rev:    June 20, 2003

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ANTIBODIES  

(anti-Human and others as indicated)

RDI Divison of Fitzgerald Industries Intl  offers a wide line of  antibodies. Since no one antibody works best for all applications (neutralization, blotting, histochemistry, ELISA, etc), we offer many different types of antibodies to help solve this problem. Please inquire for other applications or types of antibodies not listed below.


Monoclonal mouse anti-Respiratory Syncytial virus (RSV)

CAT#: RDI-TRK3RE21-8C5   and    RDI-TRK3RE21-9C5 or RDI-TRK3RE21-8B10

PRICE: $375.00/mg   $344.00/mg 2-9mg     $281.00/mg 10+  Bulk on request


Clone: 8C5 & 9C5 or 8B10

These clones have been derived from the hybridization of Sp2/0 myeloma cells with spleen cells of a Balb/c mice immunized with purified RSV virus, strain Long

Specificity: Two clones out of this panel 8C5 and 9C5 we recommend for immunoassay. In Western blot Mab 8C5 reacts with protein, having Mr 90 K, that corresponds in mobility to protein G. Mab 9C5 specificity was determined by competitive ELISA with Mab 131-2A and 92-11C (CDC, Atlanta): they are directed to the same F1a epitope, localized on F-protein. Mab 8C5 and 9C5 may be used in sandwich ELISA for RSV detection both with themselves and in a mixed combination, taking into account that 9C5 is especially suitable for conjugation.

Mab 8C5 and 9C5 have virus-neutralizing activity, 8C5 block RSV-target cells binding, 9C5 hampers the virus penetration into the cell.

Clone 8B10 can be used for detection of   incomplete virus assembly. Antibodies of clone   8B10 are directed to N protein, which belongs to   nucleoproteid complex of virus. Epitope of this   antibody is very unstable. The Mab is failing to   recognize the N-protein epitope of immature and   destroyed virions, which indicates a conformation dependence of the 8B10 binding site. It reacts   well only with mature virison with completely assembled nucelocapsids.



Mouse isotype: mIgG2b (9C5 or 8C5), mIgG1 (8B10)

Application: Detection of RSV

Purification: Purified by chromatography on protein A Sepharose. Purity is tested by electrophoresis.

Presentation: Each vial contains 1mg of antibodies with concentration mg/ml, in PBS, pH 7.4, containing 0.1% of sodium azide as preservative. Store at 4 DEG C.

Material safety note: This product is sold as an antibody preparation for research purpose only. Standard Laboratory Practices should be followed when handling this material. Contains sodium azide (0.1%) as preservative. Although the amount of sodium azide is very small appropriate care must be taken when handling this product.

For in Vitro Research Use Only


FIGURE 1

Cross-reactivity pattern of Monoclonal antibodies against respiratory viruses

D 492


RDI Divison of Fitzgerald Industries Intl

34 Junction Square Drive

Concord MA 01742-3049

USA

phone (978) 371-6446 or (800) 370-2222

fax     (978) 371-2266

EMAIL:antibodies@fitzgerald-fii.com

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