rev:  September 2, 2003

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  ANTIBODIES  

                                                       (anti-Human and others as indicated)
RDI Divison of Fitzgerald Industries Intl  offers a wide line of  antibodies. Since no one antibody works best for all applications (neutralization, blotting, ELISA, etc), we offer many different types of antibodies to help solve this problem. Please inquire for other applications or types of antibodies not listed below.


Anti-FATTY ACID BINDING PROTEIN (see also pure antigens available below-)


-4 clones and pure anitgen available


Monoclonal mouse anti-human fatty acid binding protein (CARDIAC)

CAT#: RDI-FABP-5B5 ,  #RDI-FABP-9F3,  #RDI-FABP-6B6 ,   #RDI-FABP-10E1

        $438.00/mg      $375.00/mg 2-9mg     BULK QUOTES ON REQUEST


Clone#: 5B5, 9F3, 6B6, 10E1

These clones have been derived from hybridization of Sp 2/0 myeloma cells with spleen cells of Balb/c mice immunized with human fatty acid binding protein (FABP).


FABP is a newly introduced plasma marker of acute myocardial infarction (AMI). The plasma kinetics of FABP (15kD) closely resemble those of myoglobin in that elevated plasma concentrations are found within 3 hour after AMI and return to normal generally within 12 to 24 hours. This makes FABP useful biochemical markers for the early assessment of exclusion of AMI.The myocardial tissue content of FABP (0.5mg/g) is 5-fold lower than that of myoglobin (2.5mg/g), but the reference plasma concentration of FABP (ca. 2ug/L) is about 15-fold lower than that of myoglobin (ca. 32mg/L), together suggesting a superior performance of FABP for early detection of AMI. FABP also appears a useful plasma marker for the estimation of myocardial infarct size.

Specificity: Clone 5B5, 9F3, 6B6, 10E1 - human fatty acid binding protein. Specific antibody titer is 10-6.

Mouse isotype: Clone 6B6 - IgG2b, clones 5B5,  9F3,   6B6,   10E1 - IgG1

Application: Human fatty acid binding protein 1 immunoassay and immunoaffinity purification. All antibodies are working in ELISA.We recommend to use following pairs of antibodies for sandwich ELISA (coating antibody - antibody for conjugate): 10E1-9F3,   10E1-5B5,    9F3-10E1,    5B5-9F3.

The best combination for sandwich immunoassay is: 9F3 MAb used as capture antibody and 10E11 as a detection MAb

-for western blot: clone 6B6

Purification: Purified by chromatography on protein A Sepharose.Purity is tested by electrophoresis.

Presentation: Each vial contains 1 milligram antibodies with concentration (see batch sheet) in PBS, pH 7.4, containing 0.1% of sodium azide as preservative.

Store at +4C.

Material safety note: This product is sold as an antibody preparation for research purpose only. Standard Laboratory Practices should be followed when handling this material. Contains sodium azide (0.1%) as preservative. Although the amount of sodium azide is very small appropriate care must be taken when handling this product.


see also antigen to FABP and our Troponin and myoglobin products
see also anti-human liver fatty acid   binding protein antibody

For IN VITRO RESEARCH USE ONLY-NOT FOR USE IN OR ON HUMANS OR ANIMALS-

Not responsible for any patent infringements with use or derivation of this or any product.


Human Fatty Acid Binding Protein Antigen (CARDIAC)

cat# RDI-FABP-AG     $500.00/100ug     $1000.00/500ug     $1625.00/1mg

                             

FABP is a newly introduced plasma marker of acute myocardial infarction (AMI). The plasma kinetics of FABP (15kD) closely resemble those of myoglobin in that elevated plasma concentraions are found within 3 hour after AMI and return to normal generally within 12 to 24 hours. This makes FABP useful biochemical markers for the early assessment of exclusion of AMI. The myocardial tissue content of FABP (0.5mg/g) is 5-fold lower than that of myoglobin (2.5mg/g), but the  reference plasma concentration of FABP (ca. 2ug/L) is about 15-fold lower than that of myoglobin (ca. 32mg/L), together suggesting a superior performance of FABP for early detection of AMI. FABP also appears a useful plasma marker for the estimation of myocardial infarct size.

Package: lyophilized powder. Recommend reconstituting with distilled water.

Purity: >98% as determined by a 10-20% gradient SDS-PAGE. The immunological reactivity was confirmed with a monoclonal   antibody that is specific for the fatty acid binding   protein.

USE: Suitable as a standard in immunoassay for early detection of acute myocardial infarction, as an immunogen for antisera production and as a racer for iodination.

Storage: Store at -20 DEG C. Avoid frequent freeze thaw cycles.

Warning: Made with human heart tissue. Blood samples from tissue donors were tested and found negative for HbSAg, HIV-1 and   HIV-2 antibodies and HCV. Treat as if capable of   transmitting infection Handle at Biosafety Level II   conditions.

For IN Vitro Research Use Only-Not For Use IN Or on Humans Or Animals.


DATA SHEET:Human FATTY ACID BINDING PROTEIN  (CARDIAC) FREE SERUM

cat#RDI-FABPSERA     $938.00/100ml      $1188.00/500ml      $1750.00/Liter

Source:   Pooled normal human serum. Blood samples from donors were tested and found to be negative for HBsAg, HIV-1 and HIV- 2 antibodies, HCV.

Preparation: Affinity chromatography of pooled normal human serum Gel for affinity column was prepared by coupling anti-FABP monoclonal antibodies (several monoclonal antibodies specific for different epitopes of FABP molecule) with BrCN-activated Sepharose CL-4B.

Analysis: Does not contain detectable amounts of human FABP.


Applications: FABP free serum can be used for preparation of   human FABP standards and calibrators.


Storage: -20'C

Precautions: For In vitro research Use Only. Not for use in or on humans or animals or for diagnostics . All material should be handled at Biosafety Level II conditions and treated as if capable of transmitting disease.It is the responsibility of the user to comply with all local/state and Federal rules in the use of this product. We are not responsible for any patent infringements that might result with the use of or derivation of this product.


RDI Divison of Fitzgerald Industries Intl

34 Junction Square Drive

Concord MA 01742-3049

USA

phone (978) 371-6446 or (800) 370-2222

fax     (978) 371-2266

EMAIL:antibodies@fitzgerald-fii.com

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