rev: October 14, 2003
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ANTIBODIES
(anti-Human and others as indicated)
RDI Divison of researchd Industries Intl offers a wide line of antibodies. Since no one antibody works best for all applications (neutralization, blotting, histochemistry, ELISA, etc), we offer many different types of antibodies to help solve this problem. Please inquire for other applications or types of antibodies not listed below.
cat# RDI-COLL4abm-COC $406.00/0.5ml
RDI-COLL4abm-COC1 $562.00/1ml
Presentation: Tissue culture supernatant with 0.09% sodium azide,
Isotype: mIgG1
Clone: PHM-12 & CIV 22
Reactivity: human (others not tested)
Uses: Immunohistology (Formalin/paraffin) (Use Ab at 1:50-1:100 for 30 min
at RT) (Staining of formalin/paraffin tissues is enhanced by digestion of
tissue sections with Protease XXV at 1mg/ml PBS for 5 minutes at 37°C
Positive Control: Skin.
Localization: Basement membrane
Background: Collagen IV is a major constituent of the basement membranes along with laminins and enactins. It is composed of á 1(IV) chain and á 2(IV) chain in 2:1 ratio . It can form insoluble fibers with high tensile strength. Antibody to collagen IV is useful in detecting the loss of parts of basement membrane in carcinomas. This MAB cocktail is highly specific to type IV collagen. In kidney, it reacts with glomerular and tubular basement membranes, parts of mesangial matrix and the Bowman’s capsule. It also reacts with basal lamina of capillaries as well as basement membranes in a variety of tissues. This Mab cocktail is excellent for the staining of routine formalin-fixed, paraffin-embedded tissues.
References: 1. Odermatt B F, et. al. 91984) Proc Natl Acad Sci USA, 81:7343-7347.
2. Lee CS; et al. Pathology, 1996, 28(2):135-8.
3. Delaigue O; et al. Graefes Archive for Clinical and Experimental Ophthalmology, 1995 233:
331-8.
Storage: 2-8 deg c
For Research Use Only
-individual abs/clones available on request
Mouse ANTI-COLLAGEN TYPE IV
Mab to Collagen Type IV
cat#RDI-PRO10710 $344.00/vial
Clone 1042
Category Mouse monoclonal, ascites
Ig Class IgG2b
Immunogen Human placental type IV collagen
Clinical Application/Specificity Antibody 1042 is reactive with human collagen
type IV of basement membranes, and shows a homogeneous staining pattern in
all tissues. As neoplastic cells of invasive carcinomas often lack a continuous
basement membrane, the antibody is useful to distinguish between non-invasive
and invasive lesions. Additionally, it can be used for the differentiation
of bullous lesions in dermatopathology. No cross-reactivity with other collagens.
Recommended Positive Control: for Skin, kidney
Application
· ELISA
· Immunoblotting
· Immunohistochemistry
· Suitable for frozen tissue and paraffin* sections; (*proteolytic treatment
required; enzyme: pronase)
Antigen Recognized :Human specific
Working Dilution 1:10 - 1:20
Dilution Buffer PBS with 1% BSA and 0.1% Na-Azide
Incubation Time 1 h at room temperature
Storage At 4°C (undiluted) or at -20°C (aliquots)
Volume 1 ml
References
M.G. Havenitz, J.P.M. Cleutjens, C. Beek, E.v.d.Linden, A.F.P.M. De Goeij, and F.T. Bosman: Human specific anti-type IV collagen monoclonal antibodies, characterization and immunohistochemical applicatiuon. Histochemistry (1987) 87:123-128.
For In Vitro Research Use Only-Not For Use in Diagnostics
cat#RDI-PRO10760 $344.00/vial
Category Rabbit serum
Ig Class IgG
Immunogen Human placental type IV collagen
Clinical Application/Specificity The antibody is reactive with collagen type
IV of basement membranes, and shows a homogeneous staining pattern in all
tissues. As neoplastic cells of invasive carcinomas often lack a continuous
basement membrane, the antibody is useful to distinguish between non-invasive
and invasive lesions. Additionally, it can be used for the differentiation
of bullous lesions in dermatopathology. No cross reactivity with other collagens
at optimal dilutions. In immunoblotting1, a slight cross reactivity with
collagen type V is observed.
Recommended Positive Control for Skin, kidney
Application ELISA
Immunoblotting1
Immunohistochemistry
Suitable for frozen tissue and paraffin* sections; (*proteolytic treatment required; enzyme: pepsin)
Antigen Recognized in species tested so far: Most species incl. human, rat,
mouse, rabbit
Working Dilution 1:50 - 1:100
Dilution Buffer PBS with 1% BSA and 0.1% Na-Azide
Incubation Time 1 h at room temperature or over night at 4°C
Storage At 4°C (undiluted) or at -20°C (aliquots)
Volume 0,25 ml
References
1. F.T. Bosman, M. Havenith, and J.P.M. Cleutjens: Basement Membranes in Cancer. Ustrastructural Pathology (1985) 8:291-304.
2. R. Visser, J.M.H. v.d. Beek, M.G. Havenith, J.P.M. Cleutjens & F.T. Bosman: Immunocytochemical detection of basement membrane antigens in the histopathological evaluation of laryngeal dysplasia and neoplasia.
For In Vitro Research Use Only-Not For Use In Diagnostics
PRODUCT: Affinity Purified Anti-Collagen Type IV (Rabbit) Minimum Cross Reaction to Type I, II, III, V and VI Collagens.
CODE NO.: RDI-600-401-106 $562.00/vial
LOT NO:
PHYSICAL STATE: Liquid (Sterile Filtered)
SIZE: 0.5 mg
ANTIBODY CONCENTRATION: 6.4 mg/ml (by UV absorbance at 280 nm)
BUFFER: 0.125M Sodium Borate, 0.075M Sodium Chloride, 0.005M EDTA; ph 8.0
STABILIZER: None
PRESERVATIVE: 0.015 sodium azide
RECOMMENDED DILUTIONS: Western Blots 1:5,000 - 1:10,000
ELISA 1:4,000 - 1:8,000
Immunohistochemistry 1:50 - 1:200
APPLICATIONS: ELISA, Western Blotting, Immunochemistry.
STORAGE: Store vial at 4`C before opening. Aliquot and freeze.Avoid cycles
of thawing and freezing. Expiration date is six (6) months from opening.
PURITY: This product has been prepared by immunoaffinity chromatography using
immobilized antigens. Extensive cross-absorption was performed against other
collagens, human serum proteins and non-collagen extracellular matrix proteins
to remove any unwanted specificities. Typically less than 1% cross reactivity
against other types of collagen was detected using ELISA. Some class specific
anti-collagens may be specific for three-dimensional epitopes which may result
in diminished reactivity with denatured collagen or formalin-fixed, paraffin
embedded tissues.
SPECIFICITY: Antibody reacts with most mammalian Type IV collagens and has negligible cross-reactivity with Type I, II, III, V and VI collagens.
IMMUNOGEN: Collagen Type IV from human and bovine tissuee
NOTE: This product is FOR IN-VITRO Research USE ONLY
RDI Divison of researchd Industries Intl
San Jose, 95123 CA Snell ave 658
USA
or 408-780-0908
EMAIL:margaret@cellular-research.com
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