rev: May 14, 2008
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Anti- Virus ANTIBODIES
(anti-Human and others as indicated)
RDI Division of Fitzgerald Industries Intl offers a wide line of antibodies. Since no one antibody works best for all applications (neutralization, blotting, ELISA, etc), we offer many different types of antibodies to help solve this problem. Please inquire for other applications or types of antibodies not listed below.
Mab to HIV-1 p24 (see 4 clones & 1 polyclonal below)
see also pure viral protein
Monoclonal mouse anti-HIV p24
CAT#: RDI-TRK3H24-ND1, new cat#: 10R-H120c
PRICE: $412.50/1mg
Clone: ND1 This clone has been derived from hybridization of Sp2/0 myeloma cells with spleen cells of a Balb/c mice immunised with p24 C terminal peptide.
Specificity: HIV-1 p24 by immunoblot/Elisa
Mouse isotype: IgG1
Application: Detection of P24 in Elisa and western blot
Purification: Purified by DEAEcchromatography Purity is tested by electrophoresis.
Presentation: Each vial contains 1 mg of antibodies with con- centration mg/ml in 0.ml,in PBS, pH 7.4, containing 0.1% of sodium azide as preservative.
Material safety note: This product is sold as an antibody preparation for research purpose only. Standarad Laboratory Practices should be followed when handling this material. Contains sodiium azide (.01%) as pre- servative. Although the amount of sodium azide is very small appropriate care must be taken when handling this product.
FOR IN VITRO RESEARCH USE ONLY
NOTE:
Recommend centrifuging the unopened vial at 500-1000 RPM for 2 minutes to concentrate antibody in bottom of vial. If desired, you can add ul PBS containing 0.1% NaN3 to bring volume to 1mg in 1ml
DATA SHEET: Mouse Monoclonal anti-HIV-I GP24
Catalog#: RDI-HIVP24abm-26 - Discontinued
Price: $500.00 plus shipping $438.00/vial 2 or more
Package Size: 100ug/vial
Supplied: lyophilized: Reconstitute with 0.5ml sterile DI H20 to yield a
200ug/ml stock in PBS pH 7.2. Let set at least 30 minutes at 4-8 DEG C , Mix
well. If desired, centrifuge for 1-2 minutes at 1000 rpm to concentrate
antibody in vial.
Ig Isotype: IgG2b
USE: Binds to HIV-1 p24 in Western blot and Elisa. approx epitope
is on peptide 25 (aa 154-172)
Minimum dilution: 1:200 (for blotting, overnight 4 DEG C with chemiluminescence detection)
Storage: Store at 4-8 DEG C. After reconstitution, recommend aliquoting and store in working aliquots at -20 DEg C or below. Avoid frequent freeze thaw cycles.
Precautions:For In vitro research Use Only. Not for
use in or on humans oranimals or for diagnostics. It
is the responsibility of the user to comply with all local/state and Federal
rules in the use of this product. We are not responsible for any patent
infringements that might result with the use of or derivation of this product.
see also antibodies to: gp120, gp41, tat, rev, nef, RT, integrase and more.. available purified, FITC, Biotin and Hrp conj
Goat anti-HIV P24 (aff pure) Polyclonal
cat# RDI-HIVP24abGX, new cat#: 20R-HG004
Price: $209.00/1mg
Presentation: IgG fraction in PBS buffer, pH 7.2 with 0.1% NaN3.
Immunogen: Highly pure HIV-1 p24
Host: goat
Titer: approx 1:100,000 tests/ml by EIA ; 5.0 mg/ml
Cross Reactivity: Specific for p24 ; No reactivty to human T or B cells.
Storage: 4°C or -20°C for long term storage
Remarks: Tested by EIA and Western blot
For Research or Futher Manufacturing Use Only
anti-HIV-1 p24 antibody (monoconal)
CAT#: RDI-HIVP24abm, new cat#: 10R-H120a $412.50/1mg
Description: HIV-1 p24 IgG (monoclonal). Produced
against a recombinant protein.
Specificity: HIV-1 p24 antigen.
Origin: Mouse
Clone: 1A1
Purity: Affinity purified
Buffer: 1X PBS pH 7.2;0.01% sodium azide.
Concentration:-1 mg/ml;bulk amount available upon
request.
Recommended Dilution: For ELISA: 1:1000. For Western Blot: 1:1000.
Storage: Short term: 4'C. Long term: -20'C. Avoid frequent freeze thaw cycles
For In vitro research Use Only. Not for use in or on humans or animals or for diagnostics.It is the responsibility of the user to comply with all local/state and Federal rules in the use of this product. We are not responsible for any patent infringements that might result with the use or derivation of this product.
Note: Can be custom
FITC conjugated cat#RDI-HIVP24abm-FTX $1250.00/1 milligram $1125.00/mg 2 or more
or biotin labeled cat#RDI-HIVP24abm-BTX $1250.00/1 milligram $1125.00/mg 2 or more
Mouse anti-HIVp24 capsid antigen (Paraffin Sections)
cat# RDI-HIVP24abm-K1, new cat#: 10R-H133a $525.00/vial
Specificity: Human immunodeficiency virus (HIV) type 1 capsid
protein p24 and its precursor,p55. No reaction is observed with HIV type 2 or
simian immunodeficiency virus
Clone: Kal-1
Ig Class: IgG1k
Antigen: purified human immunodeficiency virus type 1 disrupted with 0.5% NP40
Hybridoma partner: Mouse myeloma (p3-X63-Ag8.563).
Preparation: Lyophilised tissue culture supernatant
containing 15mM sodium azide. Reconstitute with 1ml
of sterile distilled water.
USE: Effective on paraffin wax embedded tissue using 1mM EDTA pH 8.0 unmasking
solution combined with the high temperature antigen unmasking technique -not
tested for frozen sections (see other clones)
Protocol: Immunohistochemistry:Typical working
dilution 1:10 - 1:25. High temperature antigen unmasking technique using 1mM
EDTA (pH 8.0). 60 Minutes incubation at 25'C. Standard ABC technique.
Positive controls: HIV-positive lymph node
Staining pattern: granular staining in close proximity to the membrane of
infected cells -reacts with lymphocytes, monocytes,
macrophages, langerhans cells of the skin, follicular
dendritic cells and brain cells of microglia lineage
infected with HIV type 1.
Storage and stability: Store unopened lyophilized antibody at 4'C. Under these
conditions, there is no significant loss in product performance up to the
expiry date indicated on the vial label. The reconstituted antibody is stable
for at least two months when stored at 4'C. For long term storage, aliquot in
non frost free freezer, Avoid repeated freeze thaw cycles. Prepare fresh
working dilutions daily.
Refs: -Path Res Pract 188:91-96(1992)
-American Journal f Pathology, 137(2):247-251 (1990)
Stricly for in vitro research use only-not for use in diagnostics
Sample High Temperature Unmasking Technqiue for Paraffin Sections:
Buffer=1mM EDTA (ph 8.0) or 0.01M sodium Citrate Buffer (pH 6.0)
1) Cut and mount sections on slides coated with "apes"
2) Deparrafinize sections and rehydrate to distilled water
3) Bring 1600ml 1mM EDTA* (pH 8.0) to a boil in a Prestige/Presto stainless
steel pressure cooker, using a hot plate. Cover but do not lock lid. *or
0.01M sodium citrate buffer (pH 6.0)-not for CD4 ab
4) Position slides into metal staining racks and lower into pressure cooker
ensuring slides are well immersed in buffer Lock the lid. The small valve will
rise.
5) When the pressure indicator valve (the large one) has risen after about
4minutes, Incubate sections for 1 minute.
6) After timer rings, Remove pressure cooker from heat source and run under
cold water with lid on. When the small valve sinks open lid and remove slides
and place immediately into distilled water. DO NOT OPEN LID UNTIL THE SMALL
VALVE Sinks or Vent Cover Lock Drops (see manual with your particular unit).
7) Wash sections PBS buffer (10mM phosphate, 0.15M NaCl
pH 7.5) for 2 X 5 minutes (or 100mM TRIS, 0.15M NaCl,
pH 7.5)
8) Place sections in 1.5% hydrogen peroxide/methanol for 10 minutes (or use
other appropriate peroxidase blocking procedure).
9) Wash sections in distilled water for 2 X 5 minutes, then wash sections in
PBS buffer for 2 X 5 minutes.
10) Place sections in normal serum for 20 minutes.
|11) Cover sections with primary antibody for at least 60 minutes at room temp.
(Optimum dilution, incubation time and temperature must be determined for each
antibody and in each lab).
12)
13) Incubate sections in secondary antibody for 30 minutes
14) Wash in PBS buffer for 2 X 5 minutes
15) Incubate slides in ABC complex for 30 minutes
16)
17) Incubate slides in DAB or other suitable peroxidase
substrate
18)
19) Counterstain with haematoxylin
(if required), dehydrate, coverslip, and mount
Material:
APES (3-aminopropyltriethoxysilane) cat#A3648 Sigma
To avoid sections becoming detached, sections should be mounted on "APES" covered slides, then dried at 37 DEG C overnight followed by drying at 56 DEG C for 60 minutes.
1mM EDTA (ph 8.0): Add 0.37g of EDTA (Sigma cat#E-5134) to 1 liter of distilled water. Adjust pH to 8.0 using 1.0M sodium hydroxide.
Tris: 100mmM Tris, 0.15M NaCl, pH 7.5.
PBS: 10mM Phosphate, 0.15M NaCl pH 7.5
RDI Division of Fitzgerald Industries Intl
phone (978) 371-6446 or (800) 370-2222
fax (978) 371-2266
EMAIL:antibodies@fitzgerald-fii.com
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