rev: October 3, 2001
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CD CLUSTERED ANTIBODIES
(anti-Human and others as indicated)
NOTE: 10% Discount on purchases of 3 or more any combination of monoclonal CD antibodies. Please inquire for bulk or custom formulations (without preservative or carrier).
RDI Divison of researchd Industries Intl offers a wide line of antibodies. Since no one antibody works best for all applications (flow cytometry, neutralization, blotting, histochemistry, ELISA, etc), we offer many different types of antibodies to help solve this problem. Please inquire for other applications or types of antibodies not listed below. All products are for in vitro research use only-not for use in or on humans or animals-not for use in diagnostics. Price/availability/specifications subject to change without notice.
Mouse anti-human CD59 antibodies
NOTE:You might be interested to know that about a dual labelled protocol for flow cytometry studies of paroxysmal nocturnal haemoglobinurea uses CD59-FITC and CD55PE (our clones MEM43 & 143-30). The authors indicated that the low prevalence of the CD55 required the PE label to be used with that antibody.
The published reference of interest on the CD59FITC/CD55PE is: Practical Haematology (1994) Chapter 15 by Luzzatto & Hillman"Laboratory methods used in the investigation of paraxysomal nocturnal haemoglobinuria".
RDI-CBL467 MEM 43 IV + V mIgG2a purified $438.00
RDI-CBL467FT " " " " FITC $440.00
RDI-CBL467PE " " " " PE $500.00
RDI-CBL467BT " " " " Biotin $438.00
RDI-CBL467PC5 " " " " R-PE/CY5 $500.00
RDI-CBL547 BRA-10G V mIgG2b purified $375.00
RDI-CBL547FT " " " " FITC $375.00
RDI-CBL547PE PE $469.00
RDI-CBL547PC5 RPE-CY5 $469.00
RDI-CBL547BT Biotin $375.00
RDI-CD59-P282 P282 V " purified $312.00
PRODUCT CODE : RDI- CBL 467 $438.00/vial 200ug
Also available:FITC labeled cat#RDI-CBL467FT $375.00/100 Tests
PE labeled cat#RDI-CBL467PE $500.00/100 tests
Biotin labelled cat#RDI-CBL467BT $438.00/100 tests
R-PE/CY5 caT#RDI-CBL467PC5 $500.00/100T
CLONE: MEM 43
SOURCE: Mouse ascitic fluid.
PURIFICATION METHOD: Protein A affinity chromatography.
SPECIFICITY: A Pl-linked glycoprotein, molecular weight 18-20kDa, found in all types of leucocytes including platelets. Antigen distribution is to be found across many haemopoietic and non- haemopoietic cells.
* Identification of CD59 + cells by immunofluorescence and immunoperoxidase methods on cryostat sections.
* Identification of CD59 + cells in formalin fixed paraffin wax embedded sections.
* Diagnosis of paroxysmal nocturnal haemoglobinurea type II & III cells
Leucocyte Typing III, Oxford University Press. (1987).
Leucocyte Typing IV, Oxford University Press. (1989).
Horejsi, V. et al. Fol. Biol. (Prague) 34, 23 - 24 (1988).
Stefanova, I. et al. Mol. Immunol. 26, 153 - 61 (1989).
PRESENTATION: Each monoclonal is delivered in a sealed vial at a concentration of 200ug in 2ml (200T) phosphate buffered saline solution w/1mg/ml BSA & 10mM sodium azide. We recommend that each laboratory determine an optimum working titre for use in its particular application.
STORAGE: Purified monoclonal antibodies are generally stable for long periods at 4oC. However repeated warming to room temperature and re-cooling may result in a loss of activity and hence effective working titre.. DO NOT FREEZE CONJUGATED ANTIBODIES. Dilute working solutions are best prepared on the day of use.
For research use only. Not supplied for use in human diagnostic or therapeutic procedures
Also available in bulk 1mg sizes with or without BSA or azide. Quotations on request (pricing starts under $1188.00/mg for purified material).
PRODUCT CODE:RDI- CBL 547
Also available:FITC labeled cat#RDI-CBL547FT $438.00/100 Tests
PE labeled cat#RDI-CBL547PE $469.00/100 tests
Biotin labelled cat#RDI-CBL547BT $438.00/100 tests
R-PE/CY5 caT#RDI-CBL547PC5 $500.00/100T
SOURCE Tissue culture supernatant
PURIFICATION METHOD Protein A affinity chromatography
SPECIFICITY The antibody reacts with a 19kDa glycosylphosphatidylinositol (GPI) linked glycoprotein expressed on haematopoietic and non-haematopoietic cells.
*Identification of CD59+ cells in formalin fixed paraffin wax embedded sections
*Identification of CD59+ cells by immunofluorescence and immunoperoxidase methods on cryostat sections
*Diagnosis of paroxysmal nocturnal haemoglobinurea type II & III cells
REFERENCES Leucocyte Typing V, Oxford University Press (1995)
The monoclonal is presented at a concentration of 200µg/2ml in phosphate buffered saline containing 10mM sodium azide and 1mg/ml bovine serum albumin. We recommend that each laboratory determine an optimum working titre for use in its particular application.
STORAGE For use within 1 month of purchase store at +4oC, for long term storage aliquot antibody into small volumes and store at -20oC.
For Research Use Only. Not supplied for use in diagnostic or therapeutic procedures.
Catalog#: RDI-CD59-p282 $312.00/vial
biotin labeled cat#RDI-CD59-p282-BT $438.00/100 T vial Bulk on request
Package Size: 100ug purified in 0.2ml (0.PBS pH7.2 with 0.09% sodium azide sodium azide. (sodium azide is toxic, dilute azide compounds with copious amounts of water when disposing in drain to avoid accumulation of explosive deposits- avoid contact with acids.)
Prep: purified by Protein G from TCS
Species: mouse IgG2ak
CLONE: p282 (H19)
Workshop Code: Workshop V, V S006
Activity: -not found to be inhibiting indicating a different epitope is seen than CD59 clone MEM43
Application: -Indirect immunofluorescence cell surface staining. Titer for each application
1) Schlossman, S. et al, ed.s 1995, Leucocyte Typing V, White Cell Differentiation Antigens, Oxford University Press, NY
2) Eur J. Immunol. 22:2943
3) Cellular Immunology 126:176
4) Immunol RES 12:258
Storage: Store at 4 DEG C.
Precautions: For In vitro research Use Only. Not for use in or on humans or animals or for diagnostics. It is the responsibility of the user to comply with all local/state and Federal rules in the use of this product. We are not responsible for any patent infringements that might result with the use of or derivation of this product.
San Jose, 95123 CA Snell ave 658
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