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ANTIBODIES  

(anti-Human and others as indicated)

RDI Divison of Fitzgerald Industries Intl  offers a wide line of  antibodies. Since no one antibody works best for all applications (neutralization, blotting, histochemistry, ELISA, etc), we offer many different types of antibodies to help solve this problem. Please inquire for other applications or types of antibodies not listed below.

PRODUCT CODE :RDI-CBL188
$375.00/vial    $338.00/vial 3 or more

CLONE: CHC5.1

ISOTYPE: IgM

SOURCE: Mouse ascitic fluid.

Antigen: Coated vesicles (clathrin) of bovine brain

PURIFICATION  METHOD: Ammonium sulphate precipitation followed by gel filtration.

SPECIFICITY: Clathrin heavy chain from diverse tissues of, for instance, bovine, rat, human and amphibian origin. Reacts with a polypeptide of MR 180,000 (clathrin heavy chain) from coated vesicles of various tissues (brain, mammary gland, ovaries). Reacts on cultured cells line including HeLa, SV-40 & RVF-SMC.


APPLICATIONS: * Marker for detection of receptor mediated endocytosis.

                               * Suitable for frozen sections

                               * Western blotting

REFERENCES: Bruder, G. and Wiedenmann, B. Exp. Cell Res. 164, 449-62 (1986)

PRESENTATION: Each monoclonal is delivered in a sealed vial at a concentration of 100µg lyophilysed. Reconstitute with 1ml distilled water. We recommend that each laboratory determine an optimum working titre for use in its particular application. Use approx 1:10 dilution for immunohistochemical use (1 hr at RT).

STORAGE: Purified monoclonal antibodies are generally stable for long periods at 4oC. However repeated warming to room temperature and re-cooling may result in a loss of activity and hence effective working titre. It is recommended that monoclonal antibodies are aliquotted upon receipt and then stored at -20 DEG C. After thawing they should then be used as a stock solution for up to 30 days. Dilute working solutions are best prepared on the day of use.

For research use only. Not supplied for use in human diagnostic or therapeutic procedures

cat# RDI-CLATHRabm         $438.00/vial 50ug

        RDI-CLATHRabm-1     $750.00/vial 150ug

Background: Clathrin is the major protein component in the coat formed around pits and vesicles involved in receptor-mediated endocytosis. Clathrin forms a non covalently bound triskeleton structure composed of three heavy chains (Mr=192kda) and three light chains (Mr=23-25kDa). Each leg of the triskeleton structure contains one heavy and one light chain. The three heavy chains forming the triskeleton structure are attached at their respective proximal ends like spokes on a wheel. Clathrin heavy chain is composed of a terminal globular domain, a distal segment containing several sensitive to enzymatic cleavage, and a proximal segment which contains a light binding site. The proximal and distal domains are connected by a joint segment at which there is a sharp bend in the heavy chains of fully assembled triskelia. Although the calculated Mr is 192kDa, clathrin heavy chain migrates at approximately 180kDa by PAGE.

isotype: mIgG1

package: 50ug (200ul) or 150ug (600ul) (at 0.25mg/ml in 50% glycerol, PBS + 1.5mM NaN3 & 1mg/ml BSA)

antigen: N-terminal protein fragment corresponding to amino acids 4-171 of rat clathrin heavy chain (located in the terminal globular domain).

USE: Suitable for western blot on human, rat, mouse and chick using approx 0.25 to 1ug/ml ab on total cell of HeLa cells. Major band =MW 180kDa.

Immunofluorescence ,immunohistochemistry & immunoprecipitation (native or denatured)

Storage: Store at -20 DEG C upon receipt. Recommend aliquoting. Avoid frequent warming cycles.

Main REF: -Liu, S.H et al, 1995:CELL 83:257

Precautions: For In vitro research Use Only. Not for use in or on humans or animals or for diagnostics. It is the responsibility of the user to comply with all local/state and Federal rules in the use of this product. We are not responsible for any patent infringements that might result with the use of or derivation of this product.

cat# RDI-CLATHRLCabmX     $438.00/100ug     $688.00/200ug

RDI-CLATHRLCabPX            $750.00/200ug   (No azide/no BSA prep)

Supplied: Purified antibody at 200ug/ml in PBS with 0.09% sodium azide and 0.2% BSA

Clone: CON.1

Isotype: mIgG2Bk

Specificity: Clathrin consists of three heavy chains (192kD) and three light chains (LCa or LCb, 25-29kD) and has the property of self assembly into a polyhedral cytoskeletal element. This self- assembly property facilitates clathrin's central role in various membrane sorting and selective transport steps within cells. Monoclonal antibody CON.1 reacts with clathrin light chains (CLC) LCa and LCb. It was raised using a peptide (residues 23-44 from bovine clathrin light chain) corresponding to the conserved region of CLC.

Reactivity: Cow, human, rat and yeast LCa and LCb

Uses: -immunoblotting 1-4ug/ml (predicted 30kda band)

         -immunofluorescence 5ug/ml (recommend PFA fixation)

        -immunoprecipitation 2ug/mg protein

       -histochemistry 5-10ug/ml

          Immunohistology (Formalin/paraffin) [Staining of formalin-fixed tissues REQUIRES boiling tissue sections in 10mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 min.]

positive Ctrl: Placenta

Staining: cell membrane abd cytoplasm

Storage: Store at 4 DEG C.

References: AP Jackson, H-F Seow,N. Holmes, K Drickamer, P Parham. Clathrin light chains contain brain-specific insertion sequences and a region of homology with intermediate filaments. Nature 326:154-159, 1987.

IS Nathke, J Henser, A Lupas, J Stock, CW Turk, FM Brodsky. Folding and trimerization of clathrin subunits at the triskelion hub. Cell 68:899-910, 1992.

(For In Vitro Research Use Only)

34 Junction Square Drive

Concord MA 01742-3049

USA

phone (978) 371-6446 or (800) 370-2222

fax     (978) 371-2266

EMAIL:antibodies@fitzgerald-fii.com

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