rev:  April 25, 2002

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Anti- Virus  ANTIBODIES  

(anti-Human and others as indicated)

RDI Divison of Fitzgerald Industries Intl  offers a wide line of  antibodies. Since no one antibody works best for all applications (neutralization, blotting, ELISA, etc), we offer many different types of antibodies to help solve this problem. Please inquire for other applications or types of antibodies not listed below.


Giardia Intestinalis (see 2 clones below)
Mouse anti-Giardia intestinalis (monoclonal)   Histochemsitry

cat# RDI-GIARDabm-9D   $469.00/vial

Presentation: 1ml lyophilised tissue culture supernatant containing 15mM sodium azide. Reconstitute with 1ml sterile distilled water, et set at leats 30 minutes. Mix.

Clone 9D5.3.1

Isotype: mIgG2b

Immunogen: Freeze-thawed,sonicated trophozoites from in vitro culture.

Hybridoma  partner: Mouse myeloma (JK Ag8.653).

Specificity: Giardia intestinalis (Portland 1 strain)(formerly Giardia lamblia ).External surface of trophozoite including flagellae.

Use: formalin fixed parrafin sections with high temp citrate buffer antigen release.

Dilutions: Recommendations on use Immunohistochemistry:

                 Typical working dilution 1:25 -1:50.

                High temperature antigen unmasking technique.

                60 minutes primary antibody incubation at 25 o C.

               Standard ABC technique.

Pos ctrl: Giardia intestinalis infected small intestine.

Staining: pattern staining of protozoa on the surface of luminal epithelia.

Storage: Store unopened lyophilised antibody at 4 o C.Under these conditions,there is no significant loss in product performance up to the expiry date indicated on the vial label.The reconstituted antibody is stable for at least two months when stored at 4 o C.For long term storage,it is recommended that aliquots of the antibody are frozen at -20 o C (frost-free freezers are not recommended).Repeated freezing and thawing must be avoided.Prepare working dilutions on the day of use.

FOR RESEARCH USE ONLY .

Backgorund:

Giardia intestinalis (formerly Giardia lamblia )is a flagellated protozoan which infects man via contaminated water supplies,causing illnesses ranging from acute severe bloody diarrhoea,through moderate enteritis,chronic diarrhoea with malabsorption,to asymptomatic excretion.The remarkable hardiness of the cyst form and the low numbers required to infect make the epidemiology uncertain,although water-based infections are the most common. This Mab is suitable for immunohistochemical detection of Giardia intestinalis in tissue, including the jejunal biopsy specimen often used to diagnose the chronic disease with malabsorption.

References

Ortega Y R and Adam R D.Giardia:Overview and update.Clinical Infectious Diseases.25 :545-550 (1997).

Baruch A C,Isaac-Renton J and Adam R D.The molecular epidemiology of Giardia lamblia :A sequence-based approach.The Journal of Infectious Diseases.174 :233-236 (1996).

Rosenthal P and Liebman W M.Comparative study of stool examinations,duodenal aspiration,and pediatric Entero-Test for giardiasis in children.The Journal of Pediatrics.96(2):278-279 (1980).

Kamath K R and Murugasu R.A comparative study of four methods for detecting Giardia lamblia in children with diarrheal disease and malabsorption.Gastroenterology.66(1):16-21 (1974).


Mouse anti-Giardia intestinalis (monoclonal)   Immunofluorescence

cat# RDI-GIARDabm-22  $469.00

also available: FITC conjugated: cat#RDI-GIARDabm-22FT   $500.00/100T

Presentation: 1ml lyophilised tissue culture supernatant containing 15mM sodium azide. Reconstitute with 1ml sterile distilled water, et set at leats 30 minutes. Mix.

Clone: 22A6

Isotype: mIgG1

Immunogen: Whole formalin-fixed Giardia cysts.

Specificity Giardia intestinalis (formerly Giardia lamblia ).

Use; Not for use on paraffin sections Recommendations on use Indirect immunofluorescence: Typical working dilution 1:10 -1:20. Read using immersion oil eg Cargille type FF .The use of a fluorescence enhancing mounting medium is not recommended.

Pos ctrl: Giardia cysts.

Staining: pattern Cyst-like.

Storage: Store unopened lyophilised antibody at 4 o C.Under these conditions,there is no significant loss in product performance up to the expiry date indicated on the vial label.The reconstituted antibody is stable for at least two months when stored at 4 DEG C.For long term storage,it is recommended that aliquots of the antibody are frozen at -20 DEG C (frost-free freezers are not recommended).Repeated freezing and thawing must be avoided.Prepare working dilutions on the day of   use.

FOR RESEARCH USE ONLY .

Background:

Giardia intestinalis (formerly Giardia lamblia )is a flagellated protozoan,which infects man via contaminated water supplies,causing illnesses ranging from acute severe bloody diarrhoea,through moderate enteritis,chronic diarrhoea with malabsorbtion,to asymptomatic excretion.The remarkable hardiness of the cyst form and the low numbers required to infect make the epidemiology uncertain,although water-based infections are the commonest. This Mab is suitable for the detection of both in vivo and in vitro -grown Giardia intestinalis cysts by indirect immunoflurescence.

References

Ortega Y R and Adam R D.Giardia:Overview and update.Clinical Infectious Diseases.25 :545-550 (1997).

Baruch A C,Isaac-Renton J and Adam R D.The molecular epidemiology of Giardia lamblia :A sequence-based approach.The Journal of Infectious Diseases.174 :233-236 (1996).

Ward H D,Kane A V,Ortega-Barria E,et al..Identification of developmentally regulated Giardia lamblia cyst antigens using GCSA-1,a cyst-specific monoclonal antibody.Molecular Microbiology.4 (12):2095-2102 (1990).

Rosenthal P and Liebman W M.Comparative study of stool examinations,duodenal aspiration,and pediatric Entero-Test for giardiasis in children.The Journal of Pediatrics.96(2):278-279 (1980).

Kamath K R and Murugasu R.A comparative study of four methods for detecting Giardia lamblia in children with diarrheal disease and malabsorption.Gastroenterology.66(1):16-21 (1974).

For In vitro research Use Only. Not for use in or on humans or animals or for diagnostics.It is the responsibility of the user to comply with all local/state and Federal rules in the use of this product. We are not responsible for any patent infringements that might result with the use or derivation of this product.


RDI Divison of Fitzgerald Industries Intl

34 Junction Square Drive

Concord MA 01742-3049

USA

phone (978) 371-6446 or (800) 370-2222

fax     (978) 371-2266

EMAIL:antibodies@fitzgerald-fii.com

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