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ANTIBODIES  

(anti-Human and others as indicated)

RDI Divison of Fitzgerald Industries Intl  offers a wide line of  antibodies. Since no one antibody works best for all applications (neutralization, blotting, histochemistry, ELISA, etc), we offer many different types of antibodies to help solve this problem. Please inquire for other applications or types of antibodies not listed below.


RE: Mucin (Mucin-1, Mucin-2, Mucin-3, Mucin-5) & related Antibodies

-see also muc-6 glycoprotein


Monoclonal mouse anti-human MUC1 glycoprotein

Catalogue # RDI-TRK4M40-xxxx (xxx=insert clone number per below)

Price: $438.00/1mg $406.00/mg 2-10 $375.00/mg 11+

Clone: CM1, X19

             New clones - M9E7, M4H2, M8C9, M10G4, M10H6

             New clones - M4H2, M2F1, M2C5, M2E104, M3A106

These clones have been derived from hybridization of X63-Ag8-653 myeloma cells with spleen cells of Balb/c miceimmunized with high molecular weight (more than 300 kDa) glycoprotein from human milk-fat globule molecule.

Specificity: Clone CM1 - Peptide tandem repeat in MUC1 core.

                                Similar to DF3 clone (Centocor).Affinity more 10 9 M -1  

                    Clone X19 - peptide repeat RPAP region

                    Clones M9E7, M4H2, M8C9, M10G4, M10H6: Affinity > 1 x 10 8 for all clones. Mabs bind with high efficiency with different regions of GVTSAPDTRPAPGSTAPPAHGVTSA synthetic peptide spanning the one repeat of VNTR extracellular portion of MUC1 molecule. They react with VNTR5 and VNTR20 recombinant unglycosylated fragments of MUC1 protein and underglycosilated MUC1 prepared from tumor fluids or as a result of chemical treatment of human milk MUC1. These Mabs do not recognize natural MUC1 protein isolated from human milk by affinity purification on carbohydrate epitope specific Mabs and belong to Mab cluster 1-4 according to ISOMB TD-4 classification . Non crossreactive with egg white avidin.

Clones M4H2, M2F1, M2C5, M2E10 and M2A10: A ffinity > 1 X 10 8 for all clones. Mabs bind with high efficiency with different epitopes within the VNTR tandem repeat peptide region of MUC1 molecule, which have different conformations and  peptide- carbohydrate compositions. Mab M4H2 react with VNTR20  recombinant unglycosylated fragment of MUC1 protein (but not with monomeric MUC1 peptide), with deglycosilated and underglycosylated natural MUC1 (but not with completely glycosylated MUC1). Mabs M2F1, M2C5, M2E104, M3A106 efficiently recognize underglycosylated and natural MUC1 protein isolated from human milk by affinity chromatography. All these Mabs belong to Mab clusters 6 and 7 according to ISOMB TD-4 classification. Non crossreactive with normal serum proteins and human tissues of nonepithelial origin.

For research Use Only


#RDI-CBL204         mouse anti-human milk mucin core antigen SM3

$375.00/200ug         (see ref: Canc. Res. 47, 5476-82 (1987) ).


#RDI-CBL406     (Note:Product cat#RDI-CBL406, has been discontinued.)     mouse anti-colonic & pancreatic mucin

$375.00/200ug         (probably designated CA50) clone Cam 17.1


#RDI-CBL205         mouse anti-Lewis A antigen clone PR5C5

$375.00/200ug        (reacts with lewis A antigen, colonic mucus, epithelial cells and cell membranes).


#RDI-PanMuc-B12  mouse anti-human pan-mucin producing cell

$500.00/100Tests     clone b-12 useful for identifying mucin-like carcinoma antigens (MCA) produced by various tumors and certain healthy glandular cells. In combination with other BMA markers for inflammation staging or investigating neo vascularization processes, clone b-12 is a valuable tool for studying tumor growth/regression. mIgG1k, suitable for frozen and paraffin sections.


RDI-MUC1abm      mouse anti-human mucin-1 glycoprotein MUC-1 Ma695

$438.00/1ml           (see ref: Human Pathology 25, 364-372 (1994).


see also epithelial membrane antigen

(EMA/CA15-3/MUC1) antibodies BULK ON REQUEST


RDI-MUC1abm-E39   mouse anti-MUC1(CXA153) EMA clone E39

$438.00/200ug          clone E39 recommended for formalin fixed paraffin sections (no pretratemnet needed)


RDI-MUC1abm-4H5    mouse anti-epithelial membrane antigen (MUC1)

$375.00/200ug              clone VU-4H5. suitable for ELISA, IP, WB, histochemistry (frozen ,and formalin fixed/paraffin sections with high temp release). A pan-epithelial marker for detecting early metastatic loci of carcinoma in the bone marrow or liver.m IgG1 Inquire for Bulk -


RDI-MUC1abm-GP       mouse anti-Mucin-1 clone GP1.4  mIgG1  (western blotting and histochemistry, paraffin sections)   $438.00/200ug
 RDI-MUC1abm-E29        mouse anti-Mucin-1 clone E29 , mIgG2a (histochemitry paraffin sections, human only)

$375.00/200ug    


RDI-MUC1CORabm     mouse anti-human mucin-1 core glycoprotein Ma552

$438.00/1ml                  (see ref: Human Pathology 25, 364-372 (1994).


RDI-MUC2abm          mouse anti-mucin-2 glycoprotein MUC-2 clone Ccp58

$438.00/1ml               (useful in colonic and gastric tumours to detect MUC-2 also known as intestinal-Mucin Related Protein) Inquire for Bulk purified with/wo BSA.


RDI-MUC3abm         mouse anti-MUCIN 3 (MUC3) clone M3.1

$375.00/100ug          -useful in ELISA, western blotting, histochemistry

$625.00/200ug          -reacts with both colon carcinoma as well as normal colon. Muc3 is distributed in colon and rectum, and is also present to a lesser extent in breast, lung and salivary glands. (ref:Journal of Gastroenterology and Hepatology, 1995,10:555-561 Inquire for BULK purified with/without BSA.


RDI-MUC5ACabm     mouse anti-MUCIN 5AC/Gastric Mucin clone 45M1

$375.00/100ug          -useful in western blotting (non reducing),

$625.00/200ug           ethanol fixed cells, histochemistry (forzen and formalim/paraffin reacts with the peptide of gastric mucin M1 (>1,000 kDa). Reacts with gastric epithelium of normal gastrointestinal tract as well as with the precancerous colon but not with adult colon. It also reacts with fetal colonic mucosa. human, rat, pig, chicken, mouse, monkey, rabbit and feline reactive (not with bovine) ref:International journal of Cancer, 1991 47 (2);304-10.


RDI-MUCGASabm      mouse anti-human gastric mucin clone 45M1

$438.00/1ml                reacts with surface epithelium of the normal gastrointestinal tract, but not with normal colon. The antibody also reacts with fetal colonic mucosa, adenomas, hyperplastic polyps, carcinomas and mucosa adjacent to carcinomas.


RDI-PSAabmX          mouse anti-human PSA (Prostate Specific Antigen)

$375.00/vial              (suitable for parrafin embedded tissues) 1ml lyophilized

See also antibodies again human milk fat globule HMFG1 and HMFG2


Monoclonal anti-Mucin 1 Core Glycoprotein

cat# RDI-MUC1CORabm  $438.00/vial

Specificity:*       recognizes a hexapeptide (TRPAPG) in the tandem   repeat region of the Muc-1 core glycoprotein.

Presentation:     1 ml lyophilized supernatant with 15mM sodium azide added.

Clone:               Ma552

Isotype:             mIgG1

Antigen:             human breast cell line ZR75-1

Hybridoma:       mouse myeloma (Sp 2/0)

USES:             -frozen section

                        -Paraffin sections (using high temp unmasking technique)

                        -typical dilutions:1:50-1:100, 30 minute   incubation at 25 DEG C.

Stain:                Cytoplasmic and extracellular membrane   associated staining pattern.

Controls:           Breast carcinoma (a high percentage will stain positive)

Reconstitution:   1 ml distilled water.

Storage:             Store lyophilized material at 4 DEG C. Store reconstituted material at 4 DEG C for

                         immediate use. For long term use, recommend aliquoting and store at -20 DEG C. Avoid

                         frequent freeze thaw cycles.


*NOTE: Although this antibody was made against ZR75-1 cancer cells and is regarded as a breast associated cancer antigen, it also reacts with a proportion of adenocarcinomas from other sites. The clinical and biological significance of expression of the MUC-1 core antigen has not yet been established.


FOR IN VITRO RESEARCH USE ONLY-NOT FOR USE IN DIAGNOSTICS


Monoclonal anti -Mucin 1 Glycoprotein

cat# RDI-MUC1abm     $438.00/vial

Specificity:*                  recognizes a carbohydrate epitope of the Muc-1 glycoprotein.

Presentation:                1 ml lyophilized supernatant with 15mM sodium azide added.

Clone:                          Ma695

Isotype:                       mIgG1

Antigen:                      human breast cell line ZR75-1

Hybridoma:               mouse myeloma (P3X63-Ag8.653)

USES:                     -frozen section

                               -Paraffin sections (using high temp unmasking technique)

                               -typical dilutions:1:100, 30 minute incubation at 25 DEG C.

Stain:                        Cytoplasmic and extracellular membrane associated staining pattern.

Controls:                  Breast carcinoma (a high percentage will stain positive)

Reconstitution:         1 ml distilled water.

Storage:                   Store lyophilized material at 4 DEG C.

                               Store reconstituted material at 4 DEG C for immediate use. For long term use, recommend

                              aliquoting and store at -20 DEG C. Avoid   frequent freeze thaw cycles.


*NOTE: Although this antibody was made against ZR75-1 cancer cells and is regarded as a breast associated cancer antigen, it also reacts with a proportion of adenocarcinomas from other sites. The clinical and biological significance of expression of the MUC-1 antigen has not yet been established.


FOR IN VITRO RESEARCH USE ONLY-NOT FOR USE IN DIAGNOSTICS


Mouse anti-MUC 1 (CA15-3, Epithelial Membrane Antigen)   Paraffin sections

cat# RDI-MUC1abm-E39       $438.00/200ug

       RDI-MUC1abm-E39X    $500.00/200ug (NO BSA/NO Azide)

Presentation: Protein G purified antibody in 10mM PBS with 0.2% BSA and 15mM NaN3. (Bulk available without BSA/Azide upon request) concentration 200ug/ml

Immunogen: human milk fat globule membranes

Isotype: mIgG1k

Clone: E39

Reactivity: human (others not tested)

Uses: -histochemistry (frozen sections and formalin fixed) approx 5-2ug/ml 60 minutes RT no pretreatment necessary

Positive Ctrl: breast or colon carcinoma

Localization: Cytoplasmic and cell membrane

Background: Epithelial membrane antigen (EMA), or CA15-3, or polymorphic epithelial mucin (PEM), or sialomucin, or episialin is a mucin-like glycoprotein. Antibody to EMA can be useful as a pan- epithelial marker for detecting early metastatic loci of carcinoma in the bone marrow or liver. MW of antigen approx 265-400Kda.

Storage: 2-8 DEG C

For Research Use Only


Mouse anti-MUC 1 (CA15-3, Epithelial Membrane Antigen)

cat# RDI-MUC1abm-4H5    $438.00/200ug

Presentation: Protein G purified antibody in 10mM PBS with 0.2% BSA and 15mM NaN3. (Bulk available without BSA/Azide upon request) concentration 200ug/ml

Immunogen: BSA conjugate of a pentamer of a synthetic hexapeptide (APPAHG) of the MUC-1 tandem repeat

Isotype: mIgG1k

Clone: VU-4H5

Reactivity: human (others not tested)

Uses: -Western blot: 1:200

           -histochemistry (frozen sections and formalin fixed) approx 1:50-1:100 60 minutes RT (formalin fixed sections work better using high temp release in 10mM citrate buffer,pH6.0, boil for 10-20 minutes with cooloing to room temp for 20 minutes.

           -Elisa: (order BSA free, cat#RDI-MIC1abm-4H5X)

           -immunoprecipiation: 10ul/mg protein

Positive Ctrl: breast or colon carcinoma

Localization: Cytoplasmic and cell membrane

Background: Recognizes a high MW (300-450kda) mucin like glycoprotein identified as epithelial membrane antigen (EMA) or CA 15-3, or sialomucin or episalin. Antibody to EMA has been shown to be useful as a pan-epithelial marker for detecting early metastatic loci of carcinoma in the bonemarrow or liver.

Storage: 2-8 DEG C

For Research Use Only


Mouse anti-MUC 1 (CA15-3, Epithelial Membrane Antigen)  clone GP1.4

cat# RDI-MUC1abm-GP   $438.00/vial 200ug

Presentation: purified antibody (200ug/ml) in PBS with 0.2% BSA and 15mM NaN3

Immunogen: Human milk fat globule membranes

Isotype: mIgG1k

Clone: GP1.4

Reactivity: human (others not tested)

Uses: -Western blot: 1:200

          -histochemistry (frozen sections and formalin fixed) approx 1:50-1:100 60 minutes RT

Positive Ctrl: breast or colon carcinoma

Localization: Cytoplasmic and cell membrane

Background: In western blotting, recognizes two glycoproteins in MW range of 265-400Kda, identified as epithelial membrane antigen (EMA) or mucin 1 (MUC-1) or sialomucin or episalin. Clone GP1.4 reacts with the DTRP epitope in the tandem repeats. Antibody to EMA has been shown to be useful as a pan-epithelial marker for detecting early metastatic loci of carcinoma in the bonemarrow or liver.

Storage: 2-8 DEG C

For Research Use Only


Hamster anti-MUC 1 (human/Mouse)   Paraffin sections, IP, WB

cat# RDI-MUC1abm-MH1      $438.00/100ug

       RDI-MUC1abm-MH1X   $500.00/100ug (NO BSA/NO Azide)

Presentation: 100ug in 0.5ml (200ug/ml) Protein G purified antibody in 10mM PBS with 0.2% BSA and 15mM NaN3. MH1X=100ug in 0.1ml (1mg/ml ) PBS, no BSA/No Azide

Immunogen: A synthetic peptide corresponding to aa 239-255 (SSLSYTNPAVAATSANL) form the cytoplasmic tail of MUC1.

Isotype: ARMENIAN HAMSTER IgG (use anti-armenian hamster specific secondary antibody)

Clone: MH1 (also known as CT2)

Reactivity: human and mouse (others not tested)

Uses: -histochemistry (frozen sections and formalin fixed) approx 5-10ug/ml 60 minutes RT no pretreatment necessary

        -flow cytometry

       -immunofluorescence

       -western blotting

      -immunoprecipitation

reference: Schroeder J A, et al. (2001) J Biol Chem 276:13057-13064.

Positive Ctrl: MCF-7 cells or breast carcinoma

Localization: Cytoplasmic and cell membrane

Background: Muc1 is a heavily O-glycosylated transmembrane protein expressed on most secretory epithelium, including mammary gland and some hematopoietic cells. It is expressed abundantly in lactating mammary glands and overexpressed in >90% breast carcinomas and metastases. In normal mammary gland it is expressed in apical surface of glandular epithelium. In breast cancer Muc1 is overexpressed; is underglycosylated and the apical localization is lost. Muc1 is transcribed as a larger precursor which is cleaved to form a larger mucin like subunit (265-400kDa) and a smaller subunit (14- 28kDa) noncovalently associated with each other. Transgenic Muc1 has been shown to associate with all four erbB receptors and localize with erbB1 (EGFR) in lactating glands 1 . Muc1 can act as a ligand for ICAM-1 on HUVEC cells; it can bind beta-catenin, GSK3beta and it associates with Grb2-SOS upon phosphorylation..

Storage: 2-8 DEG C

For Research Use Only


Monoclonal anti -Mucin 2 Glycoprotein

cat# RDI-MUC2abm      $438.00/vial

Specificity:*                   human muc-2 glycoprotein. Non reactive with muc-1 or muc-3 derived peptides.

Presentation:                1 ml lyophilized supernatant with 15mM sodium azide added.

Clone:                        Ccp58

Isotype:                      mIgG1

Antigen:                     synthetic peptide corresponding to a site on the muc-2 glycoprotein

Hybridoma:              mouse myeloma (p3-NS1-Ag4-1)

USES:                    -frozen section

                              -Paraffin sections (using high temp unmasking technique)

                              -typical dilutions:1:100-1:200, 30 minute incubation at 25 DEG C.

Stain:                     Cytoplasmic and extracellular membrane associated staining pattern.

Controls:               small bowel

Reconstitution:      1 ml distilled water.

Storage:                Store lyophilized material at 4 DEG C.

                             Store reconstituted material at 4 DEG C for   immediate use (uo to 2 months). For long term

                             use, recommend aliquoting and store at -20 DEG   C in non frost free freezer. Avoid

                             frequent freeze thaw cycles.


Background: MUc-2 glycoprotein also known as intestinal mucin related protein antigen is an intestinal apomucin.Muc-2 glycoprotein is expressed in mucinous tumors but not in serous tumors. Expression of MUC-2 glycoprotein is found in a low percentage of borderline malignancies and in approx 55 percent of carcinomas where Muc-1 glycoprotein is also expressed. muc-2 is useful for the detection of MUC-2 glycoprotein in colonic and gastric tumors.

ref: Xing, P-X et al, J. Natl. Cancer Inst. 84:699- 703 (1992)


FOR IN VITRO RESEARCH USE ONLY-NOT FOR USE IN DIAGNOSTICS


Mouse anti-MUC 3

cat# RDI-MUC3abm     $375.00/100ug

       RDI-MUC3abm-1   $625.00/200ug

Presentation: Protein G purified antibody in 10mM PBS with 0.2% BSA and 15mM NaN3. (Bulk available without BSA/Azide upon request) concentration 200ug/ml

Immunogen: synthetic peptide of 35 amino acids, SIB35, which contains two of the MUC3 tandem repeats coupled to KLH     -C-H S T P S F T S S I T T T E T T S H S T P S F T S S I T T T E T T S

Isotype: mIgG2a

Clone: M3.1

Reactivity: human (others not tested)

Uses: -Western blot: 1ug/ml

         -histochemistry (frozen sections)

         approx 1:50-1:100 60 minutes RT

        -Elisa: (order BSA free)

Positive Control: colon carcinoma and normal colon tissue

Localization: Cytoplasmic and extracellular membrane

Background: Recognizes a protein of high molecular weight identified as mucin 3 (MUC3) glycoprotein. Its epitope localizes between aa SITTE. Shows no cross reaction with human milk fat globule membranes, MUC1 or MUC2. Has been reported to react with both colon carcinoma as well as normal colon tissue. Muc3 is distributed in colon and rectum, and is also present to a lesser extent in breast, lung and salivary gland tissues.

Storage: 2-8 DEG C

reference: Apostolopoulos V, et al. Journal of Gastroenterology and Hepatology, 1995, 10:555-61

For Research Use Only


Mouse anit-mucin 3 (paraffin sections)

cat#RDI-MUC3abmn-B7   $469.00/100ug

cat#RID-MUC3abm-B7X  $812.00/200ug

-clone ref:1143/B7

-human reactive (not tested with other species)


Mouse anti-MUC 5AC

cat# RDI-MUC5ACabm          $375.00/100ug in 0.5ml

        RDI-MUC5ACabm-1       $625.00/200ug

        RDI-MUC5ACabm-BT   $500.00/100ug Biotin conjugated

        RDI-MUC5ACabm-BT   $750.00/200ug Biotin conjugated

Presentation: Protein G purified antibody in 10mM PBS with 0.2% BSA and 15mM NaN3. (Bulk available without BSA/Azide upon request) concentration 200ug/ml

Immunogen: M1 mucin preparation from the fluid of an ovarian mucinous cyst belonging to an O Le(a-b-) patient

Isotype: mIgG1

Clone: 45M1

Epitope: destroyed by B-mercaptoethanol and proteases but not by periodate treatement (Cancer Res, 1886, 3983)


Reactivity: human, monkey, rabbit, cat, mouse, rat, pig, hedgehog, and chicken (does not react with cow). (others not tested)

Uses: -Western blot(non reducing):1-2ug/ml 2-3 HR RT or overnight 4 DEG C with chemiluminescence detection -histochemistry (frozen and formalin fixed paraffin sections, no special pretreatment) approx 1-2ug/ml 60 minutes RT

-cell staining (ethanol fixed)

Positive Ctrl: stomach

Localization: Cytoplasmic and cell surface


Background: Recognizes the peptide core of gastric mucin M1 (>1,000kDa), recently identified as mucin 5AC). Its epitope is destroyed by B-mercaptoethanol and proteases but not by periodate treatment. Antibody to gastric mucin M1 reacts with the gastric epithelium of normal gastrointestinal tract as well as with the precancerous and cancerous colon but not with normal adult colon. Its also reacts with fetal colonic mucosa. Resurgence of gastric mucin reactivity during colonic carcinogenesis is due to reexpression of the peptide core of gastric (or fetal colonic) mucin.|

Storage: 2-8 DEG C

reference: -Bara et al. International Journal of Cancer, 1991, 47(2):304-10

                 -Bara et al. Journal of Immunological Methods, 1992, 149(1):105-13

                -Cancer Res 1886, 46:3983-3989 

For Research Use Only

see also clone 1-13M1 cat#RDI-MUC5AC-M1   $375.100ug   or   cat#RDI-MUC5AC-M1X $625.00/200ug


RDI Divison of Fitzgerald Industries Intl

34 Junction Square Drive

Concord MA 01742-3049

USA

phone (978) 371-6446 or (800) 370-2222

fax     (978) 371-2266

EMAIL:antibodies@fitzgerald-fii.com

Ordering terms

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