rev:  September10, 2003

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Anti- Virus  ANTIBODIES  

(anti-Human and others as indicated)

RDI Divison of researchd Industries Intl  offers a wide line of  antibodies. Since no one antibody works best for all applications (neutralization, blotting, ELISA, etc), we offer many different types of antibodies to help solve this problem. Please inquire for other applications or types of antibodies not listed below.


Mab to  HIV-1 p24  (see 4 clones & 1 polyclonal below)

see also pure viral protein


Monoclonal mouse anti-HIV p24

CAT#: RDI-TRK3H24-ND1

PRICE: $375.00/mg or $344.00/mg 2-9 or more    $200.00/mg 50+ Bulk on quotation

Clone: ND1   This clone has been derived from hybridization of Sp2/0 myeloma cells with spleen cells of a Balb/c mice immunised with p24 C terminal peptide.

Specificity: HIV-1 p24 by immunoblot/Elisa

Mouse isotype: IgG1

Application: Detection of P24 in Elisa and western blot

Purification: Purified by DEAEcchromatography Purity is tested by electrophoresis.

Presentation: Each vial contains 1 mg of antibodies with con- centration mg/ml in 0.ml,in PBS, pH 7.4, containing 0.1% of sodium azide as preservative.

Material safety note: This product is sold as an antibody preparation for research purpose only. Standarad Laboratory Practices should be followed when handling this material. Contains sodiium azide (.01%) as pre- servative. Although the amount of sodium azide is very small appropriate care must be taken when handling this product.

FOR IN VITRO RESEARCH USE ONLY

NOTE:

Recommend centrifuging the unopened vial at 500-1000 RPM for 2 minutes to concentrate antibody in bottom of vial. If desired, you can add ul PBS containing 0.1% NaN3 to bring volume to 1mg in 1ml


DATA SHEET: Mouse Monoclonal anti-HIV-I GP24

Catalog#: RDI-HIVP24abm-26  - Discontinued

Price: $500.00 plus shipping    $438.00/vial 2 or more


Package Size: 100ug/vial

Supplied: lyophilized: Reconstitute with 0.5ml sterile DI H20 to yield a 200ug/ml stock in PBS pH 7.2. Let set at least 30 minutes at 4-8 DEG C , Mix well. If desired, centrifuge for 1-2 minutes at 1000 rpm to concentrate antibody in vial.

Ig Isotype: IgG2b

USE: Binds to HIV-1 p24 in Western blot and Elisa. approx epitope is on peptide 25 (aa 154-172)

Minimum dilution: 1:200 (for blotting, overnight 4 DEG C with chemiluminescence detection)

Storage: Store at 4-8 DEG C. After reconstitution, recommend aliquoting and store in working aliquots at -20 DEg C or below. Avoid frequent freeze thaw cycles.

Precautions:For In vitro research Use Only. Not for use in or on humans oranimals or for diagnostics. It is the responsibility of the user to comply with all local/state and Federal rules in the use of this product. We are not responsible for any patent infringements that might result with the use of or derivation of this product.

see also antibodies to: gp120, gp41, tat, rev, nef, RT, integrase and more.. available purified, FITC, Biotin and Hrp conj


Goat anti-HIV P24 (aff pure)  Polyclonal

cat# RDI-HIVP24abGX

Price: $188.00/1mg $125.00/mg 5+  Bulk on quotation

Presentation: IgG fraction in PBS buffer, pH 7.2 with 0.1% NaN3.

Immunogen: Highly pure HIV-1 p24

Host: goat

Titer: approx 1:100,000 tests/ml by EIA ; 5.0 mg/ml

Cross Reactivity: Specific for p24 ; No reactivty to human T or B cells.

Storage: 4°C or -20°C for long term storage

Remarks: Tested by EIA and Western blot

For Research or Futher Manufacturing Use Only


anti-HIV-1 p24 antibody (monoconal)


CAT#: RDI-HIVP24abm   $375.00/100ug   $938.00/1mg  Bulk on request


Description: HIV-1 p24 IgG (monoclonal). Produced against a recombinant protein.


Specificity: HIV-1 p24 antigen.


Origin: Mouse


Clone: 1A1


Purity: Affinity purified

Buffer: 1X PBS pH 7.2;0.01% sodium azide.

Concentration:-1 mg/ml;bulk amount available upon request.

Recommended Dilution: For ELISA: 1:1000. For Western Blot: 1:1000.

Storage: Short term: 4'C. Long term: -20'C. Avoid frequent freeze thaw cycles

For In vitro research Use Only. Not for use in or on humans or animals or for diagnostics.It is the responsibility of the user to comply with all local/state and Federal rules in the use of this product. We are not responsible for any patent infringements that might result with the use or derivation of this product.

Note:  Can be custom

FITC conjugated  cat#RDI-HIVP24abm-FTX    $1250.00/1 milligram   $1125.00/mg 2 or more

or biotin labeled  cat#RDI-HIVP24abm-BTX    $1250.00/1 milligram   $1125.00/mg 2 or more


Mouse anti-HIVp24 capsid antigen (Paraffin Sections)

cat# RDI-HIVP24abm-K1  $500.00/vial

Specificity: Human immunodeficiency virus (HIV) type 1 capsid protein p24 and its precursor,p55. No reaction is observed with HIV type 2 or simian immunodeficiency virus

Clone: Kal-1

Ig Class: IgG1k

Antigen: purified human immunodeficiency virus type 1 disrupted with 0.5% NP40

Hybridoma partner: Mouse myeloma (p3-X63-Ag8.563).

Preparation: Lyophilised tissue culture supernatant containing 15mM sodium azide. Reconstitute with 1ml of sterile distilled water.

USE: Effective on paraffin wax embedded tissue using 1mM EDTA pH 8.0 unmasking solution combined with the high temperature antigen unmasking technique -not tested for frozen sections (see other clones)

Protocol: Immunohistochemistry:Typical working dilution 1:10 - 1:25. High temperature antigen unmasking technique using 1mM EDTA (pH 8.0). 60 Minutes incubation at 25'C. Standard ABC technique.

Positive controls: HIV-positive lymph node

Staining pattern: granular staining in close proximity to the membrane of infected cells -reacts with lymphocytes, monocytes, macrophages, langerhans cells of the skin, follicular dendritic cells and brain cells of microglia lineage infected with HIV type 1.

Storage and stability: Store unopened lyophilized antibody at 4'C. Under these conditions, there is no significant loss in product performance up to the expiry date indicated on the vial label. The reconstituted antibody is stable for at least two months when stored at 4'C. For long term storage, aliquot in non frost free freezer, Avoid repeated freeze thaw cycles. Prepare fresh working dilutions daily.

Refs: -Path Res Pract 188:91-96(1992)

         -American Journal f Pathology, 137(2):247-251 (1990)

Stricly for in vitro research use only-not for use in diagnostics

Sample High Temperature Unmasking Technqiue for Paraffin Sections:

Buffer=1mM EDTA (ph 8.0) or 0.01M sodium Citrate Buffer (pH 6.0)

1) Cut and mount sections on slides coated with "apes"

2) Deparrafinize sections and rehydrate to distilled water

3) Bring 1600ml 1mM EDTA* (pH 8.0) to a boil in a Prestige/Presto stainless steel pressure cooker, using a hot plate. Cover but do not lock lid.   *or 0.01M sodium citrate buffer (pH 6.0)-not for CD4 ab

4) Position slides into metal staining racks and lower into pressure cooker ensuring slides are well immersed in buffer Lock the lid. The small valve will rise.

5) When the pressure indicator valve (the large one) has risen after about 4minutes, Incubate sections for 1 minute.

6) After timer rings, Remove pressure cooker from heat source and run under cold water with lid on. When the small valve sinks open lid and remove slides and place immediately into distilled water. DO NOT OPEN LID UNTIL THE SMALL VALVE Sinks or Vent Cover Lock Drops (see manual with your particular unit).

7) Wash sections PBS buffer (10mM phosphate, 0.15M NaCl pH 7.5) for 2 X 5 minutes (or 100mM TRIS, 0.15M NaCl, pH 7.5)

8) Place sections in 1.5% hydrogen peroxide/methanol for 10 minutes (or use other appropriate peroxidase blocking procedure).

9) Wash sections in distilled water for 2 X 5 minutes, then wash sections in PBS buffer for 2 X 5 minutes.

10) Place sections in normal serum for 20 minutes.

|11) Cover sections with primary antibody for at least 60 minutes at room temp. (Optimum dilution, incubation time and temperature must be determined for each antibody and in each lab).

12) Wash in PBS buffer for 2 X 5 minutes

13) Incubate sections in secondary antibody for 30 minutes

14) Wash in PBS buffer for 2 X 5 minutes

15) Incubate slides in ABC complex for 30 minutes

16) Wash in PBS buffer for 2 X 5 minutes

17) Incubate slides in DAB or other suitable peroxidase substrate

18) Wash in distilled water for 2 X 5 minutes

19) Counterstain with haematoxylin (if required), dehydrate, coverslip, and mount

Material:

APES (3-aminopropyltriethoxysilane) cat#A3648 Sigma

To avoid sections becoming detached, sections should be mounted on "APES"  covered slides, then dried at 37 DEG C overnight followed by drying at 56 DEG  C for 60 minutes.

1mM EDTA (ph 8.0): Add 0.37g of EDTA (Sigma cat#E-5134) to 1 liter of distilled  water. Adjust pH to 8.0 using 1.0M sodium hydroxide.

Tris: 100mmM Tris, 0.15M NaCl, pH 7.5.

PBS: 10mM Phosphate, 0.15M NaCl pH 7.5


RDI Divison of researchd Industries Intl

San Jose, 95123 CA Snell ave 658

USA

or 408-780-0908

EMAIL:margaret@cellular-research.com

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