rev: February 15, 1999 (revised clone data)
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ANTIBODIES
(anti-Human and others as indicated)
RDI Divison of researchd Industries Intl offers a wide line of antibodies. Since no one antibody works best for all applications (neutralization, blotting, histochemistry, ELISA, etc), we offer many different types of antibodies to help solve this problem. Please inquire for other applications or types of antibodies not listed below.
CAT#: RDI-TRK3SO22- clone#
PRICE: $375.00/mg $312.00/mg 2-4mg $250.00/mg 5-9
Clone: Group A: 10B10G,
Group B: 8C11,C
Group D: 4G7C
A,B,D group: 10D9H
Pan Salmonella:5D12A
These clones were derived from hybridization of SP2/0 myeloma cells with spleen cells of a Balb/c mice immunized with heat inactivated bacterial cells of Salmonella paratyphi (Gourp A), Salmonella typhimurium (group b) and Salmonella enbteriditis (group d).
Specificity: see reactivity table
Serogroup | CLONE | 1 | 2 | 3 | 4 | 5 | 6 | 7 | 8 | 9 | 10 | AG Determinant |
A | 10B10G | + | - | - | - | - | - | - | - | - | - | 0-2 |
Pan | 5D12A | + | + | + | + | + | + | + | Core | |||
B | 8C11C | - | + | - | - | - | - | - | - | - | - | 0-4 |
D | 4G7C | - | - | + | - | - | - | - | - | - | - | 0-9 |
A,B,D | 10D9H | + | + | + | - | - | +- | +- | - | - | - | 0-12 |
1-Salmonella paratyphi, 2-Salmonella typhimurium, 3-Salmonella enteriditis, 4-Salmonella cholerase, 5-Salmonella newport 6-Salmonella anatum, 7-Salmonella Selandia, 8-E. Coli 055:B5, 9-E. Coli K12, 10-Klebsiella pneum,
Mouse isotype: mIgG1
Purification: Purified by chromatography on protein A Sepharose. Purity is
tested by electrophoresis.
Binding Constants (Ka,M-1) of monoclonal antibodies with LPS of A,B,D groups as determined by the method of Beatty et a.:
CLONE | Serogroup | LPS A | LPS B | LPS D | LPD E |
10B10G | A | 2 X 10 E07 | - | - | - |
10D9H | A,B,D | 2.1 X 10 E09 | 1 .1 X 10 E09 | 6.5 X 10 E08 | 1 X 10 E06 |
4G7C | D | - | - | 2 X 10 E07 | - |
5D12A | Pan | 1 X 10 E09 | 1 X 10 E07 | 1X 10 E07 | 1 X 10 E10 |
Presentation: Each vial contains 1mg of antibodies with concentration mg/ml,
in PBS, pH 7.4, containing 0.1% of sodium azide as preservative. Store at
4 DEG C.
Material safety note: This product is sold as an antibody preparation for
research purpose only. Standard Laboratory Practices should be followed when
handling this material. Contains sodium azide (0.1%) as preservative. Although
the amount of sodium azide is very small appropriate care must be taken when
handling this product.
For in Vitro Research Use Only
San Jose, 95123 CA Snell ave 658
USA
or 408-780-0908
EMAIL:margaret@cellular-research.com
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