rev:    January 25, 2005

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  ANTIBODIES  

(anti-Human and others as indicated)

RDI Divison of researchd Industries Intl  offers a wide line of  antibodies. Since no one antibody works best for all applications (neutralization, blotting, ELISA, etc), we offer many different types of antibodies to help solve this problem. Please inquire for other applications or types of antibodies not listed below.

-see also other mucin antibodies
Mouse anti-MUC-6 Glycoprotein

cat# RDI-MUC6Aabm-H5     $500.00/1ml

Presentation: Lyophilized tissue culture supernatant containing 15 mM sodium azide. Reconstitute with 1 ml sterile distilled water . Let set at least 30 minutes, mix well.

Clone: CLH5

Hybridoma partner: Mouse myeloma (p3-NS1-Ag4-1).

Isotype: mIgG1

Immunogen: synthetic peptide of the MUC-6 tandem repeat sequence purified by HPLC.

Specificity: Human Muc-6 glycoprotein

Use: Effective on paraffin wax embedded tissue using citrate buffer unmasking solution combined with high temperature antigen unmasking techniques.


Recommendations on use: Immunohistochemistry: Typical working dilution 1:50-1:100. 60 minute primary antibody incubation at 25 DEG C. Standard ABC technique.

-not tested on frozen sections

-not tested for Western blotting:

Positive controls: stomach

Staining pattern: gastric glands, cytoplasmic

Storage: Store unopened lyophilized antibody at 4'C. Under these conditions, there is no significant loss in product performance up to the expiry date indicated on the vial label. The reconstituted antibody is stable for at least two months when stored at 4'C. For long term storage, it is recommended that aliquots of the antibody are frozen at -20'C (frost-free freezers are not recommended). Repeated freezing and thawing must be avoided. Prepare working dilutions on the day of use.

For Research Use Only

Application:

Mucins are heavily glycosylated glycoproteins which constitute the major components of mucus which cover the surface of epithelial tissues. Nine distinct epithelial mucin genes MUC-1, 2,3,4, 5AC, 5B, 6, 7 and 8) have been identified. Various immunohistochemical and in situ hybridization stidues have shown that these mucins are differentially expressed in epithelia with cell-type specificity. The normal gastric mucosa shows cell-type specific expression of MUC-1, MUC-5AC and MUC-6 glycoproteins with the first two mucins found in superficial epithelium and MUC6 glycoprotein in the deep glands. Muc-1 and MUC-5AC glycoproteins are expressed in many epithelia but MUC-6 glycoprotein is mainly expressed in gastric mucosa. In addition, Muc-2 glycoprtoein is not expressed in normal gastric mucosa. Changes in the expression levels and glycosylation patterns of mucins have been associated with several diseases. In gastric cancer, alterations in mucin polypeptide expression have been reported, including th eloss of expression of MUC 5-AC glycoprotein, increased mucin heterogeneity glycosylation changes and the expression of simple mucin-type carbohydrates. This Mab may be of use in the detection of MUC-6 glycoprotein where low levels of expression may be an indicator of intestinal metaplasia.

References:

Reis CA et al, Intestinal metaplasia of human stomach displays distinct patterns of mucin (MUC1, MUC2, MUC5AC and MUC6) expression Cancer Res. 59(5):1003-1007 (1999)

Sasaki M et al, Expression of MUC2, MUC5AC and MUC6 apomucins in carcinoma, dysplasia and non-dysplastic epithelia of the gallbladder, Pathol Int. 49(1):38-44(1999)

Bartman, A E et al, The MUC6 secretory mucin gene is expressed in a wide variety of epithelial tissues. J Pathol 186. 186(4):398-405 (1998)

For Research Use Only

RDI Divison of researchd Industries Intl

San Jose, 95123 CA Snell ave 658

USA

or 408-780-0908

EMAIL:margaret@cellular-research.com

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