rev: 5/15/2008
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Adeno-Associated Virus ANTIBODIES
(anti-Human and others as indicated)
RDI Division of Fitzgerald Industries Intl offers a wide line of antibodies. Since no one antibody works best for all applications (neutralization, blotting, ELISA, etc), we offer many different types of antibodies to help solve this problem. Please inquire for other applications or types of antibodies not listed below.
ANTI-Adeno-Asscociated Virus , VP1 & VP2 & VP3
Mab to VP 1, VP 2 and VP 3 of AAV (adeno-associated virus)
cat#RDI-PRO61058, new cat#: 10R-A114a $525.00/50 µg
Clone
B1
Category Mouse monoclonal
Form/Purification Protein A purified from culture supernatant
Ig Class IgG1
Immunogen Adeno-associated virus particles
Clinical Application/
Specificity Useful for immunolocalization studies of capsid formation in combination with monoclonals A20 (cat#RDI-PRO61055, new cat#: 10R-A110a) and rabbit polylconal antibody to AAV capsid proteins (cat#RDI-PRO61084, new cat#: 20R-AR002). B1 reacts with VP 1, VP 2 and VP 3 of adeno-associated virus and trace amountsof assembled capsids. VP1 and VP2 are highly enriched in the nucleus, while non-assembled VP3 is evenly distributed in the nucleus and the cytoplasm. The antibody is useful for characterization of different stages of infection. Epitope mapping experiments identified aa726 to aa733 (C terminus;common to all 3 VP proteins) as the specific binding region of clone B1
Application · ELISA
· Immunofluorescence
· Immunohistochemistry (also after fixation with formaldehyde)
· Immunoprecipitation (mainly free VP proteins)
· Immunoblotting
· Affinity chromatography
Antigen Recognized
in Species (tested so far) AAV-2 Human, monkey and all published serotypes (AAV-4 only weak cross reaction)
Dilution Buffer PBS or Tris with 0.5% BSA and 0.1% NaN3
Incubation Time 1 h at room temperature for immunohistochemical application
Storage/Stability At 4°C for min. 1 year, after reconstitution with 1 ml dist. water
Quantity , 50 µg (lyoph.) contains 0.5% BSA and 0.09% NaN3.
Literature
Ruffing M, Zentgraf H, JA Kleinschmidt (1992). Assembly of Viruslike Particles by Recombinant Structural Proteins of Adeno-Associated Virus Type 2 in Insect Cells. J Virol. Vol 66, No 12:6922-6930
Wistuba A. Weger S., Kern A. and Kleinschmidt J. (1995) Intermediates of adeno-associated virus type 2 assembly:Identification of soluble complexes containing Rep and Cap proteins. J. Virol. 69, 5311-5319.
Wistuba A. Weger S., Kern A. and Kleinschmidt J. (1997) Subcellular compartmentalization of adeno-associated virus type 2 assembly. J. Virol. 71, 1341-1352.
Jpn J Infec Dis 52 (1999)
J Virology 74, 9281-9293 (2000)
J Virology 76, 12023-12031 (2002)
J Virology 76, 2043-2055 (2002)
For research use only. Not supplied for use in human diagnostic or therapeutic
procedures
RDI Div of Fitzgerald Industries Intl
(Research Diagnostics Inc)
phone (800) 370-2222
or (978) 371-6446 or (800) 370-2222
fax (978) 371-2266
email: sales@researchd.com
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