rev: February 9, 2004
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CD CLUSTERED ANTIBODIES
(anti-Human and others as indicated)
NOTE: 10% Discount on purchases of 3 or more any combination of CD antibodies. Please inquire for bulk or custom formulations (without preservative or carrier).
RDI Divison of researchd Industries Intl offers a wide line of antibodies. Since no one antibody works best for all applications (flow cytometry, neutralization, blotting, histochemistry, ELISA, etc), we offer many different types of antibodies to help solve this problem. Please inquire for other applications or types of antibodies not listed below. All products are for in vitro research use only-not for use in or on humans or animals-not for use in diagnostics. Price/availability/specifications subject to change without notice.
RAT anti-human CD120b antibodies
-1 clone see our other TNFR receptor abs (mono's & poly's)
cat# RDI-CD120Babrt $531.00/0.5mg
Presentation: 0.5mg in 1ml (0.5mg/ml) PBS pH7.2 with 150mM NaCl and 0.09% sodium azide
Isotype: Rat IgG2b
Immunogen: COS-expressed recombinant human TNFRII.
Form: purified from tissue culture supernatant via Protein G
DESCRIPTION: The hTNFR-M1 antibody reacts with the extracellular domain of the 75 kD transmembrane receptor for the human cytokines Tumor Necrosis Factor-alpha (TNF-a or TNF) and lymphotoxin-alpha (LT-a 3, aka, lymphotoxin or TNF-ß ).1 This receptor is referred to as the p75 or Type II Tumor Necrosis Factor Receptor (TNFRII) [aka, CD120b]. TNFRII are expressed by the cells of the immune system and hematopoietic cells including macrophages, neutrophils, lymphocytes, thymocytes and mast cells.1-5 TNFRII are expressed by a variety of other cell types including endothelial cells, cardiac myocytes and prostate cells. Naïve B cells express very low or undetectable levels of TNFRII whereas mature erythrocytes and platelets are uniformly negative for TNFRII expression.6-8
STORAGE: The antibody solution should be stored undiluted at 4°C.
Immunofluorescent Staining and Flow Cytometric Analysis. The purified Mab can be used for the immunofluorescent staining (approx 1 µg antibody/1million cells) and flow cytometric analysis of human nucleated cells to measure their expressed levels of surface TNFRII.7,8 A three-layer staining protocol is recommended for maximizing the detection of TNFRII expressed by cells. -NOTE: as a consequence of in vivo or in vitro activation, cell surface TNFRII can either be shed by cells or transiently expressed at higher levels. As a result, cellular activation can affect the cells overall expressed level of surface TNFRII.1,11
Expression of cell surface TNFRII by whole-lysed human blood. Whole human blood was lysed prior to staining with hTNFR-M1. Whole lysed human blood was subsequently blocked with normal polyclonal human IgG and stained with purified hTNFR-M1 (0.06µg/10 6 cells) followed by biotinylated goat anti-rat F(ab')2 IgG and streptavidin hycoerythrin (0.015 µg. Staining with the hTNFR-M1 antibody is compared to staining obtained using the isotype control antibody Gated events with the light scattering characteristics of viable lymphocytes (A), monocytes (B) and granulocytes (C). Note: Certain human cell lines or cell types (e.g., neutrophils, monocytes) can first be treated with reagents that block receptors for the Fc regions of immunoglobulin to avoid nonspecific immunofluorescent staining mediated by Fc receptors (for example see References 1 and 12).
Investigators are advised to determine optimal concentrations for individual applications.
1. Ware, C. F., P. D. Crowe, VanArsdale, T. L., J. L. Andrews, M. H. Grayson, R. Jerzy, C. A. Smith, and R. G. Goodwin. 1991. Tumor necrosis factor (TNF) receptor expression in T lymphocytes. Differential regulation of the type I TNF receptor during activation of resting and effector T cells. J Immunol 147:4229-38.
2. Gehr, G., R. Gentz, M. Brockhaus, H. Loetscher, and W. Lesslauer. 1992. Both tumor necrosis factor receptor types mediate proliferative signals in human mononuclear cell activation. J. Immunol. 149:911-917.
3. Hohmann, H. P., R. Remy, M. Brockhaus, and A. P. van Loon. 1989. Two different cell types have different major receptors for human tumor necrosis factor (TNF alpha). J. Biol. Chem. 264:14927-14934.
4. Brockhaus, M., H. J. Schoenfeld, E. J. Schlaeger, W. Hunziker, W. Lesslauer, and H. Loetscher. 1990. Identification of two types of tumor necrosis factor receptors on human cell lines by monoclonal antibodies. Proc. Natl. Acad. Sci. USA 87:3127-3131.
5. Aggarwal, S., S. Gollapudi, and S. Gupta. 1999. Increased TNF-alpha-induced apoptosis in lymphocytes from aged humans: changes in TNF-alpha receptor expression and activation of caspases. J. Immunol. 162:2154-2161.
6. Erikstein, B. K., E. B. Smeland, H. K. Blomhoff, S. Funderud, K. Prydz, W. Lesslauer, and T. Espevik. 1991. Independent regulation of 55-kDa and 75-kDa tumor necrosis factor receptors during activation of human peripheral blood B lymphocytes. Eur. J. Immunol. 21:1033-1037.
7. Munker, R., J. DiPersio, and H. P. Koeffler. 1987. Tumor necrosis factor: receptors on hematopoietic cells. Blood 70:1730.
8. Heilig, B., M. Mapara, M. Brockhaus, K. Krauth, and B. Dorken. 1991. Two types of TNF receptors are expressed on human normal and malignant B lymphocytes. Clin Immunol Immunopathol 61:260-267.7. Munker, R., J. DiPersio, and H. P. Koeffler. 1987. Tumor necrosis factor: receptors on hematopoietic cells. Blood 70:1730-1734.
9. Zola, H. 1995. Detection of cytokine receptors by flow cytometry. In Current Protocols in Immunology . J. E. Coligan, A. M. Kruisbeek, D. H. Margulies, E. M. Shevach, and W. Strober, eds. Greene Publishing Associates and Wiley-Interscience, New York, p. 6.21.1-6.21.18.
10. Zola, H. 1994. Detection of receptors for cytokines and growth factors. The Immunologist 2:47-50.
11. Wallach, D., H. Engelmann, Y. Nophar, D. Aderka, O. Kemper, V. Hornik, H. Holtmann, and C. Brakebusch. 1991. Soluble and cell surface receptors for tumor necrosis factor. Agents Actions Suppl. 35:51-57.
12. Browning, J. L., I. Dougas, A. Ngam-ek, P. R. Bourdon, B. N. Ehrenfels, K. Miatkowski, M. Zafari, A. M. Yampaglia, P. Lawton, W. Meier, C. P. Benjamin, and C. Hession. 1995. Characterization of surface lymphotoxin forms. Use of specific monoclonal antibodies and soluble receptors. J. Immunol. 154:33-46.
Precautions: For In vitro research Use Only. Not for use in or on humans or animals or for diagnostics.
San Jose, 95123 CA Snell ave 658
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