rev: February 15, 1999 (revised clone data)

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ANTIBODIES  

(anti-Human and others as indicated)

RDI Divison of Fitzgerald Industries Intl  offers a wide line of  antibodies. Since no one antibody works best for all applications (neutralization, blotting, histochemistry, ELISA, etc), we offer many different types of antibodies to help solve this problem. Please inquire for other applications or types of antibodies not listed below.

Monoclonal mouse anti-A,B, D group specific Salmonella O-antigens

CAT#: RDI-TRK3SO22- clone#

PRICE: $375.00/mg  $312.00/mg 2-4mg   $250.00/mg 5-9

Clone: Group A: 10B10G,

           Group B: 8C11,C

           Group D: 4G7C

            A,B,D group: 10D9H

            Pan Salmonella:5D12A

These clones were derived from hybridization of SP2/0 myeloma cells with spleen cells of a Balb/c mice immunized with heat inactivated bacterial cells of Salmonella paratyphi (Gourp A), Salmonella typhimurium (group b) and Salmonella enbteriditis (group d).

Specificity: see reactivity table

Serogroup CLONE 1 2 3 4 5 6 7 8 9 10 AG Determinant
A 10B10G + - - - - - - - - - 0-2
Pan 5D12A + + + + + + + Core
B 8C11C - + - - - - - - - - 0-4
D 4G7C - - + - - - - - - - 0-9
A,B,D 10D9H + + + - - +- +- - - - 0-12

1-Salmonella paratyphi, 2-Salmonella typhimurium, 3-Salmonella enteriditis, 4-Salmonella cholerase, 5-Salmonella newport 6-Salmonella anatum, 7-Salmonella Selandia, 8-E. Coli 055:B5, 9-E. Coli K12, 10-Klebsiella pneum,

Mouse isotype: mIgG1

Purification: Purified by chromatography on protein A Sepharose. Purity is tested by electrophoresis.

Binding Constants (Ka,M-1) of monoclonal antibodies with LPS of A,B,D groups as determined by the method of Beatty et a.:

CLONE Serogroup LPS A LPS B LPS D LPD E
10B10G A 2 X 10 E07 - - -
10D9H A,B,D 2.1 X 10 E09 1 .1 X 10 E09 6.5 X 10 E08 1 X 10 E06
4G7C D - - 2 X 10 E07 -
5D12A Pan 1 X 10 E09 1 X 10 E07 1X 10 E07 1 X 10 E10

Presentation: Each vial contains 1mg of antibodies with concentration mg/ml, in PBS, pH 7.4, containing 0.1% of sodium azide as preservative. Store at 4 DEG C.

Material safety note: This product is sold as an antibody preparation for research purpose only. Standard Laboratory Practices should be followed when handling this material. Contains sodium azide (0.1%) as preservative. Although the amount of sodium azide is very small appropriate care must be taken when handling this product.

For in Vitro Research Use Only

RDI Divison of Fitzgerald Industries Intl

34 Junction Square Drive

Concord MA 01742-3049

USA

phone (978) 371-6446 or (800) 370-2222

fax     (978) 371-2266

EMAIL:antibodies@fitzgerald-fii.com

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