rev: January 25, 2005
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ANTIBODIES
(anti-Human and others as indicated)
RDI Divison of researchd Industries Intl offers a wide line of antibodies. Since no one antibody works best for all applications (neutralization, blotting, ELISA, etc), we offer many different types of antibodies to help solve this problem. Please inquire for other applications or types of antibodies not listed below.
cat# RDI-MUC6Aabm-H5 $500.00/1ml
Presentation: Lyophilized tissue culture supernatant containing 15 mM sodium
azide. Reconstitute with 1 ml sterile distilled water . Let set at least
30 minutes, mix well.
Clone: CLH5
Hybridoma partner: Mouse myeloma (p3-NS1-Ag4-1).
Isotype: mIgG1
Immunogen: synthetic peptide of the MUC-6 tandem repeat sequence purified
by HPLC.
Specificity: Human Muc-6 glycoprotein
Use: Effective on paraffin wax embedded tissue using citrate buffer unmasking
solution combined with high temperature antigen unmasking techniques.
Recommendations on use: Immunohistochemistry: Typical working dilution
1:50-1:100. 60 minute primary antibody incubation at 25 DEG C. Standard ABC
technique.
-not tested on frozen sections
-not tested for Western blotting:
Positive controls: stomach
Staining pattern: gastric glands, cytoplasmic
Storage: Store unopened lyophilized antibody at 4'C. Under these conditions,
there is no significant loss in product performance up to the expiry date
indicated on the vial label. The reconstituted antibody is stable for at
least two months when stored at 4'C. For long term storage, it is recommended
that aliquots of the antibody are frozen at -20'C (frost-free freezers are
not recommended). Repeated freezing and thawing must be avoided. Prepare
working dilutions on the day of use.
For Research Use Only
Application:
Mucins are heavily glycosylated glycoproteins which constitute the major components of mucus which cover the surface of epithelial tissues. Nine distinct epithelial mucin genes MUC-1, 2,3,4, 5AC, 5B, 6, 7 and 8) have been identified. Various immunohistochemical and in situ hybridization stidues have shown that these mucins are differentially expressed in epithelia with cell-type specificity. The normal gastric mucosa shows cell-type specific expression of MUC-1, MUC-5AC and MUC-6 glycoproteins with the first two mucins found in superficial epithelium and MUC6 glycoprotein in the deep glands. Muc-1 and MUC-5AC glycoproteins are expressed in many epithelia but MUC-6 glycoprotein is mainly expressed in gastric mucosa. In addition, Muc-2 glycoprtoein is not expressed in normal gastric mucosa. Changes in the expression levels and glycosylation patterns of mucins have been associated with several diseases. In gastric cancer, alterations in mucin polypeptide expression have been reported, including th eloss of expression of MUC 5-AC glycoprotein, increased mucin heterogeneity glycosylation changes and the expression of simple mucin-type carbohydrates. This Mab may be of use in the detection of MUC-6 glycoprotein where low levels of expression may be an indicator of intestinal metaplasia.
References:
Reis CA et al, Intestinal metaplasia of human stomach displays distinct patterns
of mucin (MUC1, MUC2, MUC5AC and MUC6) expression Cancer Res. 59(5):1003-1007
(1999)
Sasaki M et al, Expression of MUC2, MUC5AC and MUC6 apomucins in carcinoma,
dysplasia and non-dysplastic epithelia of the gallbladder, Pathol Int.
49(1):38-44(1999)
Bartman, A E et al, The MUC6 secretory mucin gene is expressed in a wide
variety of epithelial tissues. J Pathol 186. 186(4):398-405 (1998)
For Research Use Only
RDI Divison of researchd Industries Intl
San Jose, 95123 CA Snell ave 658
USA
or 408-780-0908
EMAIL:margaret@cellular-research.com
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