rev:    June 3, 2003

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ANTIBODIES  

(anti-Human and others as indicated)

RDI Divison of Fitzgerald Industries Intl  offers a wide line of  antibodies. Since no one antibody works best for all applications (neutralization, blotting, histochemistry, ELISA, etc), we offer many different types of antibodies to help solve this problem. Please inquire for other applications or types of antibodies not listed below.



MONOCLONAL ANTIBODIES TO p53 Oncoprotein:

-FOR RESEARCH USE ONLY :


available unlabelled or labelled as indicated -FT=FITC labeled   -BT-biotin labeled

custom conjugates may be available please inquire clone **=reactive on formalin fixed paraafin sections

see individual clone specs below chart
cat# description clone isotype size
RDI-CBL773 mouse anti-P53 human (aa250-270) DO-12 mIgG2b   200ug/2ml  $375.00
RDI-CBL774 mouse anti-p53 human (56-65) DO-14 ** mIgG1 200ug/2ml $375.00
RDI-CBL423 mouse anti-p53 human C term

(aa370-378)

Pab122 mIgG2b 200ug/2ml $375.00
RDI-CBL423BT mouse anti-p53 human C term:Biotin

(aa370-378)

Pab122 mIgG2b 100tests $375.00
RDI-CBL423FT mouse anti-p53 human C term:FITC

(aa370-378)

Pab122 mIgG2b 100tests $375.00
RDI-CBL422 mouse anti-p53 human N term

(aa20-25)

BP53-12 ** mIgG2a 200ug/2ml $375.00
RDI-CBL422BT mouse anti-p53 human N term:Biotin

(aa20-25)

BP53-12 mIgG2a 100 tests $375.00
RDI-CBL422FT mouse anti-p53 human N term:FITC

(aa20-25)

BP53-12 mIgG2a 100 tests $375.00
RDI-CBL420 mouse anti-p53 human N term

(aa32-79)

Pab1801 ** mIgG1 200ug/2ml $375.00
RDI-CBL420BT mouse anti-p53 human N term:FITC

(aa32-79)

PAB1801** mIgG1 100T $375.00
RDI-CBL420FT mouse anti-p53 human N term:FITC

(aa32-79)

Pab1801 mIgG1 100 tests $375.00
RDI-CBL404 mouse anti-p53 human (aa213-217) Pab240 mIgG1 200ug/2ml $375.00
RDI-CBL404FT mouse anti-p53 human :FITC

(aa213-217)

Pab240 mIgG1 100 tests $375.00
RDI-CBL404BT mouse anti-p53 human :Biotin

(aa213-217)

PAB240 mIgG1 100T $375.00
RDI-CBL405 mouse anti-mouse p53 (aa85-109) Pab246 mIgG1 200ug/2ml $375.00
RDI-CBL405BT mouse anti-mouse p53:Biotin Pab246 mIgG1 100 tests $375.00
RDI-CBL405FT mouse anti-human p53:FITC Pab246 mIgG1 100 tests $375.00

cat#                                 RDI-CBL404               RDI-CBL405                 RDI-CBL420              RDI-CBL422

clone#                              Pab240                            Pab246                           PAb1801                      Pab53-12

reactive formalin fixed tissue    YES                                   NO                                 YES                               YES

western blotting on

1) human wild type            YES                                  NO                                YES                                YES

2) mouse wildtype             YES                                  NO                                 NO                                 NO

3) human mutant                YES *                               NO                                YES                                YES

4) mouse mutant                YES                                  NO                                 NO                                 NO

IMMUNOPRECIPITATION:

1) human wildtype             NO                                   NO                                 YES                               YES

2) mouse wild-type            NO                                  YES                                NO                                 NO

3) human mutant                YES                                 NO                                 YES                               YES

4) mouse mutant                YES                                 NO                                 NO                                 NO

                                             $375.00                           $375.00                          $375.00                          $438.00
   
                                      (200ug purified)                         (200ug purified)                        (200ug purified)            (tissue supernatant)

-order with suffix-FT for FITC labeled or -BT for Biotin labeled Price: $375.00/100 test labeled vial

*some studies indicate clone pab240 only reactive with mutant in western blot under non-denaturing conditons, but reactive with both mutant and wildtype under denaturing conditions.


MOUSE MONOCLONAL ANTIBODY TO: HUMAN p53 ONCOPROTEIN

PRODUCT CODE                                   RDI-CBL773      $375.00

CLONE                                                    DO-12

ISOTYPE                                                 IgG1

IMMUNIZING ANTIGEN                       Human p53

FUSION PARTNER                                SP2.0/Ag14 myeloma cell line

SOURCE                                                  Mouse ascitic fluid

PURIFICATION METHOD                      Protein A affinity chromatography

SPECIFICITY                                        This antibody reacts with human p53 protein, wild type and mutant forms under denaturing conditions. It also reacts with mutant form under non-denaturing conditions but not with wild-type p53 protein under these conditions. In man the p53 gene is located on the small arm of chromosome 17. Alterations to the p53 tumour suppressor gene are a common feature of human malignancies, a normal function which, is to induce apoptosis after DNA damage and so its inactivation can permit the survival of cells that have sustained genetic damage. CBL 773 reacts with the human form of p53 protein at an epitope within the core region between amino acids 250 and 270 which is located between conserved domain IV and V on an exposed turn of the protein structure.

APPLICATIONS                                       *Western blotting

KNOWN SPECIESCROSS REACTIVITY  Known to cross-react with mouse p53 (amino acids 250-270)

REFERENCES                                           Vojtesek, B. et al. Oncogene 10, 389-393 (1995)

LOT SPECIFIC DATA

PRESENTATION The monoclonal is presented at a concentration of 200µg/2ml in phosphate buffered saline containing 10mM sodium azide and 1mg/ml bovine serum albumin. We recommend that each laboratory determine an optimum working titre for use in its particular application.

STORAGE For use within 1 month of purchase store at +4oC, for long term storage aliquot antibody into small volumes and store at -20oC.

For Research Use Only. Not supplied for use in diagnostic or therapeutic procedures


MOUSE MONOCLONAL ANTIBODY TO: HUMAN p53 ONCOPROTEIN

PRODUCT CODE                                      RDI-CBL774       $375.00

CLONE                                                       DO-14

ISOTYPE                                                    IgG1

IMMUNIZING ANTIGEN                         Human p53 protein

FUSION PARTNER                                   SP2.0/Ag14 myeloma cell line

SOURCE                                                    Mouse ascitic fluid

PURIFICATION METHOD                        Protein A affinity chromatography

SPECIFICITY                                             This antibody reacts with human p53 protein, wild type and mutant forms under denaturing and non-denaturing conditions. It specifically maps the n-terminus region of the p53 molecule (amino acids 56 to 65). CBL 774 has been used to study p53 protein changes in conformations and their association with different biological activities. Such conformation or changes may be important for the normal regulation of p53 function. The antibody also recognizes an additional 40 kDa band to the 53 kDa band expressed by the HOS cell line when used in Western blotting, database searches have failed to identify a protein other than p53 which contains the additional sequence recognized.

APPLICATIONS                                       For use on formalin fixed paraffin wax embedded tissue sections following heat induced epitope retrieval (HIER)*Frozen sections following methanol or acetone fixation*Western blotting.

REFERENCES                                         Vojtesek, B. et al. Oncogene 10, 389-393 (1995)

LOT SPECIFIC DATA

PRESENTATION                                    The monoclonal is presented at a concentration of 200µg/2ml in phosphate buffered saline containing 10mM sodium azide and 1mg/ml bovine serum albumin. We recommend that each laboratory determine an optimum working titre for use in its particular application.

STORAGE For use within 1 month of purchase store at +4oC, for long term storage aliquot antibody into small volumes and store at -20oC.

For Research Use Only. Not supplied for use in diagnostic or therapeutic procedures.


MOUSE MONOCLONAL ANTIBODY TO: HUMAN p53 ONCOGENE

PRODUCT CODE                 RDI-CBL423     $375.00

CLONE                                   pAb 122

ISOTYPE                                IgG2b

SOURCE                                 Tissue culture supernatant

PURIFICATION METHOD    Protein A affinity chromatography

SPECIFICITY                        This antibody reacts with a conserved, denaturation-resistant determinant of the p53 protein. The antibody maps on epitope of C-terminal amino acids 370-378 of p53.

APPLICATIONS      *The antibody will immunoprecipitate the p53 oncoprotein complexed with SV40 large T                                                    antigen*Flow cytometric studies*Immunofluorescence studies

REFERENCES           Harlow, E et al. J. Virol. 39, 861-70 (1981)Gannon, J.V. et al. Embo J. 9, 1595-1602 (1990)Banks, L. et al. Eur. J. Biochem. 159, 529-534 (1986)

KNOWN SPECIESCROSS REACTIVITY   Known to cross-react with monkey, mouse, rat and hamster

PRESENTATION The monoclonal is presented at a concentration of 200µg/2ml in phosphate buffered saline containing 10mM sodium azide and 1mg/ml bovine serum albumin. We recommend that each laboratory determine an optimum working titre for use in its particular application.

STORAGE For use within 1 month of purchase store at +4oC, for long term storage aliquot antibody into small volumes and store at -20oC.

Also available:

Cat#RDI-CBL423BT Biotin conjugated $375.00

Cat#RDI-CBL423FT FITC conjugated $375.00

For Research Use Only. Not supplied for use in diagnostic or therapeutic procedures


Mouse ANTI-p53 HUMAN ONCOGENE

PRODUCT CODE :   RDI-CBL404     $375.00/vial     $312.00/vial 3+

also available: Fitc labeled     cat#RDI-CBL404FT   $375.00/100T

                      biotin labeled  cat#RDI-CBL404BT   $375.00/100T inquire for availability

CLONE                                  PAb 240

ISOTYPE                               IgG1

SOURCE                                Tissue culture supernatant

PURIFICATION METHOD    Protein A affinity chromatography

SPECIFICITY                         This antibody is specific for human p53 (antigen molecular weight 50 kDa).

APPLICATIONS                    * Detection of p53 oncogene protein* Detection of mutant p53* Prevalence of detection using CBL 404-50% colon carcinoma sections positive (30 samples)-70% lung carcinoma sections positive (50 samples)-30% carcinoma breast samples positive (50 samples)* Normal and pre-malignant tissues negative* Reacts on methacarn fixed tissue

KNOWN SPECIES CROSS REACTIVITY This antibody is known to cross react with mouse, rat, chicken, monkey, cow and hamster tissues.

REFERENCES                      Iggo et al. Lancet 335, 675-679 (1990)Gannon et al. EMBO J. 4, 1-8 (1990)

PRESENTATION The monoclonal is presented at a concentration of 200µg/2ml in phosphate buffered saline containing 10mM sodium azide and 1mg/ml bovine serum albumin. We recommend that each laboratory determine an optimum working titre for use in its particular application.

STORAGE For use within 1 month of purchase store at +4oC, for long term storage aliquot antibody into small volumes and store at -20oC.

also available: Fitc labeled cat#RDI-CBL404FT $375.00/100T

                     biotin labeled cat#RDI-CBL404BT $375.00/100T

For research use only. Not supplied for use in human diagnostic or therapeutic procedures


Mouse ANTI-p53 WILD TYPE ONCOGENE PROTEIN

PRODUCT CODE :RDI-CBL405     $375.00/vial    $312.00 3 or more

    also available FITC labeled,   cat#RDI-CBL405FT  $375.00/100T

                         Biotin labeled   cat#RDI-CBL405BT  $375.0/100T

CLONE   PAb 246

ISOTYPE  IgG1

SOURCE  Tissue culture supernatant

PURIFICATION METHOD  Protein A affinity chromatography

SPECIFICITY   This antibody is specific for p53 mouse (wild type only) oncogene protein, molecular weight 50 kDa.

APPLICATIONS

* Research reagent

REFERENCES Gannon et al. EMBO J. 4, 1-8 (1990)

                          Yewpell et al. J. Virol. 59, 444-452 (1986)

PRESENTATION

The monoclonal is presented at a concentration of 200µg/2ml in phosphate buffered saline containing 10mM sodium azide and 1mg/ml bovine serum albumin. We recommend that each laboratory determine an optimum working titre for use in its particular application.

STORAGE:   For use within 1 month of purchase store at +4oC, for long term storage aliquot antibody into small volumes and store at -20oC.

For Research Use Only. Not supplied for use in diagnostic or therapeutic procedures.


Mouse ANTI-p53 HUMAN ONCOGENE

PRODUCT CODE                        RDI-CBL420         $375.00/200ug

CLONE                                         Pab 1801

ISOTYPE                                       IgG1

SOURCE                                        Tissue culture supernatant

PURIFICATION METHOD           Protein A affinity chromatography

SPECIFICITY                                Human p53 (wild and mutant forms)

APPLICATIONS                            *Studies of the mutation of the p53 protein which may represent the commonest genetic event in human malignancy*Expression correlates with poor prognosis in breast cancer*In colonic cancer p53 expression seen in 47% of tumours and 9% of colonomas*Antibody reacts with formalin-fixed paraffin wax embedded tissue sections which have been microwave treated

REFERENCES Banks, L. et al. Eur. J. Biochem. 155, 529-34 (1981)Iggo, R. et al. Lancet 335, 675-79 (1990)Cattoretti, G. et al. Int. J. Canc. 41, 178-83 (1988)Lane, D. P. & Benchimol, S. Genes & Develop. 4, 1-8 (1990)

PRESENTATION The monoclonal is presented at a concentration of 200µg/2ml in phosphate buffered saline containing 10mM sodium azide and 1mg/ml bovine serum albumin. We recommend that each laboratory determine an optimum working titre for use in its particular application.

STORAGE For use within 1 month of purchase store at +4oC, for long term storage aliquot antibody into small volumes and store at -20oC.

Also available: FITC labeled cat#Rdi-CBL420FT $375.00/100T

Biotin labeled cat#RDI-CBL420BT $375.00/100T

Bulk material upon request

This clone is listed as not reacting with rat or mouse


For research use only. Not supplied for use in human diagnostic or therapeutic procedures


Mouse ANTI-p53 HUMAN ONCOGENE

PRODUCT CODE RDI-CBL422     $375.00/vial

CLONE                                    BP53-12

ISOTYPE                                 IgG2a

SOURCE                                  Tissue culture supernatant

PURIFICATION METHOD     Protein A affinity chromatography

SPECIFICITY                          This antibody reacts with the 53 kDa nuclear protein as demonstrated by immunocytochemistry and immunoblotting.

APPLICATIONS                     *Research of p53 tumour suppressor gene function and abnormalities in cancer*Detection of p53 over expression/mutations in clinical specimens, association of p53 abnormalities with higher grade tumours*Studies as a possible marker in differential diagnosis and prognosis of p53 positive versus p53 negative human tumours

REFERENCES                       Harlow, E et al. J. Virol. 39, 861-70 (1981)Gannon, J.V. et al. Embo J. 9, 1595-1602 (1990)Banks, L. et al. Eur. J. Biochem. 159, 529-534 (1986)

PRESENTATION The monoclonal is presented at a concentration of 200µg/2ml in phosphate buffered saline containing 10mM sodium azide and 1mg/ml bovine serum albumin. We recommend that each laboratory determine an optimum working titre for use in its particular application.

STORAGE For use within 1 month of purchase store at +4oC, for long term storage aliquot antibody into small volumes and store at -20oC.

Also available: FITC labeled cat#RDI-CBL422FT $375.00/100T

                          Biotin labeled cat#RDI-CBL422BT $375.00/100T

Bulk Quotes on request

-listed as non reactive with mouse and rat

For Research Use Only. Not supplied for use in diagnostic or therapeutic procedures.


RDI Divison of Fitzgerald Industries Intl

34 Junction Square Drive

Concord MA 01742-3049

USA

phone (978) 371-6446 or (800) 370-2222

fax     (978) 371-2266

EMAIL:antibodies@fitzgerald-fii.com

Ordering terms

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