rev: June 20, 2003
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ANTIBODIES
(anti-Human and others as indicated)
RDI Divison of Fitzgerald Industries Intl offers a wide line of antibodies. Since no one antibody works best for all applications (neutralization, blotting, histochemistry, ELISA, etc), we offer many different types of antibodies to help solve this problem. Please inquire for other applications or types of antibodies not listed below.
CAT#: RDI-TRK3RE21-8C5 and RDI-TRK3RE21-9C5 or RDI-TRK3RE21-8B10
PRICE: $375.00/mg $344.00/mg 2-9mg $281.00/mg 10+ Bulk on request
Clone: 8C5 & 9C5 or 8B10
These clones have been derived from the hybridization of Sp2/0 myeloma cells
with spleen cells of a Balb/c mice immunized with purified RSV virus, strain
Long
Specificity: Two clones out of this panel 8C5 and 9C5 we recommend for
immunoassay. In Western blot Mab 8C5 reacts with protein, having Mr 90 K,
that corresponds in mobility to protein G. Mab 9C5 specificity was determined
by competitive ELISA with Mab 131-2A and 92-11C (CDC, Atlanta): they are
directed to the same F1a epitope, localized on F-protein. Mab 8C5 and 9C5
may be used in sandwich ELISA for RSV detection both with themselves and
in a mixed combination, taking into account that 9C5 is especially suitable
for conjugation.
Mab 8C5 and 9C5 have virus-neutralizing activity, 8C5 block RSV-target cells binding, 9C5 hampers the virus penetration into the cell.
Clone 8B10 can be used for detection of incomplete virus assembly. Antibodies of clone 8B10 are directed to N protein, which belongs to nucleoproteid complex of virus. Epitope of this antibody is very unstable. The Mab is failing to recognize the N-protein epitope of immature and destroyed virions, which indicates a conformation dependence of the 8B10 binding site. It reacts well only with mature virison with completely assembled nucelocapsids.
Mouse isotype: mIgG2b (9C5 or 8C5), mIgG1 (8B10)
Application: Detection of RSV
Purification: Purified by chromatography on protein A Sepharose. Purity is
tested by electrophoresis.
Presentation: Each vial contains 1mg of antibodies with concentration mg/ml,
in PBS, pH 7.4, containing 0.1% of sodium azide as preservative. Store at
4 DEG C.
Material safety note: This product is sold as an antibody preparation for
research purpose only. Standard Laboratory Practices should be followed when
handling this material. Contains sodium azide (0.1%) as preservative. Although
the amount of sodium azide is very small appropriate care must be taken when
handling this product.
For in Vitro Research Use Only
Cross-reactivity pattern of Monoclonal antibodies against respiratory viruses
D 492
34 Junction Square Drive
Concord MA 01742-3049
USA
phone (978) 371-6446 or (800) 370-2222
fax (978) 371-2266
EMAIL:antibodies@fitzgerald-fii.com
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