rev:  February 9, 2004

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ANTIBODIES  

(anti-Human and others as indicated)

RDI Divison of Fitzgerald Industries Intl  offers a wide line of  antibodies. Since no one antibody works best for all applications (neutralization, blotting, histochemistry, ELISA, etc), we offer many different types of antibodies to help solve this problem. Please inquire for other applications or types of antibodies not listed below.


PRODUCT DATA SHEET  see also clone FB11
Monoclonal mouse anti-LPS Fr. tularensis


CAT#: RDI-TRK3FT6-T14

PRICE: $375.00/1mg or     $344.00/mg   2 or more

Clone: T14

This clone has been derived from hybridization of Sp2/0 myeloma cells with spleen cells of a balb/c mice immunized with pooled water-soluble antigens from disintegrated microbial mass of Fr.tularensis vaccine strain.|

Reactivity: MoAb T14 react with LPS of Fr.tularensis. There is no cross-reactivity with Y pestis, Y. pseudotuberculosis, Y. enterocolitica, V. cholera, E.Coli, S. typhimurium, Fr. novicida, Br, melitensis, Br. abortus, Br.suis,Br.ovis, Br.neotomae. Indirect ELISA titre using purified LPS Fr.tularensis as a coating antigen 1:65 000.

Mouse isotype: IgG3

Application: MoAb T14 can be used for detection of Fr.tularensis in ELISA and immunofluorescence technique.

Purification: Purified by chromatography on protein A Sepharose. Purity is tested by electrophoresis.

Presentation: Each vial contains 1 ml of antibodies with concentration 1 mg/ml, in PBS, pH 7.4, containing 0.1% of sodium azide as preservative.Store at -20C. Avoid repeated freeze-thaw cycles.


Material Safety Note: This product is sold as an antibody preparation for research purpose only. Standard Laboratory Practices should be followed when handling this material. Contains sodium azide (0.1%) as preservative. Although the amount of sodium azide is very small appropriate care must be taken when handling this product.


FOR IN VITRO RESEARCH USE ONLY


Monoclonal mouse anti-LPS Fr. tularensis  cloen FB11


CAT#: RDI-TRK3FT6-FB11   PRICE: $375.00/mg  or     $344.00/mg 2 or more

Clone: FB11

This clone has been derived from hybridization of Sp2/0 myeloma cells with spleen cells of a Balb/c mice immunized with Fr. tularensis.


Specificity: MoAb FB11 recognise LPS of virulent and vaccine strains of Fr. tularensis. The binding site for MoAb FB11 is located on the O-antigen polysaccharide chain which consists of tetrasaccharide fragments and has the following structure:-4)a-D-Galp-NAcAN-(1-4)-a-D-GalpNAcAN-(1-3)-B-D-QuipNac-(1-2)- B-Quip4NFm-(1, Tetrasaccharide D-GalpNAcAN-(1-4)- a-D-GalpNAcAN-(1-3)-B-D-QuipNAc-(1-2)-B-D-Quip4NFm and trisaccharide D-GalpNAcAN-(1-3)-B-D-QuipNAc- (1-2)-B-D-Quip4NFm compete in ELISA for binding MoAb FB11 with LPS of Fr. tularensis.

MoAb FB11 are able to precipitate LPS and do not reveal.In ELISA and the diffusion precipitation reaction, any polysaccharides obtained from LPS by hydrolysis with 1% CH3COOH or by treatment with 0.1 N NaOH.

Reactivity: In immunofluorescence studies MoAb FB11 do not display any cross-reactivity with Fr.novicida.Br. abortus, Br.suis, Br.melitensis, Br.ovis,Y.pestis. Y. enterocolitica, Y. pseudotuberculosis, E.Coli, V. cholerae. Titre of FB11 antibodies not less than 1:10 000 in indirect ELISA using purified LPS Fr.tularensis as a coating antigen.

Recommended dilution: 1:10000 in ELISA/indirect immunofluorescence (phosphate buffer). As second antibody we recommend to use anti-mouse IgG.

Mouse isotype: IgG2a

Application: For diagnosis of tularemia: recognition of tularemia agent and its LPS-containing antigens.

Purification: Purified from ascites by treatment with caprilic acid and by gel-filtration using TSK-TUOPEARL HW 55F. Store +4C.

Packaging: appro x1mg/ml in PNBS with 0.1% NaN3


FOR IN VITRO RESEARCH USE ONLY


RDI Divison of Fitzgerald Industries Intl

34 Junction Square Drive

Concord MA 01742-3049

USA

phone (978) 371-6446 or (800) 370-2222

fax     (978) 371-2266

EMAIL:antibodies@fitzgerald-fii.com

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