rev: March 29, 2004
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ANTIBODIES
(anti-Human and others as indicated)
RDI Divison of Fitzgerald Industries Intl offers a wide line of antibodies. Since no one antibody works best for all applications (neutralization, blotting, ELISA, etc), we offer many different types of antibodies to help solve this problem. Please inquire for other applications or types of antibodies not listed below.
-see also pure antigen
(Not reactive
with bovine, pig, sheep, dog, cat, mouse or chicken)
Uses: histochemistry (frozen and paraffin sections, for paraffin sections,
digest the sections with 1mg/ml trypsin in PBS or 5 minutes at RT).) approx
1:50-1:100 with 30 minutes RT incubation
Positive Control tonsil or colon,lung carcinoma
Localization: Cytoplasmic
Background: recognizes a protein of 51Kda, identified as alpha-1- antitrypisn
which exists in a number of genetic variants. The MM variant is most common.
Alpha-1AT is synthesized in the liver and it acts as an inhibitor of proteases
such as trypsin, elastase, chymotrypsin, colleganase, leucocyte proteases,
plasmin, and thrombin, which may be released during inflammatory reactions
int he lung. In the absence of alpha-1-AT, these enzymes are not inhibited
and they may digest pulmonary parenchyma.Alpha-1AT deficiency is associated
with chronic obstructive lung disease (emphysema) and less frequently with
hepatic cirrhosis in infants and respiratory distress of the newborn. Increase
in Alpha-1-AT occurs as an acute phase response to tissue necrosis and
inflammation. Serum level of Alpha-1AT is elevated in rheumatoid arthritis,
bacterial infections, vasculitis, and carcinomatosis. In colorectal and lung
carcinomas, alpha-1- AT has also related to their invasive and metastatic
capacity.
Storage: 2-8 deg c
For Research Use Only
Catalog#: RDI-A1ATRYPabG $438.00/vial
Also available HRP conjugated: cat#RDI-A1ATRYP-HRP $469.00/20mg vial
Biotin conjugated #RDI-A1ATRYP-BT $469.00/10mg vial
Size: 50 mg (lyophilized)
Antibody Concentration: 10.0 mg/ml (by UV absorbance at 280 nm)
Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride,pH 7.2
Stablizer: None
Preservative: 0.01% (w/v) Sodium Azide
Antigen: alpha-1-anti-trypsin (human plasma)
Applications: suitable for immunoblotting (western or dot blot),ELISA and
immunoprecipitation,
Background: reacts with alpha-1-acid antitrypsin from human plasma showing a 45kDa band under reducing conditions of SDS-Page.
Recommended Dilutions: Not determined. Optimal titers for applications should
be determined by the researcher.
Storage Conditions: Store vial at 4'C prior to restoration. Re-store with
5.0 ml of deionized water (or equivalent). Centrifuge product if not completely
clear after standing at room temperature. This product is stable for several
weeks at 4'C as an undiluted liquid. Dilute only prior to immediate use.
For extended storage aliquot contents and freeze at -20'C or below. Avoid
cycles of freezing and thawing. Expiration date is one (1)year from date
of reconstitution)
Purity and Specificity: This product is an IgG fraction antibody purified
from monospecific antiserum by a multi-step process which includes delipidation,
salt fractionation and ion exchange chromatography followed by extensive
dialysis against the buffer stated above. Assay by imunoelectrophoresis resulted
in a single precipitin arc against anti- Goat Serum as well as purified and
partially purified alpha-1-AT (human plasma)
Precautions: For In vitro research Use Only. Not for use in or on humans
or animals or for diagnostics. It is the responsibility of the user
to comply with all local/state and Federal rules in the use of this product.
We are not responsible for any patent infringements that might result with
the use of or derivation of this product.
34 Junction Square Drive
Concord MA 01742-3049
USA
phone (800) 370-2222
or (978) 371-6446 or (800) 370-2222
fax (978) 371-2266
EMAIL:antibodies@fitzgerald-fii.com
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