rev: January 20, 2003

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ANTIBODIES  

(anti-Human and others as indicated)

RDI Divison of Fitzgerald Industries Intl  offers a wide line of  antibodies. Since no one antibody works best for all applications (neutralization, blotting, histochemistry, ELISA, etc), we offer many different types of antibodies to help solve this problem. Please inquire for other applications or types of antibodies not listed below.


HPV Type 16 (see 4 clones below)

see also other HPV abs


Monoclonal mouse anti-E7 protein of human Papillomavirus type 16 (HPV)

Catalogue # RDI-TRK3HP1-281     (for clone#716-281)    

                    RDI-TRK3HP1-332     (for clone#716-332)

$375.00/mg   $312.00/mg 10+  Bulk quotes on request

Clones: 716-281, 716-332   These clones have been derived from hybridization of SP2/0 myeloma cells with spleen cells of Balb/c mice immunised with clones 716-281; 716-332

Mouse isotype: 716-281-IgG2a, 716-332-IgG2b

Application: Mab 716-281 is recommended to be used as a capture antibody and 716-332 as a conjugate in sandwich ELISA. Both clones are suitable for Western Blotting. Both antibodies are cross-reacting with E7 protein of HPV type 18.

Purification: Purified by chromatography on protein G Sepharose. Purity is tested by electrophoresis.

Presentation: Each vial contains antibodies in PBS, pH 7,2, containing 0,1% of sodium azide as preservative. Store at - 20 °C.

Material safety note: This product is sold as an antibody preparation for research purpose only. Standard Laboratory Practices should be followed when handling this material. Contains sodium azide (0,1%) as preservative. Although the amount of sodium azide is very small appropriate care must be taken when handling this product.

For Research Use Only


Mouse ANTI-HPV-16 LI PROTEIN

PRODUCT CODE :RDI-CBL402    $375.00/vial   $312.00/vial 3 or more

CLONE: CamVir-1

ISOTYPE: IgG2a

SOURCE: Tissue culture supernatent

PURIFICATION METHOD: Protein A affinity chromatography.

SPECIFICITY: Late protein of human papilloma virus type 16. HPV-6 & -II positive biopsies gave negative results. In western blotting CamVir-1 detects a 41kD protein that is observed in a wide range of uninfected mammalian cells, this unwanted specifity is absent by immunoprecipitation, immunofluorescence or immunoperoxidase. This may be a reflection of cross-reactivity between HPV-16 L1 and a cryptic epitope in mammalian cells that is only revealed following exhaustive denaturation.


APPLICATIONS:

* Detection of HPV in cervical smears and biopsies.

* CamVir-1 recognises HPV-16 LI by immunoprecipitation, western blotting, immunofluorescence on cells fixed in acetone or paraformaldehyde and immunoperoxidase staining of paraffin sections.

* Application to diagnostic kits.


REFERENCES: Browne, L. et al. J. Gen. Virol. 69, 1263 - 69 (1988)


 

PRESENTATION: Each monoclonal is delivered in a sealed vial at a concentration of 200µg/2ml in phosphate buffered saline solution containing 10mM sodium azide and 1mg/ml BSA. We recommend that each laboratory determine an optimum working titre for use in its particular application.

STORAGE: Purified monoclonal antibodies are generally stable for long periods at 4oC. However repeated warming to room temperature and re-cooling may result in a loss of activity and hence effective working titre. It is recommended that monoclonal antibodies are aliquotted upon receipt and then stored at -20C. After thawing they should then be used as a stock solution for up to 60 days. Dilute working solutions are best prepared on the day of use.


For research use only. Not supplied for use in human diagnostic or therapeutic procedures


RDI Divison of Fitzgerald Industries Intl

34 Junction Square Drive

Concord MA 01742-3049

USA

phone (800) 370-2222 or (972) 584-7093

fax     (978) 371-2266

EMAIL:antibodies@fitzgerald-fii.com

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