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ANTIBODIES  

(anti-Human and others as indicated)

RDI Divison of Fitzgerald Industries Intl  offers a wide line of  antibodies. Since no one antibody works best for all applications (neutralization, blotting, histochemistry, ELISA, etc), we offer many different types of antibodies to help solve this problem. Please inquire for other applications or types of antibodies not listed below.


DATA SHEET: Mouse anti-human PAP/PSAP   (Prostatic Acid Phosphatase) PURIFIED FORMAT

Catalog#: RDI-PAPabm-4LJ          $406.00/200ug in 1ml w/BSA/azide

                RDI-PAPabm-4LBT      $562.00/200ug Biotin conjug

                 RDI-PAPabm-4LXP     $469.00/200ug/0/2ml no azide

Presentation: Protein G purified antibody in 10mM PBS with 0.2% BSA and 0.09% NaN3. (Bulk available without BSA/Azide upon request) concentration 200ug/ml

Immunogen: purified prostatic acid phosphatase (PSAP) from human seminal plasma

Isotype: mIgG1

Clone: PASE/4LJ

Hybridoma: fusion between Swiss A2G splenocytes and mouse myeloma P3XAAg8.653 (NS1) cells

Reactivity: human, dog, rabbit and rat (others not tested)

Uses: -Western blot: (reported)

-histochemistry (frozen and paraffin sections) approx 1ug/ml for 60 minutes RT

Positive Control: normal prostate or prostate carcinoma

Localization: Cytoplasmic

ref: Haines AM et al. British Journal of Cancer 1989 DEC, 60(6):887-92

Background: Recognizes a protein of 52kda, identified as prostate specific acid phosphatase (PSAP). Clone PASE/4LJ is highly specific to PSAP and shows no cross reaction with other phosphatases. It does not inhibit the enzymatic activity of PSAP. This MAB reacts with non- neoplastic adult and fetal prostatic glands, oprimary and metastatic prostatic carcinomas. It shows no staining in granulocytes, osteoclasts, parietal cells of the stomach, liver cells, renal cell or breast carcinomas.

Storage: 2-8 deg c

For Research Use Only

see also rabbit anti-PASAP


Mab to Prostatic Acid  Phosphatase (PAP)

cat#RDI-PRO10816   $375.00/vial


Clone                                               Pase/4LJ

Category                                         Mouse monoclonal,purified IgG

Ig Class                                           IgG1

Immunogen                                      Isolated from human seminal plasma

Clinical Application/Specificity This mab, reactive with prostatic-specific acid phosphatase, recognizes benign and malignant prostatic epithelium. In some cases pancreatic islet cells, carcinomas of the bladder, and carcinoids may also be stained. The antibody reacts with a 52 kDa protein.

Recommended for:

Positive Control                             Prostatic benign hyperplasia

                                                     

Application                                    ELISA

                                                      Immunoblotting

                                                      Immunohistochemistry  Suitable for frozen and paraffin* sections  

                                                                                    * no pretreatment required

Antigen Recognized in species

(tested so far)                                 Human


Working Dilution                          1:10 - 1:20 Dilution Buffer PBS with 1% BSA and 0.1% Na-Azide

Incubation Time                            1 h at room temperature

Storage At                                   4°C (undiluted) or at -20°C (aliquots)

Volume                                         1 ml


References

Swanholm H. et al. Evaluation of commercial immunoperoxidase kits for prostatic specific antigen and prostatic specific acid phosphatase (1986). Acta Path Microbiol Immunol Scand Sect A, 94, 7-12

      For In Vitro Research Use Only-Not For Use in Diagnostics


Mouse anti-Human PAP MONOCLONAL

cat# RDI-PAPabm-X     X=clone#

Clone# 501, 502, 503, 504

Lot# see batch sheet

Use: to be determined

Class: mouse IgG1 (clone 502=IgG2a)

Antigen: human seminal plasma

Presentation: Chromatographically purified immunoglobulin fraction in 0.015M PBS, pH 7.2 containing 0.1% NaN3.

Volume: see batch sheet

Protein conc: see batch sheet

Storage: Store at 4-8 DEG C Upon Receipt. Avoid Frequent freeze thaw cycles.

For IN VITRO RESEARCH OR FURTHER MANUFACTURING USE ONLY.

Price: $375.00/mg 1-5mg  

Test pack: 1 mg each clone $1250.00 ord    cat#RDI-PAPabm-PK

For In Vitro or further manufacturing use only


RDI Divison of Fitzgerald Industries Intl

34 Junction Square Drive

Concord MA 01742-3049

USA

phone (978) 371-6446 or (800) 370-2222

fax     (978) 371-2266

EMAIL:antibodies@fitzgerald-fii.com

Ordering terms

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