rev:  June 20, 2003

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  ANTIBODIES  

(anti-Human and others as indicated)

RDI Divison of researchd Industries Intl  offers a wide line of  antibodies. Since no one antibody works best for all applications (neutralization, blotting, ELISA, etc), we offer many different types of antibodies to help solve this problem. Please inquire for other applications or types of antibodies not listed below.

PRICE: $375.00/mg  or    $312.00/mg    2 or more

Clone: 2E9

This clone has been derived from hybridization of Sp2/0 myeloma cells with spleen cells of a balb/c mice immunized with purified beta galactosidase of E. Coli.

Specificity: galactosidase of E.Coli

Reactivity: MoAb 2E9 reacts with beta galactosidase of E. Coli and Aspergillus oryzae. Cross reactivity with beta galactosidase from bovine liver less than 10%. This Mab does not influence enzyme activity. Specific titer in indirect elisa 1:1 million.

Mouse isotype: IgG1, kappa

Application: Detection of  beta galactosidase expressed by LacZ gene in cloned sequences.

Presentation:Each vial contains 1 ml of antibodies with concentration 1 mg/ml, in PBS, pH 7.4, containing 0.1% of sodium azide as preservative. Store at -20C. Avoid repeated freeze-thaw cycles.

Material safety note: This product is sold as an antibody preparation for research purpose only. Standard Laboratory Practices should be followed when handling this material. Contains sodium azide (0.1%) as preservative. Although the amount of sodium azide is very small appropriate care must be taken when handling this product.

FOR IN VITRO RESEARCH USE ONLY

DATA SHEET: Rabbit anti-Beta Galactosidase

Catalog#: RDI-BGALACTOabr     $312.00/vial   

Also available HRP conjugated: cat#RDI-BGALACT-HRP     $440.00/1020mg

                                 Biotin conj: cat#RDI-BGALACT-BT       $440.00/10mg

                                  FITC conj   cat#RDI-BGALACT-FT      $440.00/10mg

Size: 10 mg (lyophilized)

Antibody Concentration: approx 10mg/ml

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride,pH 7.2

Stablizer: None

Preservative: 0.01% (w/v) Sodium Azide

Antigen: B Galactosidase (from E. Coli)

Applications: suitable for immunoblotting (western or dot blot),ELISA, immunoprecipitation, conjugation and most immunological methods requiring high titer and specificity.

Activity: binds to the bacterial form of the enzyme and may be used to detect any b- galactosidase expression by the lacZ gene.Shows a band of 464 Kda (tetramer) under non-reducing conditions of SDS-Page and a 115Kda under reducing conditions (Laemmli 4-20 % gradient SDS-PAGE)

Recommended Dilutions: Not determined. Optimal titers for applications should be determined by the researcher.

Storage Conditions: Store vial at 4'C prior to restoration. Re-store with 5.0 ml of deionized water (or equivalent). Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4'C as an undiluted liquid. Dilute only prior to immediate use. For extended storage aliquot contents and freeze at -20'C or below. Avoid cycles of freezing and thawing. Expiration date is one (1)year from date of reconstituion.

For In vitro research Use Only. Not for use in or on humans or animals or for diagnostics.

San Jose, 95123 CA Snell ave 658

USA

or (800) 370-2222

EMAIL:margaret@cellular-research.com

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