rev: September 25, 2003
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CD CLUSTERED ANTIBODIES
(anti-Human and others as indicated)
NOTE: 10% Discount on purchases of 3 or more any combination of monoclonal CD antibodies. Please inquire for bulk or custom formulations (without preservative or carrier).
RDI Divison of researchd Industries Intl offers a wide line of antibodies. Since no one antibody works best for all applications (flow cytometry, neutralization, blotting, histochemistry, ELISA, etc), we offer many different types of antibodies to help solve this problem. Please inquire for other applications or types of antibodies not listed below. All products are for in vitro research use only-not for use in or on humans or animals-not for use in diagnostics. Price/availability/specifications subject to change without notice.
CATALOG# CLONE# Workshop Host Form Price
RDI-CD83-HB15A HB15E VI mIgG1 purified $594.00
Catalog#: RDI-CD83HB15A $594.00/vial
Package Size: 200ug in 0.2ml Phosphate buffered Saline & 0.09% sodium azide. (sodium azide is toxic, dilute azide compounds with copious amounts of water when disposing in drain to avoid accumulation of explosive deposits-avoid contact with acids.)
Species: mouse IgG1
CLONE: HB15e (HB15A17.11)**
Antigen: Cos cells transfected with HB15A cDNA
Fusion Partner: spleen cells from immunized BALB/c mice were fused with cells of the mouse NS1 myeloma cell line
Activity: Clone HB15A antibody recognizes the human CD83 cell surface antigen, a 40-45kD glycoprotein expressed by peripheral blood dendritic cells. Peripheral lymphocytes can be induced to express very low levels of CD83 after culture in agents such as Con A or PHA. -In immunohistology, CD83 is shown to be expressed strongly by interfollicular interdigitating reticulum cells and more weakly by cells within germinal centers. CD83 is also expressed by Langheran's cells in the skin.
Application: -Indirect immunofluorescence cell surface staining (Flow) , 10ul of 1:50-1:100/1million cells
-histochemistry (frozen sections 1:500-1:1000 -paraffin sections 1:50-1:100 (positive control human tonsil) Requires protein digestion pre- treatement of paraffin sections ie, trypsin or pronase prior to staining. -immunoprecipitation
Species: also reported to cross react with Cynomolgus monkey
ref: -J. Immuol. 149(2):735-742 (1992)
-J. Immunol 154(18):3821-3835
-Leucotpe Typing VI, Garland Publishing 1997
Storage: Store at 4 DEG C (up to one month) or -20 DEG C longer. Recommend aliquoting. Avoid frequent freeze thaw ccyles.
Precautions: For In vitro research Use Only. Not for use in or on humans or animals or for diagnostics.
** Note: The clone owner has indicated that this clone has also been known as clone HB15e some for uniformity in literature, the clone will be only reported as HB15e.
mouse anti-human CD83 clone HB15e also available:
cat#RDI-CD83-HB15E $469.00/100ug purified
cat#RDI-CD83-HBEFT $531.00/100 tests FITC conjugated
cat#RDI-CD83-HBEPE $594.00/100T PE conjugated
ref: Blood 90:3245 (1997)
J. Immunol 154:3821 (1995)
Proc Natl Acad Sci USA 92:826 (1995)
San Jose, 95123 CA Snell ave 658
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