rev: March 1, 2000

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Adeno-Virus  ANTIBODIES  

(anti-Human and others as indicated)

RDI Divison of Fitzgerald Industries Intl  offers a wide line of  antibodies. Since no one antibody works best for all applications (neutralization, blotting, ELISA, etc), we offer many different types of antibodies to help solve this problem. Please inquire for other applications or types of antibodies not listed below.

ANTI-Adeno Virus
Mouse anti-Adenovirus Cocktail   (clones M58 + M73)

cat# RDI-ADENOVCKabm       $406.00/100ug

       RDI-ADENOVCKabm-1    $562.00/200ug

Presentation: Protein A purified antibody in 10mM PBS with 0.2% BSA and 15mM NaN3. (Bulk available without BSA/Azide upon request) concentration 200ug/ml

Reactivity: adenovirus

Clone: cocktail of clones M58 + M73

Isotype: mIgG2ak

Immunogen: adenovirus

Hybridoma: Produced by fusion between BALB/c splenocytes and mouse myeloma NS-1 cells.

Uses: -immunofluorescence: 1:500 60 min RT

           -western blot 1:200 with chemiluminescence  detection

          -histochemsitry: formalin fixed paraffin sections using ABC/Dab detection

          -immunoprecipitation: 10ul/mg protein lysate

Positive Ctrl: human renal Ca Cells-2, 293 cells or adenovirus infected tissues

Localization: nuclear

Background: The early region (E1A) of the adenovirus genome plays a central role in cellular transformation and regulation of gene expression. The adenovirus early gene product E1A is a potent stimulator of cellular proliferation, which when overexpressed can overcome the growth-inhibitory effects of TGF-beta. The E1A region encodes a series of related proteins (35-46kDa) with multifuctional capabilities and form a specific complex with the retinoblastoma tumor suppressor gene product (Rb protein). The E1A and E1B regions together comprise the transforming region of adenovirus. While expression of E1A alone is sufficient to immortalize primary cells, complete transformation requires the additional expression of the E1B region. Several conserved regions of E1A are similar to portions of other viral oncoproteins like the HPV-16 and HPV-18 E7 and SV40 large T antigen. This Mab immunoprecipitates E1A associated proteins.

Storage: 4-8 DEG C

Individual clones available on request


For research use only. Not supplied for use in human diagnostic or therapeutic procedures

RDI Divison of Fitzgerald Industries Intl

34 Junction Square Drive

Concord MA 01742-3049

USA

phone (978) 371-6446 or (800) 370-2222

fax     (978) 371-2266

EMAIL:antibodies@fitzgerald-fii.com

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