rev:      January 11, 2005

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ANTIBODIES  

(anti-Human and others as indicated)

RDI Divison of Fitzgerald Industries Intl  offers a wide line of  antibodies. Since no one antibody works best for all applications (neutralization, blotting, histochemistry, ELISA, etc), we offer many different types of antibodies to help solve this problem. Please inquire for other applications or types of antibodies not listed below.


RE: Neu Oncogene Antibodies
Neu (HER2) Antibodies

Rabbit (monoclonal) anti-NEU/Her-2/c-erb2 Human

cat# RDI-NEUabrM     $438.00/0.5ml

       RDI-NEUabrM1    $625.00/1ml

clone# SP3

form: culture supernatant with 0.09% sodium azide

immunogen: Recombinant protein encoding extracellular domain of human c-erbB2

species: human (others not tested)

use: formalin fixed paraffin sections 1:100

[Staining of formalin-fixed tissues requires boiling tissue sections in 10mM citrate buffer, pH 6.0 for 10-20 min followed by cooling at RT for 20 min.]   The optimal dilution for a specific application should be determined by the investigator.

staining pattern: cell membrane

positive control: breast carcinoma tissue or SKBR3 cells

Storage: Store 2-8 DEG C

Background: c-erbB-2 is a receptor tyrosine kinase of the c-erbB family. It is closely related instructure to the epidermal growth factor receptor. c-erbB-2 oncoprotein is detectable in a proportion of breast and other adenocarconomas, as well as transitional cell carcinomas. In the case of breast cancer, expression determined by immunohistochemistry has been shown to be associated with poor prognosis.

For In Vitro Research Use Only


Rabbit (Polyclonal) anti-NEU/Her-2/c-erb2 Human/Mouse/Rat/Monkey

cat#RDI-NEUabrX   $438.00/0.5mg      $625.00/1mg

form: 1mg/ml in PBS with 0.2% BSA and 0.09% sodium azide

-also available BSA/Azide free cat#RDI-NEUabrXP  $688/1mg

-immunogen:a synthetic peptide from C terminus of human C-erB2 (identical to corresponding rat sequence)

species: human, mouse , rat and monkey (others not tested)

-use: western blot (185kda), immunoprecipitation, histochemistry parrafin sections

-staining pattern: cell membrane

-positive control:breast carcinoma tissue or SKBR3 cells

-blocking peptide for histo: cat#RDI-NEUabRX-CP  $156.00/200ug with 0.2% BSA and 0.09% NaN3


inquire for other monoclonals (clones 9G6.10, L87, N12, N24, N28, N29, e2-4001, B10, CB11, 3B5, L26, Pn2A, 2ERB19 -inquire with your application
MOUSE MONOCLONAL ANTIBODY TO: HER-2 gp185 (HER-2 ECD)

PRODUCT CODE  RDI-CBL772      $375.00/vial

CLONE        300G9

ISOTYPE      IgG1

IMMUNIZING ANTIGEN    NIH 3T3 transfectant

FUSION PARTNER NS1 myeloma cell line

SOURCE   Mouse ascitic fluid

PURIFICATION METHOD    Protein A affinity chromatography

SPECIFICITY

The antibody recognizes the truncated version of HER 2, HER-2 ECD which has a wide tissue distribution closely similar to that of EGFR. The epitope recognized is a perinuclear cytoplasmic protein.

APPLICATIONS

*Western blotting

*Immunoprecipitation

*Frozen tissue sections

*Formalin fixed paraffin wax embedded tissue sections from patients where HER 2 is over expressed e.g. breast, stomach, ovarian and bladder carcinoma

LOT SPECIFIC DATA

PRESENTATION

The monoclonal is presented at a concentration of 200µg/2ml in phosphate buffered saline containing 10mM sodium azide and 1mg/ml bovine serum albumin. We recommend that each laboratory determine an optimum working titre for use in its particular application.

STORAGE For use within 1 month of purchase store at +4oC, for long term storage aliquot antibody into small volumes and store at -20oC.

For Research Use Only.


RDI Divison of Fitzgerald Industries Intl

34 Junction Square Drive

Concord MA 01742-3049

USA

phone (978) 371-6446 or (800) 370-2222

fax     (978) 371-2266

EMAIL:antibodies@fitzgerald-fii.com

Ordering terms

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