rev:       June 9, 2005

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Anti- Virus  ANTIBODIES  

(anti-Human and others as indicated)

RDI Divison of Fitzgerald Industries Intl  offers a wide line of  antibodies. Since no one antibody works best for all applications (neutralization, blotting, ELISA, etc), we offer many different types of antibodies to help solve this problem. Please inquire for other applications or types of antibodies not listed below.


Mab to human Papillomavirus  (see 8 clones below)
DATA SHEET: Mouse anti-Human Papillomavirus TYPE 18 monoclonal

Background: Infection with specific types of human papillomavirus (HPV) has been associated with an increased risk of developing cervical neoplsia. HPV types 6 and 11 have been associated with relatively benign diseases as genital warts but types 16 and 18 are strongly associated with cervical, vaginal and vulvar malignancies.

Catalog#: RDI-HPV18-5A3

Price: $469.00/vial

Batch:

Package Size: 2ml lyophilized (tissue culture supernatant containing 15mM sodium azide). Reconstitute with 1ml distilled water.

Clone: 5A3

Isotype: mouse IgG1

Activity: Useful for detection of replicating HPV type 18 and is specific for the L1 coat protein

Application -frozen sections 1:10 dilution

                   -paraffin section when using high temp release

                   -western blotting (not recommended, not optimal)

Storage: Store at 4 Deg C or below. Store reconstituted material in aliquots at -20 DEG C. Avoid frequent freeze thaw cycles.

Precautions:For In vitro research Use Only. Not for usein or on humans or animals or for diagnostic It is the responsibility of the user to comply with all local/state and Federal rules in the use of this product. We are not responsible for any patent infringements that might result with the use of or derivation of this product.


DATA SHEET:   Mouse anti-Human Papillomavirus TYPE (6,11,18)   monoclonal

Background: Infection with specific types of human papillomavirus (HPV) has been associated with an increased risk of developing cervical neoplsia. HPV types 6 and 11 have been associated with relatively benign diseases as genital warts but types 16 and 18 are strongly associated with cervical, vaginal and vulvar malignancies.

Catalog#: RDI-HPVX-4C4

Price: $469.00/vial

Batch:

Package Size: 2ml lyophilized (tissue culture supernatant containing 15mM sodium azide). Reconstitute with 1ml distilled water.

Clone: 4C4

Isotype: mouse IgG1

Activity: Useful for detection of replicating HPV type 6,11 and 18 in cervical biopsies.

Application -frozen sections 1:5 dilution

                     -paraffin sectiosn using high temp release

                     -western blotting

Storage: Store at 4 Deg C or below. Store reconstituted material in aliquots at -20 DEG C. Avoid frequent freeze thaw cycles.

Precautions:For In vitro research Use Only. Not for use in or on humans or animals or for diagnostic It is the responsibility of the user to comply with all local/state and Federal rules in the use of this product. We are not responsible for any patent infringements that might result with the use of or derivation of this product.


Monoclonal mouse anti-E7 protein of human Papillomavirus type 16 (HPV)

Catalogue # RDI-TRK3HP1-281     (for clone#716-281)    

                    RDI-TRK3HP1-325     (for clone#716-325)

$406.00/mg   $375.00/mg 10+  Bulk quotes on request

Clones: 716-281, 716-325   These clones have been derived from hybridization of SP2/0 myeloma cells with spleen cells of Balb/c mice immunised with clones 716-281; 716-332

Mouse isotype: 716-281-IgG2b,  716-325-IgG2a

Application: Mab 716-281 is recommended to be used as a capture antibody and 716-325 as a conjugate in sandwich ELISA. Both clones are suitable for Western Blotting. Both antibodies are cross-reacting with E7 protein of HPV type 18.

Purification: Purified by chromatography on protein G Sepharose. Purity is tested by electrophoresis.

Presentation: Each vial contains antibodies in PBS, pH 7,2, containing 0,1% of sodium azide as preservative. Store at - 20 °C.

Material safety note: This product is sold as an antibody preparation for research purpose only. Standard Laboratory Practices should be followed when handling this material. Contains sodium azide (0,1%) as preservative. Although the amount of sodium azide is very small appropriate care must be taken when handling this product.

For Research Use Only


MOUSE MONOCLONAL  ANTIBODY TO: HPV-16 LATE 1 PROTEIN

PRODUCT CODE  RDI-CBL402    $375.00/vial

CLONE          Cam Vir-1

ISOTYPE       IgG2a

SOURCE       Tissue culture supernatant

PURIFICATION METHOD    Protein A affinity chromatography

SPECIFICITY      This antibody recognizes late protein of human papilloma virus type 16. HPV-6 & -II positive biopsies gave negative results. In western blotting CamVir-1 detects a 41 kDa protein that is observed in a wide range of uninfected mammalian cells, this unwanted specificity is absent by immunoprecipitation, immunofluorescence or immunoperoxidase. This may be a reflection of cross-reactivity between HPV-16 L1 and a cryptic epitope in mammalian cells that is only revealed following exhaustive denaturation.


APPLICATIONS

*Detection of HPV in cervical smears and biopsies

*CamVir-1 recognises HPV-16 LI by immunoprecipitation, western blotting, immunofluorescence on cells fixed in acetone or paraformaldehyde and immunoperoxidase staining of paraffin sections

*Application to diagnostic kits

PRESENTATION   The monoclonal is presented at a concentration of 200µg/2ml in phosphate buffered saline containing 10mM sodium azide and 1mg/ml bovine serum albumin. We recommend that each laboratory determine an optimum working titre for use in its particular application.

STORAGE For use within 1 month of purchase store at +4oC, for long term storage aliquot antibody into small volumes and store at -20oC.

Bulk quotes upon request


MOUSE MONOCLONAL ANTIBODY TO: HUMAN PAPILLOMA VIRUS EARLY PROTEIN

PRODUCT CODE    RDI-CBL403    $375.00/vial

CLONE       BF 7

ISOTYPE     IgG1

SOURCE     Tissue culture supernatant

PURIFICATION METHOD   Protein A affinity chromatography

SPECIFICITY    This antibody reacts with human papilloma virus early protein E6.

APPLICATIONS

*Histology and diagnostic kits for the detection of HPV in cervical smears and biopsy specimens

LOT SPECIFIC DATA

PRESENTATION   The monoclonal is presented at a concentration of 200µg/2ml in phosphate buffered saline containing 10mM sodium azide and 1mg/ml bovine serum albumin. We recommend that each laboratory determine an optimum working titre for use in its particular application.

STORAGE For use within 1 month of purchase store at +4oC, for long term storage aliquot antibody into small volumes and store at -20oC.

For Research Use Only


Monoclonal mouse anti-E7 protein of human Papillomavirus type 18 (HPV)

Catalogue #

RDI-TRK3HP18-15      (clone 718-15)

RDI-TRK3HP18-67      (clone 718-67)

RDI-TRK3HP18-238      (clone 718-238)

$406.00/1mg   $375.00/mg 10+

Clones: 718-15, 718-67, 718-238

Mouse isotype: IgG1 - 718-15, IgG2a - 718-67, IgG2b - 718-238.

Application: The antibodies are working in ELISA and Western Blotting.

Recommended pairs for sandwich ELISA:718-15 or 718-67 for coating and 718-238 as conjugate.

Mabs do not cross-react with E7 protein of HPV type 16.

Purification: Purified by chromatography on protein G Sepharose. Purity is tested by electrophoresis.

Presentation: Each vial contains antibodies in PBS, pH 7.2, containing 0.1% of sodium azide as preservative. Store at + 4 °C.

Material safety note: This product is sold as an antibody preparation for research purpose only. Standard Laboratory Practices should be followed when handling this material. Contains sodium azide (0.1%) as preservative. Although the amount of sodium azide is very small appropriate care must be taken when handling this product.

NOTE: Recommend centrifuging the unopened vial at 500-1000 RPM for 2 minutes to concentrate antibody in bottom of vial. If desired, you can rinse into a 1ml sterile vial with PBS containing 0.1% NaN3 to bring volume to 1mg in 1ml.

For Research Use Only-not for use in or on humans or animals-not for use in diagnostics


RDI div of Fitzgerald Industries Intl

34 Junction Square Drive

Concord MA 01742-3049  USA

phone (978) 371-6446

fax     (978) 371-2266

email: antibodies@fitzgerald-fii.com

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