rev: February 20, 2002

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CD CLUSTERED ANTIBODIES  

(anti-Human and others as indicated)

NOTE: 10% Discount on purchases of  3 or more any combination of CD antibodies. Please inquire for bulk or custom formulations (without preservative or carrier).

RDI Divison of researchd Industries Intl  offers a wide line of  antibodies. Since no one antibody works best for all applications (flow cytometry, neutralization, blotting, histochemistry, ELISA, etc), we offer many different types of antibodies to help solve this problem. Please inquire for other applications or types of antibodies not listed below. All products are for in vitro research use only-not for use in or on humans or animals-not for use in diagnostics. Price/availability/specifications subject to change without notice.


CD5 Antibodies


Mouse anti-human CD5 antibodies

-2 clones     CLB-T1/1,1C2 (purified, FITC & PE)

                   UCHT2 (purified, FITC ,PE & R-PE/CY5 )                   


CATALOG#                 CLONE#                Workshop  Host     Form        Price

RDI-M1310clb             CLB-T1/1,1C12            IV      mIgG1   purified    $375.00

RDI-M1602clb             " " "                                "         "           FITC       $375.00

RDI-M1649clb             " "                                  "          "           PE          $500.00

RDI-CBL132              UCHT2                          III      mIgG1    purified   $375.00

RDI-CBL132FT          " " "                                "          "           FITC      $375.00

RDI-CBL132PE          " " "                                "          "           PE          $469.00

RDI-CBL132PC5        "" "                                 "          "          R-PE-CY/5 $469.00

RDI-CD5abm-4C7       4C7                               -          -           1ml          $594.00  (for paraffin)  


Product Specification: Mouse Monoclonal CD5

Code: CLB-CD5 (CLB-T1)

Cat#: RDI-M1310clb


Lot number:


Tests/vial: 200

Specificity: Cell type: Human T lymphocytes.

Ag.MW. : 67 kD.


Reactivity: The monoclonal antibody is directed against the CD5-antigen (T1-antigen), which is expressed on human T lymphocytes. The nonoclonal antibody reacts with 90% of human peripheral T lymphocytes, medullary thymocytes as well as with lymphocytes of patients with chronic B-cell derived leukaemia. The antibody does not react with normal B-cells, monocytes, granulocytes and platelets.


Applications: Monitoring of T-cell numbers inperipheral blood. Characterization of leukaemias and lymphomas. Identification of T-cells in tissue.

Methods: Indirect immunofluorescence staining with analysis by FACS or fluorescence microscopy.

Clone: CLB-157 This clone has been derived from hybridization of SP2/0 cells with spleen cells of a BALB/c mouse immunized with cells of a patient with Sezary Syndrome.


Mouse isotype:The monoclonal antibody is of mouse subclass IgG1.


Source: Ascites fluid of tumour bearing BALB/c mice.


Purification: Ammoniumsulphate precipitation and ion exchange chromatography.


Packing: Each vial contains 1 ml with approximately 0.1mg/ml monoclonal antibody and 10 mg BSA in PBS.

Preservative: Merthiolate (0.001%).

Storage: Monoclonal antibodies should be stored 4 DEG C


FOR IN VITRO RESEARCH USE ONLY


mouse monoclonal anti-human CD5,conjugated to Fluorescein


Code: CLB-CD5-F (CLB-T1)


CAT#: RDI-M1602clb


Specificity: Cell Type: Human T lymphocytes

Ag.MW. : 67 kD.


Reactivity: The monoclonal antibody is directed against the CD-5-antigen (T1-antigen), which is expressed on human T lymphocytes. The monoclonal antibody reacts with 90% of human peripheral T lymphocytes, medullary thymocytes as well as with lymphocytes of patients with chronic B-cell derived leukaemia. The antibody does not react with normal B-cells, monocytes, granulocytes and platelets.


Applications: Monitoring of T-cell numbers in peripheral blood. Characterization of leukaemias and lymphomas. Identification of T-cells in tissue.

Methods: Direct immunofluorescence staining with analysis by FACS or fluorescence microscopy.


Clone: CLB-157 This clone has been derived from hybridization of SP2/0 cells with spleen cells of a BALB/c mouse immunized with cells of a patient with Sezary Syndrome.


Mouse isotype:The monoclonal antibody is of the mouse subclass IgG1.

Source: Ascites fluid of tumor bearing BALB/c mice.


Purification: Ammoniumsulphate precipitation and ion exchange chromatography.


Chemical Characteristics:

Molecular F/P ratio : 6.2

Weight F/P ratio : 16.1 x 10-3

Fluorescein concentration : 4.0 x 10-4 mg/ml


Packing: Each vial contains 1 ml with approximately 25ug/ml conjugated monoclonal antibody and 10 mg BSA in PBS


Preservative: Merthiolate (0.0001%).


Storage: Monoclonal antibodies should be stored 4 DEG C.


FOR IN VITRO RESEARCH USE ONLY


ANTI-Human CD5+ T LYMPHOCYTES & B-CLL CELLS


PRODUCT CODE : RDI-CBL 132   $375.00/vial 200ug   BULK ON REQUEST
also available: FITC conjugated:   cat#RDI-CBL132FT    $375.00/100T
                          PE labeled,           cat#RDI-CBL132PE    $469.00/100 Tests

                          R-PE/CY5            cat#RDI-CBL132PC5  $469.00/100T

CLONE  UCHT2

ISOTYPE  IgG1

SOURCE  Tissue culture supernatent

PURIFICATION METHOD  Protein A affinity chromatography

SPECIFICITY:  This antibody identifies a 67 kDa lymphocyte surface antigen belonging to the CD5 cluster on peripheral blood T lymphocytes, 70% of thymocytes and a limited number of malignancies of T cell origin. The CD5 antigen is expressed on the majority of malignant B CLL, T CLL and T ALL cells and a proportion of B lymphoma cells.


Antigen distribution:

T cells (E+) 100%

Peripheral blood lymphocytes 73 + 6%

Thymocytes 42 + 9%

B Cells (E-) 10 + 5%

Granulocytes < 10%

Monocytes < 10%

NK cells (CD16+) < 1%

APPLICATIONS

*Identification of CD5 positive T lymphocytes and B CLL by indirect immunofluorescence staining

*Leukaemia and lymphoma phenotyping

*Identification of normal and malignant CD5 positive T lymphocytes and malignant B cells in frozen tissue sections

*Isolation or removal of T lymphocytes or B CLL by FACS or panning techniques

REFERENCES   Leucocyte Typing I, Springer-Verlag (1984)

PRESENTATION The monoclonal is presented at a concentration of 200µg/2ml in phosphate buffered saline containing 10mM sodium azide and 1mg/ml bovine serum albumin. We recommend that each laboratory determine an optimum working titre for use in its particular application.

STORAGE For use within 1 month of purchase store at +4oC, for long term storage aliquot antibody into small volumes and store at -20oC.

For Research Use Only. Not supplied for use in diagnostic or therapeutic procedures.

BULK NO AZIDE/NO BSA $1062.00/1 milligram Larger sizes upon request


RDI Divison of researchd Industries Intl

San Jose, 95123 CA Snell ave 658

USA

or 408-780-0908

EMAIL:margaret@cellular-research.com

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