rev:  February 22, 2002

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  VIRAL ANTIGENS/Pure Proteins 

(for in vitro research use only)

RDI Divison of Fitzgerald Industries Intl  offers a wide line of  antigens for use in histochemistry, ELISA, and further manufacturing use. Please inquire for other antigens or antibodies to use with these products. Please inquire for Bulk quantities-significant discounts available!

Antigen Data Sheet:    HIV-1 gp 41-1 Antigen

Cat# RDI-RHIV1AG      $469.00/100 micrograms

                                      $875.00/1MG      $750.00/mg 3 or more

                        Significant Bulk-Please Request Quotations on 25mg or more

Grade: recombinant human

Lot#:

Source: Glycosylated recombinant ecto-domain of gp 41 (amino acids 546-682, HxB2) produced in pichia pastoris(without the membrane spanning and cytoplasmic domains). The protein is heterogeneously glycosylated and is seen predominantly as a series of bands from 20,000 to 30,000 daltons on Western blot.

Reactivity: Reacts with Aids sera and human monoclonals specific for conformational and linear epitopes as determined by ELISA/Western blot. Polyhistidine is immunologically unreactive.

Form: 6M Urea, 0.5M NaCl, 0.02M sodium phosphate, 0.2 wt% Triton X-100 assay

Characteristic Specification lot#SAMPLE
Appearance colorless to light tan clear solution  +
pH    pH7.0-8.0  7.43
MW by SDS-PAGE Three major bands migrating between 20 and 30kDa.

Minor bands between 20 and 30kDa and at approx 14

and approx 7kda.Aggregation smear at approx

35kda and greater. Comparable   to reference lot.

 Meets Spec
Protein concentration

Coomassie(tm) Plus

 Greater than or equal by 1mg/ml +- 10% 1.0 +- 10%

(See actual with each shipment)

Biohazard: None identified for recombinant material

Storage: -70'C or colder. Avoid frequent freeze thaw cycles. Store in working aliquots.

FOR INVITRO Research or Further Manufacturing USE ONLY-NOT FOR USE IN OR ON

HUMANS OR ANIMALS-NOT FOR USE IN DIAGNOSTICS


Caution: not for use in humans or clinical diagnosis: This product is intended for research or manufacturing use only.It is pharmaceutically unrefinded, and verification of its suitability for use in humans or as clinical diagnostic reagents and the compliance with all Federal and State laws regulating such applications are the responsibility of the purchaser. There is no expressed or implied warranty. No liability is assumed for fitness of purpose and merchantability, or direct or consequential damages. The user assumes all responsibility for care,custody and control of the material, including its disposal, in accordance with all regulations.

RDI Divison of Fitzgerald Industries Intl

34 Junction Square Drive

Concord MA 01742-3049

USA

phone (978) 371-6446 or (800) 370-2222

fax     (978) 371-2266

EMAIL:antibodies@fitzgerald-fii.com

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