rev: January 17, 2005
HOME (home page)
Return (viral antigen.index)
VIRAL
ANTIGENS/Pure
Proteins
(for in vitro research use only)
RDI Divison of Fitzgerald Industries Intl offers a wide line of antigens for use in histochemistry, ELISA, and further manufacturing use. Please inquire for other antigens or antibodies to use with these products. Please inquire for Bulk quantities-significant discounts available!
-also new natural isolates for CMV IgG and IgM assays
-$250.00/100ug $625.00/0.5mg $1125.0/1mg Bulk quotes on request
RDI-CMV 211AG Recombinant Cytomegalo Virus gB (11-76 a.a.)
RDI-CMV212AG Recombinant Cytomegalo Virus pP28 (UL99) (130-160 a.a.)
RDI-CMV213AG Recombinant Cytomegalo Virus Pp38 (UL80a) (117-373 a.a.)
RDI-CMV214AG Recombinant Cytomegalo Virus pP52 (UL44) (202-434 a.a.)
RDI-CMV215AG Recombinant Cytomegalo Virus pP65(UL83) (297-510 a.a.), 52,282 Dalton
RDI-CMV216AG Recombinant Cytomegalo Virus pP150(UL32) (1011-1048 a.a.)
Cat# RDI-CMV211AG $625.00/500 µg
PRODUCT DESCRIPTION: E.coli derived recombinant.
The protein contains the CMV gB immunodominant regions.
PURIFICATION METHOD: Purified by proprietary chromatographic technique.
PURITY: Protein is >95% pure as determined by 10% PAGE (coomassie staining).
PROTEIN CONC: 1mg/ml in 25mM Tris-Hcl pH 8.0, 1mM EDTA and 50% glycerol.
STORAGE: Protein is shipped at ambient temperature. Upon arrival, Store at -20 DEG C.
STABILITY: Five years frozen. One month in solution at room temperature.
SPECIFICITY: Immunoreactive with sera of CMV-infected individuals.
APPLICATIONS: Antigen in ELISA and Western blots, excellent antigen for detection of CMV with minimal specificity problems.
Precautions: For In vitro research Use Only. Not for use in or on humans or animals or for diagnostics. It is the responsibility of the user to comply with all local/state and Federal rules in the use of this product. We are not responsible for any patent infringements that might result with the use of or derivation of this product.
Cat# RDI-CMV212AG
PRODUCT DESCRIPTION: E.coli derived recombinant.
The protein contains the CMV Pp28(UL99) immunodominant regions.
PURIFICATION METHOD: Purified by proprietary chromatographic technique.
PURITY: Protein is >95% pure as determined by 10% PAGE (coomassie staining).
PROTEIN CONC: 1mg/ml in 25mM Tris-Hcl pH 8.0, 1mM EDTA and 50% glycerol.
STORAGE: Protein is shipped at ambient temperature. Upon arrival, Store at -200C.
STABILITY: Five years frozen. One month in solution at room temperature.
SPECIFICITY: Immunoreactive with sera of CMV-infected individuals.
APPLICATIONS: Antigen in ELISA and Western blots, excellent antigen for detection of CMV with minimal specificity problems.
Precautions: For In vitro research Use Only. Not for use in or on humans or animals or for diagnostics. It is the responsibility of the user to comply with all local/state and Federal rules in the use of this product. We are not responsible for any patent infringements that might result with the use of or derivation of this product.
Cat# RDI-CMV213AG
PRODUCT DESCRIPTION: E.coli derived recombinant.
The protein contains the CMV Pp38(UL80a) immunodominant regions.
PURIFICATION METHOD: Purified by proprietary chromatographic technique.
PURITY: Protein is >95% pure as determined by 10% PAGE (coomassie staining).
PROTEIN CONC: 1mg/ml in 25mM Tris-Hcl pH 8.0, 1mM EDTA and 50% glycerol.
STORAGE: Protein is shipped at ambient temperature. Upon arrival, Store at -200C.
STABILITY: Five years frozen. One month in solution at room temperature.
SPECIFICITY: Immunoreactive with sera of CMV-infected individuals.
APPLICATIONS: Antigen in ELISA and Western blots, excellent antigen for detection of CMV with minimal specificity problems.
Precautions: For In vitro research Use Only. Not for use in or on humans or animals or for diagnostics. It is the responsibility of the user to comply with all local/state and Federal rules in the use of this product. We are not responsible for any patent infringements that might result with the use of or derivation of this product.
Cat# RDI-CMV214AG
PRODUCT DESCRIPTION: E.coli derived recombinant.
The protein contains the CMV Pp52 (UL44) immunodominant regions.
PURIFICATION METHOD: Purified by proprietary chromatographic technique.
PURITY: Protein is >95% pure as determined by 10% PAGE (coomassie staining).
PROTEIN CONC: 1mg/ml in 25mM Tris-Hcl pH 8.0, 1mM EDTA and 50% glycerol.
STORAGE: Protein is shipped at ambient temperature. Upon arrival, Store at -200C.
STABILITY: Five years frozen. One month in solution at room temperature.
SPECIFICITY: Immunoreactive with sera of CMV-infected individuals.
APPLICATIONS: Antigen in ELISA and Western blots, excellent antigen for detection of CMV with minimal specificity problems.
Precautions: For In vitro research Use Only. Not for use in or on humans or animals or for diagnostics. It is the responsibility of the user to comply with all local/state and Federal rules in the use of this product. We are not responsible for any patent infringements that might result with the use of or derivation of this product.
Cat# RDI-CMV215AG
PRODUCT DESCRIPTION: E.coli derived recombinant.
The protein contains the CMV Pp65(UL83) immunodominant regions.
QUANTITY: 1mg
LOT#:
PURIFICATION METHOD: Purified by proprietary chromatographic technique.
PURITY: Protein is >95% pure as determined by 10% PAGE (coomassie staining).
PROTEIN CONC: 1mg/ml in 25mM Tris-Hcl pH 8.0, 1mM EDTA and 50% glycerol.
STORAGE: Protein is shipped at ambient temperature. Upon arrival, Store at -200C.
STABILITY: Five years frozen. One month in solution at room temperature.
SPECIFICITY: Immunoreactive with sera of CMV-infected individuals.
APPLICATIONS: Antigen in ELISA and Western blots, excellent antigen for detection of CMV with minimal specificity problems.
Precautions: For In vitro research Use Only. Not for use in or on humans or animals or for diagnostics. It is the responsibility of the user to comply with all local/state and Federal rules in the use of this product. We are not responsible for any patent infringements that might result with the use of or derivation of this product.
Cat# RDI-CMV216AG
PRODUCT DESCRIPTION: E.coli derived recombinant.
The protein contains the CMV Pp150 (UL32) immunodominant regions.
PURIFICATION METHOD: Purified by proprietary chromatographic technique.
PURITY: Protein is >95% pure as determined by 10% PAGE (coomassie staining).
PROTEIN CONC: 1mg/ml in 25mM Tris-Hcl pH 8.0, 1mM EDTA and 50% glycerol.
STORAGE: Protein is shipped at ambient temperature. Upon arrival, Store at -200C.
STABILITY: Five years frozen. One month in solution at room temperature.
SPECIFICITY: Immunoreactive with sera of CMV-infected individuals.
APPLICATIONS: Antigen in ELISA and Western blots, excellent antigen for detection of CMV with minimal specificity problems.
Precautions: For In vitro research Use Only. Not for use in or on humans or animals or for diagnostics. It is the responsibility of the user to comply with all local/state and Federal rules in the use of this product. We are not responsible for any patent infringements that might result with the use of or derivation of this product.
cat#RDI-CMVGSOL-AG $312.00/1ml + $125/ml 2 or more $1562.00/10ml Bulk quotes on request
Viral Strain: AD169 from ATCC, propagated in HF Cells , packaged in 0.1 M Glycine, pH 9.5 +/- 0.2
approx 0.15 - 0.25 mg/ml (<10% Viral Protein) see batch sheet for batch specific information Store -70 DEG C
-recommended for IgG antibody detection
cat#RDI-CMVMSOL-AG $312.00/1ml + $125/ml 2 or more $1562.00/10ml Bulk quotes on request
Viral Strain: AD169 from ATCC, propagated in HF Cells , packaged in 0.1 M Glycine, pH 9.5 +/- 0.2
approx 0.25 - 0.35 mg/ml (<10% Viral Protein) see batch sheet for batch specific information Store -70 DEG C
-recommended for IgM antibody detection
Custom Packaging is available
Quality Control involves coating ELISA plates and assaying a series of panel
samples. Reactivity to the negative control must not be more than twice the
blank. Infectivity Analysis: Negative infectivity for CMV is defined as the
inability of an inactivated virus preparation to produce CPE after eighteen
(18) days of incubation on a monolayer of HF cells.
Cytomegalovirus (CMV) is considered one of the most common viral infections
in man. The virus rarely causes disease, but may persist as a latent or chronic
infection of the salivary glands, renal tubules, cervix and leukocytes. The
incidence and spectrum of the disease in newborns and immunocompromised hosts
establish the virus as an important human pathogen. CMV infections are frequent
and occasionally severe in children or adults with congenital or acquired
defects of cellular immunity, such as patients with AIDS, cancer patients,
and recipients of organ transplants. The transmission of CMV via transfused
blood may account for most CMV infections in organ transplant recipients,
neonates, and heart surgery patients. Medical conditions caused by CMV include:
interstitial pneumonia, mononucleosis (heterophile negative), congenital
defects, abortion, mental retardation, blindness and deafness.
Serological tests for the detection and quantification of antibodies to CMV
are a valuable aid in diagnosing primary infection. Serology represents the
only practical system at present for screening blood and organ donors and
recipients to prevent the transmission of latent CMV to patients at high
risk for severe CMV disease.
Cytomegalovirus Antigens are prepared by proprietary glycine extraction of
human fibroblast cells infected with cytomegalovirus, strain AD 169 (ATCC).
The CMV-M extraction procedure differs from the CMV-G procedure; it is designed
to increase the relative amount of early viral CMV antigens. Both antigen
preparations are partially purified to reduce host cell components prior
to inactivation with UV light. The CMV-G & CMV-M antigens have yielded
lot-to-lot consistency in IgG & IgM ELISA tests respectively, providing
high sensitivity and low backgrounds.
Higher antibody reactivity is achieved with lower total protein for both the CMV-G and CMV-M products as compared to two commercial products. The higher specific activity achieved through partial purification of the CMV antigens assures improved sensitivity and specificity for your assays.
34 Junction Square Drive
Concord MA 01742-3049
USA
phone (978) 371-6446 or (800) 370-2222
fax (978) 371-2266
EMAIL:antibodies@fitzgerald-fii.com
Return (viral antigen.index)
Ordering terms