rev:  October 14, 2003

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ANTIBODIES  

(anti-Human and others as indicated)

RDI Divison of Fitzgerald Industries Intl  offers a wide line of  antibodies. Since no one antibody works best for all applications (neutralization, blotting, histochemistry, ELISA, etc), we offer many different types of antibodies to help solve this problem. Please inquire for other applications or types of antibodies not listed below.


also available: rabbit anti-collagen Type 1, Type 2, Type 3, Type 4, Type 5, Type 6 antibodies. . see collagen abs
Mouse anti-Collagen Type IV (COCKTAIL)   (human)

cat# RDI-COLL4abm-COC $406.00/0.5ml

        RDI-COLL4abm-COC1 $562.00/1ml

Presentation: Tissue culture supernatant with 0.09% sodium azide,

Isotype: mIgG1

Clone: PHM-12 & CIV 22

Reactivity: human (others not tested)

Uses: Immunohistology (Formalin/paraffin) (Use Ab at 1:50-1:100 for 30 min at RT) (Staining of formalin/paraffin tissues is enhanced by digestion of tissue sections with Protease XXV at 1mg/ml PBS for 5 minutes at 37°C

Positive Control: Skin.

Localization: Basement membrane

Background: Collagen IV is a major constituent of the basement membranes along with laminins and enactins. It is composed of á 1(IV) chain and á 2(IV) chain in 2:1 ratio . It can form insoluble fibers with high tensile strength. Antibody to collagen IV is useful in detecting the loss of parts of basement membrane in carcinomas. This MAB cocktail is highly specific to type IV collagen. In kidney, it reacts with glomerular and tubular basement membranes, parts of mesangial matrix and the Bowman’s capsule. It also reacts with basal lamina of capillaries as well as basement membranes in a variety of tissues. This Mab cocktail is excellent for the staining of routine formalin-fixed, paraffin-embedded tissues.

References: 1. Odermatt B F, et. al. 91984) Proc Natl Acad Sci USA, 81:7343-7347.

2. Lee CS; et al. Pathology, 1996, 28(2):135-8.

3. Delaigue O; et al. Graefes Archive for Clinical and Experimental Ophthalmology, 1995 233:

331-8.

Storage: 2-8 deg c

For Research Use Only

-individual abs/clones available on request


Mouse ANTI-COLLAGEN TYPE IV

Mab to Collagen Type IV      
cat#RDI-PRO10710 $344.00/vial


Clone 1042


Category Mouse monoclonal, ascites


Ig Class IgG2b


Immunogen Human placental type IV collagen


Clinical Application/Specificity Antibody 1042 is reactive with human collagen type IV of basement membranes, and shows a homogeneous staining pattern in all tissues. As neoplastic cells of invasive carcinomas often lack a continuous basement membrane, the antibody is useful to distinguish between non-invasive and invasive lesions. Additionally, it can be used for the differentiation of bullous lesions in dermatopathology. No cross-reactivity with other collagens.

Recommended Positive Control: for Skin, kidney


Application
· ELISA

· Immunoblotting

· Immunohistochemistry

· Suitable for frozen tissue and paraffin* sections; (*proteolytic treatment required; enzyme: pronase)

Antigen Recognized :Human specific

Working Dilution 1:10 - 1:20


Dilution Buffer PBS with 1% BSA and 0.1% Na-Azide


Incubation Time 1 h at room temperature


Storage At 4°C (undiluted) or at -20°C (aliquots)


Volume 1 ml


References

M.G. Havenitz, J.P.M. Cleutjens, C. Beek, E.v.d.Linden, A.F.P.M. De Goeij, and F.T. Bosman: Human specific anti-type IV collagen monoclonal antibodies, characterization and immunohistochemical applicatiuon. Histochemistry (1987) 87:123-128.

For In Vitro Research Use Only-Not For Use in Diagnostics


Pab to Collagen Type IV

cat#RDI-PRO10760 $344.00/vial


Category Rabbit serum


Ig Class IgG


Immunogen Human placental type IV collagen


Clinical Application/Specificity The antibody is reactive with collagen type IV of basement membranes, and shows a homogeneous staining pattern in all tissues. As neoplastic cells of invasive carcinomas often lack a continuous basement membrane, the antibody is useful to distinguish between non-invasive and invasive lesions. Additionally, it can be used for the differentiation of bullous lesions in dermatopathology. No cross reactivity with other collagens at optimal dilutions. In immunoblotting1, a slight cross reactivity with collagen type V is observed.

Recommended Positive Control for Skin, kidney

Application ELISA

Immunoblotting1

Immunohistochemistry

Suitable for frozen tissue and paraffin* sections; (*proteolytic treatment required; enzyme: pepsin)


Antigen Recognized in species tested so far: Most species incl. human, rat, mouse, rabbit


Working Dilution 1:50 - 1:100


Dilution Buffer PBS with 1% BSA and 0.1% Na-Azide


Incubation Time 1 h at room temperature or over night at 4°C


Storage At 4°C (undiluted) or at -20°C (aliquots)


Volume 0,25 ml

References

1. F.T. Bosman, M. Havenith, and J.P.M. Cleutjens: Basement Membranes in Cancer. Ustrastructural Pathology (1985) 8:291-304.

2. R. Visser, J.M.H. v.d. Beek, M.G. Havenith, J.P.M. Cleutjens & F.T. Bosman: Immunocytochemical detection of basement membrane antigens in the histopathological evaluation of laryngeal dysplasia and neoplasia.

For  In Vitro Research Use Only-Not For Use In Diagnostics


PRODUCT SPECIFICATION SHEET:Rabbit anti-Collagen Type IV

PRODUCT: Affinity Purified Anti-Collagen Type IV (Rabbit) Minimum Cross Reaction to Type I, II, III, V and VI Collagens.

CODE NO.: RDI-600-401-106   $562.00/vial


LOT NO:


PHYSICAL STATE: Liquid (Sterile Filtered)

SIZE: 0.5 mg


ANTIBODY CONCENTRATION: 6.4 mg/ml (by UV absorbance at 280 nm)


BUFFER: 0.125M Sodium Borate, 0.075M Sodium Chloride, 0.005M EDTA; ph 8.0


STABILIZER: None


PRESERVATIVE:  0.015 sodium azide


RECOMMENDED DILUTIONS: Western Blots 1:5,000 - 1:10,000

                                                       ELISA 1:4,000 - 1:8,000

                                                       Immunohistochemistry 1:50 - 1:200


APPLICATIONS: ELISA, Western Blotting, Immunochemistry.


STORAGE: Store vial at 4`C before opening. Aliquot and freeze.Avoid cycles of thawing and freezing. Expiration date is six (6) months from opening.


PURITY: This product has been prepared by immunoaffinity chromatography using immobilized antigens. Extensive cross-absorption was performed against other collagens, human serum proteins and non-collagen extracellular matrix proteins to remove any unwanted specificities. Typically less than 1% cross reactivity against other types of collagen was detected using ELISA. Some class specific anti-collagens may be specific for three-dimensional epitopes which may result in diminished reactivity with denatured collagen or formalin-fixed, paraffin embedded tissues.

SPECIFICITY: Antibody reacts with most mammalian Type IV collagens and has negligible cross-reactivity with Type I, II, III, V and VI collagens.


IMMUNOGEN: Collagen Type IV from human and bovine tissuee

NOTE: This product is FOR IN-VITRO Research USE ONLY


RDI Divison of Fitzgerald Industries Intl

34 Junction Square Drive

Concord MA 01742-3049

USA

phone (978) 371-6446 or (800) 370-2222

fax     (978) 371-2266

EMAIL:antibodies@fitzgerald-fii.com

Ordering terms

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