rev:       May 26, 2005

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(anti-Human and others as indicated)

RDI Divison of researchd Industries Intl  offers a wide line of  antibodies. Since no one antibody works best for all applications (neutralization, blotting, ELISA, etc), we offer many different types of antibodies to help solve this problem. Please inquire for other applications or types of antibodies not listed below.

Neurofilament NF-H Monoclonal Antibody

Catalog Number:  RDI-ALS24505     $344.00/100ul

Clone: NAP4

Quantity: 100ul

Description: Neurofilaments are the 10nm or intermediate filament proteins found specifically in neurons, and are composed predominantly of three major proteins called NF-L, NF-M and NF-H. NF-H is the neurofilament high or heavy molecular weight polypeptide and runs on SDS-PAGE gels at 200-220 kDa, with some variability across species boundaries. Antibodies to NF-H are useful for identifying neuronal cells and their processes in tissue sections and in tissue culture. NF-H antibodies can also be useful in the diagnostics of neurofilament accumulations seen in many neurological diseases, such as Amyothrophic lateral sclerosis (also known as Lou Gehrig's disease) and Alzheimer's disease. NAP4 is one of numerous antibodies which reacts preferentially with the axonal phosphorylated forms of NF-H. Interestingly these phosphorylated forms of NF-H are normally restricted to axons, while less phosphorylated forms are found in dendrites. However in numerous damage and disease states, phosphorylated NF-H can be detected with NAP4 in dendritic and perikaryal neurofilaments.

Host Species: Mouse

Specificity: NAP4 is a mouse monoclonal antibody raised against a preparation of native NF-H purified from pig spinal cord. Recognizes phosphorylated NF-H KSP (lysine-serine-proline) type sequences. In some species there is some cross-reactivity with the related phosphorylated KSP sequences found in the related neurofilament subunit NF-M. The antibody recognizes NF-H strongly in all mammals tested to date and also in chicken. It recognizes neurofilaments in frozen sections in tissue culture and in formalin fixed sections.

Applications: For immunofluorescence use dilutions of 1:500. For immunohistochemistry with ABC or other enzymatic amplification procedures use 1:5,000. For western blots use 1:10,000 dilutions.

Format: Sterile cell culture fluid plus sodium azide. NAP4 is a mouse IgG1 class antibody with a k light chain. Supplied at a concentration of 1mg/ml.

Storage: Maintain at +2-8°C for 3 months or at -20°C for longer periods. Stable for 1 year. Avoid repeated freeze-thaw cycles.

Reference: 1. Harris, J., Ayyub, C. and Shaw G. A molecular dissection of the carboxyterminal tails of the major neurofilament subunits NF-M and NF-H. J Neurosci Res 30:47-62 1991.

For research use only; not for use as a diagnostic.

For research use only; not for use as a diagnostic.

RDI Div of researchd Industries Intl

(Research Diagnostics Inc)

San Jose, 95123 CA Snell ave 658


or 408-780-0908


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