rev: June 11, 2004
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ANTIBODIES
(anti-Human and others as indicated)
RDI Divison of Fitzgerald Industries Intl offers a wide line of antibodies. Since no one antibody works best for all applications (neutralization, blotting, histochemistry, ELISA, etc), we offer many different types of antibodies to help solve this problem. Please inquire for other applications or types of antibodies not listed below.
Catalog#: RDI-PAPabm-4LJ $406.00/200ug in 1ml w/BSA/azide
RDI-PAPabm-4LBT $562.00/200ug Biotin conjug
RDI-PAPabm-4LXP $469.00/200ug/0/2ml no azide
Presentation: Protein G purified antibody in 10mM PBS with 0.2% BSA and 0.09% NaN3. (Bulk available without BSA/Azide upon request) concentration 200ug/ml
Immunogen: purified prostatic acid phosphatase (PSAP) from human seminal plasma
Isotype: mIgG1
Clone: PASE/4LJ
Hybridoma: fusion between Swiss A2G splenocytes and mouse myeloma P3XAAg8.653 (NS1) cells
Reactivity: human, dog, rabbit and rat (others not tested)
Uses: -Western blot: (reported)
-histochemistry (frozen and paraffin sections) approx 1ug/ml for 60 minutes RT
Positive Control: normal prostate or prostate carcinoma
Localization: Cytoplasmic
ref: Haines AM et al. British Journal of Cancer 1989 DEC, 60(6):887-92
Background: Recognizes a protein of 52kda, identified as prostate specific acid phosphatase (PSAP). Clone PASE/4LJ is highly specific to PSAP and shows no cross reaction with other phosphatases. It does not inhibit the enzymatic activity of PSAP. This MAB reacts with non- neoplastic adult and fetal prostatic glands, oprimary and metastatic prostatic carcinomas. It shows no staining in granulocytes, osteoclasts, parietal cells of the stomach, liver cells, renal cell or breast carcinomas.
Storage: 2-8 deg c
For Research Use Only
see also rabbit anti-PASAP
cat#RDI-PRO10816 $375.00/vial
Clone
Pase/4LJ
Category
Mouse
monoclonal,purified IgG
Ig Class
IgG1
Immunogen
Isolated from human seminal plasma
Clinical Application/Specificity This mab, reactive with prostatic-specific
acid phosphatase, recognizes benign and malignant prostatic epithelium. In
some cases pancreatic islet cells, carcinomas of the bladder, and carcinoids
may also be stained. The antibody reacts with a 52 kDa protein.
Recommended for:
Positive Control Prostatic benign hyperplasia
Application ELISA
Immunoblotting
Immunohistochemistry Suitable for frozen and paraffin* sections
* no pretreatment required
Antigen Recognized in species
(tested so far) Human
Working Dilution
1:10 - 1:20 Dilution Buffer PBS with 1%
BSA and 0.1% Na-Azide
Incubation Time
1 h at room temperature
Storage At
4°C (undiluted)
or at -20°C (aliquots)
Volume 1 ml
References
Swanholm H. et al. Evaluation of commercial immunoperoxidase kits for prostatic specific antigen and prostatic specific acid phosphatase (1986). Acta Path Microbiol Immunol Scand Sect A, 94, 7-12
For In Vitro Research Use Only-Not For Use in Diagnostics
cat# RDI-PAPabm-X X=clone#
Clone# 501, 502, 503, 504
Lot# see batch sheet
Use: to be determined
Class: mouse IgG1 (clone 502=IgG2a)
Antigen: human seminal plasma
Presentation: Chromatographically purified immunoglobulin fraction in 0.015M
PBS, pH 7.2 containing 0.1% NaN3.
Volume: see batch sheet
Protein conc: see batch sheet
Storage: Store at 4-8 DEG C Upon Receipt. Avoid Frequent freeze thaw
cycles.
For IN VITRO RESEARCH OR FURTHER MANUFACTURING USE ONLY.
Price: $375.00/mg 1-5mg
Test pack: 1 mg each clone $1250.00 ord cat#RDI-PAPabm-PK
For In Vitro or further manufacturing use only
RDI Divison of Fitzgerald Industries Intl
34 Junction Square Drive
Concord MA 01742-3049
USA
phone (978) 371-6446 or (800) 370-2222
fax (978) 371-2266
EMAIL:antibodies@fitzgerald-fii.com
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