rev:   January 20, 2005

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  VIRAL ANTIGENS/Pure Proteins 

(for in vitro research use only)

RDI Division of Fitzgerald Industries Intl  offers a wide line of  antigens for use in histochemistry, ELISA, and further manufacturing use. Please inquire for other antigens or antibodies to use with these products. Please inquire for Bulk quantities-significant discounts available!

           $250.00/100ug     $625.00/500ug     $1125.00/1mg   Bulk quotes on request

RDI-RUB291AG    Recombinant Rubella Virus E1 Mosaic (157-176, 374-390, 213-239a.a.)

RDI-RUB292AG   Recombinant Rubella Virus E2 (31-105 a.a.)  

RDI-RUB293AG   Recombinant Rubella Virus Capsid C (1-123a.a.)

Antigen Data Sheet: recombinant Rubella Virus Antigen

Cat# RDI-TRK8RV78     $500.00/100ug     $1125.00/1mg    Bulk quotes on request

Bulk quotations on Request

Grade: recombinant antigen is a virus-like particle (VLP) containing all the three structural rubella virus proteins, namely c-core protein and 2 surface glycoproteins E1 and E2 but lacking genomic viral RNA.

Source: recombinant antigen is produced by continuous cell line of an animal origin transfected by plasmid bearing the set of rubella virus structural protein genes.

Application: Coating/capture antigen for specific IgG/IgM antibody rubella virus detection in human sera

Purification: The recombinant cell culture is grown using alpha-MEM containing 10% FCS by roller cultivation. After reaching a continuous monolayer a tissue culture is harvested and clarified by a slow spped centrifugation at 1000 rpm for 30 minutes. VLP are pelleted by high- speed centrifugation and further purified by sucrose density 30-60% linear gradietn centrifugation at 25000 rpm for 2.5 hours. The VLP zone is collected, diluted ten times with PBS and pelleted by centrifugation at 21000 rpm for 1.5 hours.

Presentation: Solution in PBS, pH7.2 containing 0.1mM PMSF

Protein conc: 1mg/ml by modified Bradford method (Bio-Rad Protein Assay Kit II, cat> 500-0002)

Immunoreactivity: Tested in EIA and western blotting using anti-rubella Mab clone Ru6 (see cat#RDI-TRK3R23-Ru6) and anti- Rubella virus positive human sera.

Storage: -20'C


For In Vitro Research or Further Manufacturing Use Only

RDI Division of Fitzgerald Industries Intl

34 Junction Square Drive

Concord MA 01742-3049

USA

phone (978) 371-6446 or (800) 370-2222

fax     (978) 371-2266

EMAIL:antibodies@fitzgerald-fii.com

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