rev: April 6, 2004
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ANTIBODIES
(anti-Human and others as indicated)
RDI Divison of researchd Industries Intl offers a wide line of antibodies. Since no one antibody works best for all applications (neutralization, blotting, histochemistry, ELISA, etc), we offer many different types of antibodies to help solve this problem. Please inquire for other applications or types of antibodies not listed below.
Sheep Polyclonal Antibody TO 5'-BROMO-2'DEOXYURIDINE
-For Immunoprecipitation, histochemistry, brdU incporation -broad mammalian species reactivity
PRODUCT CODE : RDI-BRDUabS $438.00/vial
SOURCE: Sheep sera
Immunogen: Highly purified bromodeoxyuridine
PURIFICATION METHOD: DEAE chromatography
SPECIFICITY: very low cross reactivity to 5-methyl cytosine (see notes
below)
References:-Campana, D. et al, Dept of Immunology Royal Free Hospital School of Medicine, London U.K.
-Journal of Immunological Methods 107 (1):79-88, 1988 February 24.
PRESENTATION: delivered in a sealed vial at a concentration of 250µg
in 0.25ml PBS buffer (no Azide).We recommend that each laboratory determine
an optimum working titre for use in its particular application.
STORAGE: Purified antibodies are generally stable for long periods at 4oC.
However repeated warming to room temperature and re-cooling may result in
a loss of activity and hence effective working titre. It is recommended that
monoclonal antibodies are aliquotted upon receipt and then stored at -20C.
After thawing they should then be used as a stock solution for up to 60 days.
Dilute working solutions are best prepared on the day of use.
For research use only. Not supplied for use in human diagnostic or therapeutic
procedures
-fixation in cold methanol for 30 minutes followed by immersion in 0.007N NaOH for 10-15 seconds allows Brdu staining with simultaneous detection of nuclear, cytoplasmic and membrane antigens as well as preservation of morphological detail. This method is optimal for detection of nuclear Ki67 and TdT. -original work performed Dr. David Stoller, Tufts University, Boston Ma.
RDI Divison of researchd Industries Intl
San Jose, 95123 CA Snell ave 658
USA
or 408-780-0908
EMAIL:margaret@cellular-research.com
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