use keep at 4'C (with 0.01% Na-azide). Avoid repeated freezing and thawing.

For Research Use Only


Rabbit antibody to Calbindin D-9k.

CAT#: RDI-RTCALD9Kabr    $470.00/vial

LOT#:

Form: Lyophilized antiserum

Quantity: 200 ul

Reconstitution: with 200 ul bidistilled water.

Description: This antibody was produced against rat recombinant Calbindin D-9k. It can be utilized in immunohistochemistry and in immunoblots.

Dilutions: Immunohistochemistry: 1:1,000 - 1: 10,000 with the avidin-biotin method.

                  Immunoblots: 1: 1,000.

We recommend that the optimal dilutions be determined by titration experiments.

Storage: For long storage, keep at -80'C (or at least -20'C). For continuous use keep at 4'C (with 0.01% Na-azide). Avoid repeated freezing and thawing.

For Research Use Only


Calbindin-D9k (porcine intestine)

cat#RDI-CALBD9K-AG   $500.00/200ug  $438.00/vial 3+

White lyophilized solid. Intestinal vitamin D-dependent calcium-binding protein. Functions as a cytosolic buffer for Ca2+ ions in duodenal enterocytes and facilitates transepithelial active transport of Ca2+ ions. Also present in porcine uterus and placenta. Binds to two equivalents of Ca2+. Purity: >98% by HPLC. Sold on the basis of peptide content. Molecular Weight: 8840.0 Solubility: H2O Storage: Freezer (-20°C). This product is stable for 2 years as supplied.

Ref.: Schroder, B., et al. 1996. J. Physiol. 492, 715; Hitchman, A.J., and Harrison, J.E. 1972. Can. J. Biochem. 50, 758.


Recombinant rat calbindin D-9K

cat#RDI-RTCALBD9k-AG $312.00/10ug   $1000.00/60ug


Recombinant rat calbindin D-28K

cat#RDI-RTCALBD28K-AG $281.00/10ug $1000.00/100ug


RDI Divison of Fitzgerald Industries Intl

34 Junction Square Drive

Concord MA 01742-3049

USA

phone (978) 371-6446 or (800) 370-2222

fax     (978) 371-2266

EMAIL:antibodies@fitzgerald-fii.com

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