rev:  November 11, 2002

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(anti-Human and others as indicated)

RDI Divison of researchd Industries Intl  offers a wide line of  antibodies. Since no one antibody works best for all applications (neutralization, blotting, histochemistry, ELISA, etc), we offer many different types of antibodies to help solve this problem. Please inquire for other applications or types of antibodies not listed below.

Mouse anti-Involucrin (purified format)

cat# RDI-INVOLUabmx       $375.00/100ug Bulk quotes on request

        RDI-INVOLUMabmX  $500.00/200ug

       RDI-INVOLUabmXP    $562.00/200ug NO BSA/NO AZide

       RDI-INVOLUabmx-BT  $469.00/100ug $688.00/200ug Biotin conj

Presentation: Protein G purified antibody in 10mM PBS with 0.2% BSA and 15mM NaN3. (Bulk available without BSA/Azide upon request) concentration 200ug/ml

Clone : SY5

Ig Isotype: IgG1

Immunogen: Human keratinocytes’ involucrin

Mol. Wt.Antigen: 66-170kDa

Epitope: Codon 421-568

ref: Hudson DL, et. al. Hybridoma, 1992, 11:367.

Applications: ELISA (For coating, order Ab without BSA, cat#RDI-INVOLabmXP)


                        Immunoprecipitation (Denatured verified) (Use Protein G) (Ab 2µg/mg protein lysate)

                       Western Blotting (Ab 1-2µg/ml for 2hrs at RT)

                       Immunohistology (Formalin/paraffin) 1-2ug/ml 30 min room temp

                       [For staining of formalin-fixed tissues, digest sections with trypsin at 1mg/ml PBS, 10 min at 37 o C or protease XXV at 1mg/ml PBS for 5 minutes at 37°C * (Note than enzyme digestion is better than citrate buffer based epitope unmasking)

The optimal dilution for a specific application should be determined by the investigator

Positive Control: LS174T cells. Skin.

Cellular Localization: Cytoplasmic

Description: Involucrin is expressed in a range of stratified squamous epithelia, including the cornea. In normal epidermis, it is first expressed in the upper spinous layers, and in keratinocyte cultures it is expressed by all cells that have left the basal layer. Involucrin expression is abnormal in squamous cell carcinomas and premalignant lesions, and is reduced in severe dysplasias of the larynx and cervix.

Comments: stains the involucrin in a variety of sizes: 170kDa in MCF-7 cells , a doublet of ~115kDa and 150kDa in gorilla and owl monkey , 66kDa in dog , and a doublet of 105kDa in pig .

RDI Divison of researchd Industries Intl

San Jose, 95123 CA Snell ave 658


or 408-780-0908

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