rev:  June  21, 2005

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Anti-MOUSE
CD CLUSTERED ANTIBODIES  

RDI Division of Fitzgerald Industries Intl  offers a wide line of  antibodies. Since no one antibody works best for all applications (flow cytometry, neutralization, blotting, histochemistry, ELISA, etc), we offer many different types of antibodies to help solve this problem. Please inquire for other applications or types of antibodies not listed below. All products are for in vitro research use only-not for use in or on humans or animals-not for use in diagnostics. Price/availability/specifications subject to change without notice.

Anti-MOUSE  CD16/CD32  Antibodies

DATA SHEET:    Rat anti-mouse (CD32/16), monoclonal FC Gamma II/III  Receptor

Catalog#: RDI-MCD3216   Price: $500.00/1pkg 0.5mg    $219.00/0.1mg

Package Size: 0.5mg in 0.5ml

Supplied: purified ab in buffer with sodium azide preservative

Clone: 2.4G2

Ig Isotype: rat IgG2b

Purification: Purified from ascites

Reactivity: Reacts with mouse FC gamma II and FC gamma III receptors. This clone blocks non-antigen specific binding of antibodies to the mouse FCGII/III receptors. The FCGII/III receptors are expressed on murine natural killer cells, monocytes, macrophages, granulocytes, B lymphocytes and most fetal thymocytes prior to expression of CD4, CD8 and TCR.

USE: -flow cytometry (approx 1ug/million cells)

        -in vitro blocking of Fc mediated binding of antibodies to FCG receptor bearing cells. <1ug/million cells

       -reported for histochemistry on acetone fixed frozen sections using approx 10ug/ml and amplified detection

       -immunoprecipitation

       -activation of leukocytes by receptor cross linking

ref: -Unkeless, J.C. et al, J. Exp Med. 150:580

      -Titus, J.A. et al, J. Immunol. 133:556-561

      -Rodewald, H. et al, Cell 69:139-150.

      -J. Immunol 149:2155-2162

      -Infec. Imunol 61:4925-4928

Storage: Store at 4 Deg C. Aliquot. Avoid   frequent freeze thaw cycles.

Precautions: For In vitro research Use Only. Not for use in or on humans or animals or for diagnostics. Sodium azide may form explosive compounds in presence of heavy metals or under acidic conditions. Flush drains with copious amounts of water to prevent buildup of explosive compounds.

Also available FITC labeled,        cat#RDI-mCD3216-FT $562.00/0.5mg

                         Biotin conjugated  cat#RDi-MCD3216-BT $562.00/0.5mg

                        PE conjugated        cat#RDI-MCD3216-PE  $625.00/200u

   for cell culture work (unlabeled no azide)  cat#RDI-MCD1632-1XP $625.00/0.5mg

Precautions: For In vitro research Use Only. Not for use in or on humans or animals or for diagnostics. Sodium azide may form explosive compounds in presence of heavy metals or under acidic conditions. Flush drains with copious amounts of water to prevent buildup of explosive compounds.

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RDI Division of Fitzgerald Industries Intl

34 Junction Square Drive

Concord MA 01742-3049

phone (978) 371-6446

fax      (978) 371-2266

email: antibodies@fitzgerald-fii.com

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