rev:     November 3, 2003

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  ANTIBODIES  

(anti-Human and others as indicated)

RDI Divison of Fitzgerald Industries Intl  offers a wide line of  antibodies. Since no one antibody works best for all applications (neutralization, blotting, ELISA, etc), we offer many different types of antibodies to help solve this problem. Please inquire for other applications or types of antibodies not listed below.


Anti-Cadherin Antibodies  (see below polyclonals and monoclonals)

E-Caderin,  K-Cadherin, L1-cadherin, N-Cadherin, M-Cadherin, OB-cadherin, P-Cadherin,  R-Cadherin, T-cadherin

 Cadherin (VE) anti-human, or anti-mouse VE-Cadherin CD144)



click here for 2additional E-cadherin monoclonals, cat#RDI-PRO10704 & cat#RDI-PRO10028  

Mouse anti-E Cadherin Monoclonal ab

cat# RDI-ECADHERabm        $438.00/vial 50ug

       RDI-ECADHERabm-1      $750.00/vial 150ug

       RDI-ECADHERabm-FT    $500.00/50ug  FITC conj


Background: E-Cadherin is a 120Kda transmembrane glyoprotein that is localized in the adherens junctions of epithelial cells and interacts with the cytoskeleton through the associated cytoplasmic catenin proteins. In addition to being a calcium-dependent adhesion molecule, E-cadherin is also a critical regulator of epithelial junction formation. E-Cadherin association with catenins appears to be necessary for cell-cell adhesion. These complexes associate with cortical actin bundles at both types of adherens junctions-the zonula adherens and the lateral adhesion plaques.

isotype: mIgG2a

package: 50ug (200ul) or 150ug (600ul) (at 0.25mg/ml in 50% glycerol, PBS + 1.5mM NaN3 & 1mg/ml BSA)

antigen: a 16.5 kDa C-terminal protein fragment corresponding to amino acids 735-883 of human E- cadherin


USe: Suitable for western blot on human under using approx 0.1 to 0.05ug/ml ab on total cell lysate of A431 cells. Major band =MW 120kDa.  Immunoreactivity also shown with dog, rat and mouse. Immunofluorescence .


Storage: Store at -20 DEG C upon receipt. . Recommend aliquoting. Avoid frequent warming cycles.


Main REF: -Tamm. I. et al (1994). Proc. Natl. Acad. Sci USA 91:4338

                 -Behrens, J. et al (1993) J. Cell Biol. 120:757


Precautions: For In vitro research Use Only. Not for use in or on humans or animals or for diagnostics. It is the responsibility of the user to comply with all local/state and Federal rules in the use of this product. We are not responsible for any patent infringements that might result with the use of or derivation of this product.


Also available: mono's and poly's against P-Cadherin, R-Cadherin, and the catenin's


Mouse anti-N Cadherin Monoclonal ab

cat# RDI-NCADHERabm        $438.00/vial 50ug

       RDI-NCADHERabm-1     $750.00/vial 150ug

Background: Cadherins are a family of CA2+ dependent intercellular adhesion molecules that play a central role in controlling morphogenetic movements during development. Their function is regulated by association with the actin cytoskeleton by a complex of cytoplasmic proteins called the catenin (alpha, beta and gamma). Members of the cadherin family include P-cadherin, E-cadherin (uvomorulin), N-cadherin (neural cadherin), R-Cadherin, cadherin 5, L-CAM and EP-Cadherin. N-Cadherin mRNA is found at elevated levels in brain and heart and at much lower level in liver. Mechanisms such as mRNA expression, cytokine modulation and protease mediated turnover modulate N-cadherin protein levels during development. In addition, N-cadherin function is indirectly regulated by endogeneous kinases and phosphatases. Tyrosine phosphorylation of Beta-Catenin complexed with N-Cadherin results in dissociation of N-Cadherin from actin. However, N-Cadherin also interacts with a PTP1-B-like phosphatase that dephosphorylates B-catenin and promotes N-Cadherin/actin association. This, N-Cadherin is an integral adhesion molecule whose function is regulated by protein-protein interactions and phosphorylation/dephosphorylation events.

isotype: mIgG1

package: 50ug (200ul) or 150ug (600ul) (at 0.25mg/ml in 50% glycerol, PBS + 1.5mM NaN3 & 1mg/ml BSA)

antigen: a C-terminal region pepide corresponding to amino acids 802-   819 of mouse N-cadherin K P V G I R R L D E R P I H A E P Q     (control peptide available cat#RDI-NCADHERM-CP $156.00)

Use: Suitable for western blot on human, rat, mouse, and chick under using approx 0.1 to 0.5ug/ml ab on total cell of Rat brain (2-3 Hour RT or overnight at 4 DEg C with chemiluminescence detection). Major band =MW 130kDa.


Storage: Store at -20 DEG C upon receipt. . Recommend aliquoting. Avoid frequent warming cycles.

Main REF: -Packer, A.I. et al. 1997 Int J Dev Biol 41:459-468

                   -Lee, M.M. et al. 1997 Dev Genet 20:224-234

                  -Balsamo J., et al. 1996 J Cell. Biol. 134:801-813

Precautions: For In vitro research Use Only. Not for use in or on humans or animals or for diagnostics. It is the responsibility of the user to comply with all local/state and Federal rules in the use of this product. We are not responsible for any patent infringements that might result with the use of or derivation of this product.


Mouse anti-P Cadherin Monoclonal ab

cat# RDI-PCADHERabm      $438.00/vial 50ug

        RDI-PCADHERabm-1  $750.00/vial 150ug

Background: P-cadherin is an 882 amino acid polypeptide with a putative signal peptide, a putative precursor region, an extracellular domain containing internal repeats, and a highly hydrophobic transmembrane region. Amino acid sequences among P-, E- and N-cadherin are most conserved in the cytoplasmic domain followed by conservation of the N-terminal region of the extracellular domain. The cytoplasmic domain provides a link to the cytoskeleton through the associated catenin proteins. The mouse P-cadherin genes have been shown to be tightly linked on chromosome 8. The human E-cadherin gene has been localized to chromosome 16p11-16qter.

isotype: mIgG1


package: 50ug (200ul) or 150ug (600ul) (at 0.25mg/ml in 50% glycerol, PBS + 1.5mM NaN3 & 1mg/ml BSA)

antigen: a 21.1 protein fragment corresponding to amino acids 72-259 of human P- cadherin

Suitable for western blot on human and mouse using approx 1ug/ml ab (2-3 hours RT or overnight 4 DEG C with chemiluminescence detection) on total cell lysate of human A431 cells. Major band =MW 120kDa. Immunoreactivity shown with human ,mouse and rat Immunofluorescence, immunohistochemistry and immunoprecipitation (denaturing conditions).

Storage: Store at -20 DEG C upon receipt. . Recommend aliquoting. Avoid frequent warming cycles.


Main REF: -Hatta, M. et al (1991) Nucleic Acids Res. 19:4437

                  -Takeichi, M. (1988) Development 102:639

Precautions: For In vitro research Use Only. Not for use in or on humans or animals or for diagnostics. It is the responsibility of the user to comply with all local/state and Federal rules in the use of this product. We are not responsible for any patent infringements that might result with the use of or derivation of this product.


Mouse anti-RAT R Cadherin Monoclonal ab

cat# RDI-RCADHERabm       $438.00/vial 50ug

        RDI-RCADHERabm-1   $750.00/vial 150ug


Background: R-cadherin was first isolated from chicken retina, but was subsequently shown to be present and highly conserved in a wide variety of tissues from several different animals. A 913 amino acid polypeptide, R-cadherin is homologous to cadherin-4,and is most closely related to other family member, N-cadherin.In experiments using chimeric L cells expressing chicken R-cadherin, and mouse R-, N-, E- and P-cadherins, those cellsexpressing mouse or chicken R-cadherin were able to aggregate with each other as well as with cells expressing N-cadherin.

isotype: mIgG


package: 50ug (200ul) or 150ug (600ul) (at 0.25mg/ml in 50% glycerol, PBS + 1.5mM NaN3 & 1mg/ml BSA)

antigen: a 20 kDa protein fragment corresponding to amino acids 22-2-1 of mouse E-cadherin


Use: Suitable for western blot on rat brain using approx 0.1 to 0.05ug/ml . Major band =MW 120kDa

Storage: Store at -20 DEG C upon receipt. . Recommend aliquoting. Avoid frequent warming cycles.


Main REF: -Tanihara, H. et al (1994) Cell Adhes. Commun. 2:15

                 -Matsunami, H. et al, (1993) J. Cell Sci 106:401

                 -Hutton, J.C. et al (1993) Mol Endocrinol. 7:1151.

Precautions: For In vitro research Use Only. Not for use in or on humans or animals or for diagnostics. It is the responsibility of the user to comply with all local/state and Federal rules in the use of this product. We are not responsible for any patent infringements that might result with the use of or derivation of this product.


rev:April 23, 1997

Mouse anti- Cadherin 5 Monoclonal ab

cat# RDI-CADHER5abm        $438.00/vial 50ug

       RDI-CADHER5abm-1    $750.00/vial 150ug

Background: Cadherins are a family of transmembrane glycoproteins involved in the CA 2+ dependent cell-cell adhesion that occurs in many types of tissues. These proteins are similar in their domain structure (45-74% amino acid conservation), Ca 2+ and protease-sensitivity and in molecular weight. Cadherin-5 is one of a number of cadherins (Cadherin-4 through -11) whose cDNAs were initially isolated from rat brain and retina. These cadherins have a cytoplasmic domain which is highly conserved relative to the previously identified cadherins, further substantiating the idea that this domain is essential for cell adhesion activity. It is believed that this function is mediated by cadherin interaction with cytoskeletal proteins. However, Cadherin-5's cytoplasmic domain has the lowest degree of homology with the other cadherins. Cadherin-5 is expressed in brain and various other tissues, including umbilical cord vein endothelial cells. Recently a new type of adhering junction has been identified in certain vascular endothelial cells. These junctions are known as "complexus adherens" and are morphologically and compositionally distinct from desmosomes and zonula adheren junctions. The complexus adherens of these endothelial cells lack desmosomal cadherins as well as E-Cadherin. However, these cells are rich in Cadherin-5 which co-localizes with desmoplakin and gamma catenin (plakoglobin).


isotype: mIgG1


package: 50ug (200ul) or 150ug (600ul) (at 0.25mg/ml in 50% glycerol, PBS + 1.5mM NaN3 & 1mg/ml BSA)


antigen: N-terminal region protein fragment corresponding to amino acids 26-194 of human Cadherin 5


Use: Suitable for western blot on human under using approx 0.1 to 0.1ug/ml ab on total cell of human endothelial cells (positive control). Major band =MW 130kDa. Immunoreactivity also shown in Immunofluorescence


Storage: Store at -20 DEG C upon receipt. . Recommend aliquoting. Avoid frequent warming cycles.


General REF: -Suzuki, S. et al. 1991 Cell Reg 2:261

                      -Schmeiz, M. et al, 1993 Eur J. Cell Biol 61:274

                       -Leach, L. et al. 1993 J. Cel Sci 104:1073


Precautions: For In vitro research Use Only. Not for use in or on humans or animals or for diagnostics. It is the responsibility of the user to comply with all local/state and Federal rules in the use of this product. We are not responsible for any patent infringements that might result with the use of or derivation of this product.

copyright by owner

Also available: mono's and poly's against P-Cadherin, R-Cadherin,E-Cadherin, K-Cadherin, OB-Cadherin and the catenin's


Mouse anti-K Cadherin Monoclonal ab -discontinued Jan 99

RDI Divison of Fitzgerald Industries Intl

34 Junction Square Drive

Concord MA 01742-3049

USA

phone (800) 370-2222

      or (978) 371-6446 or (800) 370-2222

fax     (978) 371-2266

EMAIL:antibodies@fitzgerald-fii.com

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