rev: 5/16/2008
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Adeno-Virus
ANTIBODIES
(anti-Human and others as indicated)
RDI Divison of Fitzgerald Industries Intl offers a wide line of antibodies. Since no one antibody works best for all applications (neutralization, blotting, ELISA, etc), we offer many different types of antibodies to help solve this problem. Please inquire for other applications or types of antibodies not listed below.
ANTI-Adeno Virus
Mouse anti-Adenovirus Fiber Protein (monomer & trimer)
cat# RDI-ADENOV2abm $484.00/100ug
RDI-ADENOV2abm-1 $719.00/200ug
RDI-ADENOV2abm-1X $781.00/200ug no azide
Presentation: Protein A purified antibody in 10mM PBS with 0.2% BSA and 15mM
NaN3. (Bulk available without BSA/Azide upon request) concentration 200ug/ml
Reactivity: adenovirus
Clone: 4D2
Isotype: mIgG2ak
Immunogen: Uv-irradiated Ad2 virus
Hybridoma: Produced by fusion between BALB/c splenocytes and mouse myeloma NS-1
cells.
Uses: -immunofluorescence: 1:50 60 min RT
-western blot 1:100-1:200 with
chemiluminescence detection
Positive Ctrl: adenovirus infected cells and tissues
Localization: nuclear
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Background: It recognizes proteins of 62kDa and 180-200kDa which are identified
as the monomer and trimer of adenovirus 2 (Ad2), Adenovirus 5 (Ad5), and
Adenovirus 7 (Ad7) fiber protein. Adenovirus capsid proteins are synthesized in
the cytoplasm and transported to the nucleus for assembly into the virus
particles. The three major capsid proteins (hexon, penton base, and fiber) are
synthesized late in infection. Fiber plays a crucial role in adenovirus
infection by attaching the virus to a specific receptor on the cell surface.
Ad2 and Ad5 fibers are proposed to consist of three domains: an N-terminal tail
that interacts with penton base, a shaft composed of 22 repeats of a 15 amino
acid segment that forms beta-sheet and beta-bends, and a knob at the C-terminus
that contains the type-specific antigen and is responsible for binding to the
cell surface receptor. It is shown that the fiber of Ad2 is most likely a
trimer when found on the viron . This Mab recognizes
both monomers and trimers, as judged by reactivity with both boiled (monomers
(62kDa) only) and unboiled (both monomers and trimers (180- 200kDa bands))
fiber on Western blots and by indirect immunofluorescence studies of cells
infected with a temperature-sensitive Ad5 fiber mutant (H5ts142) which does not
form trimers at the nonpermissive temperature at both 32 DEG C and 39.5 DEG C
(1)
Storage: 4-8 DEG C
references: 1. Hong, J.S., Engler, J.A. The amino terminus of the adenovirus
fiber protein encodes the nuclear localization signal. Virology.,
185, 758-767,1991.
2. Hong, J.S., Engler, J.A. Domains required for assembly of adenovirus type 2 fiber trimers. J. Virology, 70, 7071-7078, 1996.
For research use only. Not supplied for use in human
diagnostic or therapeutic procedures
RDI Divison of Fitzgerald Industries Intl
phone (978) 371-6446 or (800) 370-2222
fax (978) 371-2266
EMAIL:antibodies@fitzgerald-fii.com
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