rev: January 11, 2005
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ANTIBODIES
(anti-Human and others as indicated)
RDI Divison of Fitzgerald Industries Intl offers a wide line of antibodies. Since no one antibody works best for all applications (neutralization, blotting, histochemistry, ELISA, etc), we offer many different types of antibodies to help solve this problem. Please inquire for other applications or types of antibodies not listed below.
Rabbit (monoclonal) anti-NEU/Her-2/c-erb2 Human
cat# RDI-NEUabrM $438.00/0.5ml
RDI-NEUabrM1 $625.00/1ml
clone# SP3
form: culture supernatant with 0.09% sodium azide
immunogen: Recombinant protein encoding extracellular domain of human c-erbB2
species: human (others not tested)
use: formalin fixed paraffin sections 1:100
[Staining of formalin-fixed tissues requires boiling tissue sections in 10mM citrate buffer, pH 6.0 for 10-20 min followed by cooling at RT for 20 min.] The optimal dilution for a specific application should be determined by the investigator.
staining pattern: cell membrane
positive control: breast carcinoma tissue or SKBR3 cells
Storage: Store 2-8 DEG C
Background: c-erbB-2 is a receptor tyrosine kinase of the c-erbB family. It is closely related instructure to the epidermal growth factor receptor. c-erbB-2 oncoprotein is detectable in a proportion of breast and other adenocarconomas, as well as transitional cell carcinomas. In the case of breast cancer, expression determined by immunohistochemistry has been shown to be associated with poor prognosis.
For In Vitro Research Use Only
cat#RDI-NEUabrX $438.00/0.5mg $625.00/1mg
form: 1mg/ml in PBS with 0.2% BSA and 0.09% sodium azide
-also available BSA/Azide free cat#RDI-NEUabrXP $688/1mg
-immunogen:a synthetic peptide from C terminus of human C-erB2 (identical to corresponding rat sequence)
species: human, mouse , rat and monkey (others not tested)
-use: western blot (185kda), immunoprecipitation, histochemistry parrafin sections
-staining pattern: cell membrane
-positive control:breast carcinoma tissue or SKBR3 cells
-blocking peptide for histo: cat#RDI-NEUabRX-CP $156.00/200ug with 0.2% BSA and 0.09% NaN3
PRODUCT CODE RDI-CBL772 $375.00/vial
CLONE 300G9
ISOTYPE IgG1
IMMUNIZING ANTIGEN NIH 3T3 transfectant
FUSION PARTNER NS1 myeloma cell line
SOURCE Mouse ascitic fluid
PURIFICATION METHOD Protein A affinity chromatography
SPECIFICITY
The antibody recognizes the truncated version of HER 2, HER-2 ECD which has a wide tissue distribution closely similar to that of EGFR. The epitope recognized is a perinuclear cytoplasmic protein.
APPLICATIONS
*Western blotting
*Immunoprecipitation
*Frozen tissue sections
*Formalin fixed paraffin wax embedded tissue sections from patients where HER 2 is over expressed e.g. breast, stomach, ovarian and bladder carcinoma
LOT SPECIFIC DATA
PRESENTATION
The monoclonal is presented at a concentration of 200µg/2ml in phosphate
buffered saline containing 10mM sodium azide and 1mg/ml bovine serum albumin.
We recommend that each laboratory determine an optimum working titre for
use in its particular application.
STORAGE For use within 1 month of purchase store at +4oC, for long term storage
aliquot antibody into small volumes and store at -20oC.
For Research Use Only.
34 Junction Square Drive
Concord MA 01742-3049
USA
phone (978) 371-6446 or (800) 370-2222
fax (978) 371-2266
EMAIL:antibodies@fitzgerald-fii.com
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