rev: November 11, 2003
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Anti-MOUSE
CD CLUSTERED
ANTIBODIES
RDI Division of Fitzgerald Industries Intl offers a wide line of antibodies. Since no one antibody works best for all applications (flow cytometry, neutralization, blotting, histochemistry, ELISA, etc), we offer many different types of antibodies to help solve this problem. Please inquire for other applications or types of antibodies not listed below. All products are for in vitro research use only-not for use in or on humans or animals-not for use in diagnostics. Price/availability/specifications subject to change without notice.
Anti-MOUSE CD86 (B7-2)
Antibodies
DATA SHEET: Rat anti mouse CD86 (B7-2), monoclonal
Catalog#: RDI-MCD86-GL1
Price: $500.00/1pkg
Package Size: 0.5mg in 0.5ml
Supplied: purified ab in buffer with 0.09%NaN3
Clone: GL1
Ig Isotype: rat IgG2ak
Purification: Purified from ascites
Reactivity: Clone GL1 reacts with the B7-2 co stimulatory molecule expressed
on antigen presenting cells:dendritic cells and activated B cells within
24 hours of stimuklation by LPS, anti-CD40, anti-surface IgG or specific
antigen. Clone GL1 blocks MLR and stimulation of T cells by natural
antigen-presenting cells. CD80 is an alternative ligand for CD28 and CTLA-
4.
USE: -flow cytometry(<1ug/1M cells) (positive control spleen cells)
-ligand blocking (no azide formulation)
-histochemistry:acetone fixed frozen sections
-immunoprecipitation
Note: in certain applications, the use of an Fc blocking reagent may be desirable to reduce non specific binding of GL1 antibody to cells bearing the FCgamma receptor (see ms CD16/32 ab).
ref: -Science 262:905-907
-Immunol Today 15:321-331
-Science 262:907-909
-Science 262:909-911
Storage: Store at 4 Deg C. Aliquot. Avoid frequent freeze thaw cycles.
Precautions: For In vitro research Use Only. Not for use in or on humans or animals or for diagnostics. Sodium azide may form explosive compounds in presence of heavy metals or under acidic conditions. Flush drains with copious amounts of water to prevent buildup of explosive compounds.
also available;
no azide formulation cat#RDI-MCD86-XP $625.00/0.5mg
FITC labeled cat#RDI-MCD86-GL1FT $594.00/0.5mg (Discontinued)
Biotin labeled cat#RDI-MCD86-GL1BT $562.00/0.5mg
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RDI Division of Fitzgerald Industries Intl
34 Junction Square Drive
Concord MA 01742-3049
USA
phone (978) 371-6446 or (800) 370-2222
fax (978) 371-2266
EMAIL:antibodies@fitzgerald-fii.com