rev:     July 7, 2004

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  ANTIBODIES  

(anti-Human and others as indicated)

RDI Divison of Fitzgerald Industries Intl  offers a wide line of  antibodies. Since no one antibody works best for all applications (neutralization, blotting, ELISA, etc), we offer many different types of antibodies to help solve this problem. Please inquire for other applications or types of antibodies not listed below.

Cardiac Troponin T  (see also troponin brochure for more information)

Please email your request for the troponin Data Pack for more details on the various troponin products we have available. (  download  July 7, 2004 update:   http://www.researchd.com/troponin/troponindatapack.pdf

MONOCLONAL ANTI-TROPONIN T

catalog# RDI-4T19-xxxxx     where xxx=clone number (see below)

Price: $438.00/mg     $375.00/mg    2-9mg        Significant Bulk Discounts available!

clones available:  5C12, 1F2, 2F3, 7G7, 2G3, 6G9, 1A11, 9G6, 9G8, , 1C11, 1F11, 3D6

-For Elisa: Pairs: Coating/detection:

  1C11-9G6, 1C11-7F4, 7G7-6G9, 7G7-9G6, 7G7-1C11, 7G7-2F3, 1C11-6G9,

-for BIAcore: best capture abs: 1C11,  7A9,  9G6,  7G7

Custom Biotin labeling :  $562.00/mg 3-10  Larger sizes quoted on request, approx 3-4 weeks special order). add-BT to catalog number)  Mimimum 3mg per antibody
non labeled data:
packaging: 1mg of purified antibody at 1-10 mg/ml in PBS, pH 7.4, containing 0.1% of sodium azide as preservative. Store at 4 DE C.


Purification: Protein A sepharose Purity tested by electrophoresis

Clones(isotype): 5C12 (IgM), 1F2 (IgG1), 2F3 (IgG2b),

                         7G7 (IgG1), 2G3 (IgG2b), 6G9 (IgG1),

                         1A11 (IgG2b) , 9G6 (IgG1) , 9G8 (IgG1), 7F4 (IgG2B)

                         1C11(IgG1), 1F11(IgG2B), 3D6 (IgG1).   Clone 1C11 recommended for capture antibody in BIAcore(tm) assay

Specificity: see next page for clone specific data

Application: Troponin T immunoassay and immunoaffinity purification. For immunoassays, currently recommend sandwich pairing as follows (coating antibody-detecting ab); 1C11-7G7, 1C11-2F3, 7G7-6G9, 7G7-1C11, 7G7-2F3, 1C11- 6G9,

Reference: FEBS, Volume 315, 1, 25-28, 1993

All material is for in vitro research use only-not for use in or on humans or animals-not for diagnostics. Not responsiblefor any patent infringements for the use or derivation of these products.

Cardiac Troponin T specificity (other species) Western blot data

-epitope mapping data  troponin T monoclonals-please inquire-

-see next page  for titer data and other specificity information

(reactivity shown below from simple western blot tests, this does not mean that antibodies will not cross with the shown species under any conditions or at very high concentrations)

Clone Subclass epitope human bovine porcine goat canine rabbit cat rat mouse fish
1F2 IgG1 60-70 + + - + + + + + + +
2D10 IgG1 60-70 + - - - + - - - - -
2EG11 IgG2a 60-70 + + + + + + + - + -
7F4 IgG2b 60-70 + +   + +   - - - -
7G7 IgG1 60-70 + + - - - - - - - -
3D6 IgG1 70-94 + + - + + + - - + +
2F3 IgG2b + + + + + + + + + + + + +
2G3 IgG2b 94-180 + + + + + + + + + + -
6G9 IgG1 94-180 + + - + + + - + + +
1F11 IgG2b 146-160 + + + + + + + + + + + + + +
1A11 IgG2b 146-160 + + + + + + + + + + + + + + +
7A9 IgG1 180-287 + + - + + + + - - -
9G6 IgG1 + + + + + + + + + -
9G8 IgG1 + + + + + + - + + +
1C11 IgG1 + + + + + + + + + -
11GD5 IgG2a - + + + + + + - - -

Cardiac Troponin T monoclonals:specificity data

-For Elisa: Pairs: Coating/detection: 1C11-9G6, 1C11-7F4, 7G7-6G9, 7G7-9G6, 7G7-1C11, 7G7-2F3, 1C11-6G9,

-for BIAcore: best capture abs: 1C11, 7A9, 9G6, 7G7

Specificity: Clone 5C12 - human cardiac troponin T, bovine cardiac troponin T. Cross-reactivity with human skeletal muscle troponin T is less than 3%. Immunogen- bovine cardiac troponin T. Specific antibody titer in ELISA -10-6. mIgM   MoAb 5C12 weakly stains CNBr-peptides of skeletal   troponin T with Mr 10.5 kDa (residues 152-230) and effectively interacts with the homologous region of   cardiac troponin T (residues 180-258) included in   peptide with Mr 13 kDa.
Clone 1F2-human cardiac troponin T, bovine cardiac troponin T.There is no cross-reactivity with human skeletal troponin T. Immunogen - bovine cardiac troponin T. mIgG1 Specific antibody titer in ELISA - 3x10 -8. of cardiac troponin T.
Clone 2F3-human cardiac troponin T. Cross-reactivity with human skeletal troponin T is less than 0, 01%. Immunogen-human caradiac troponin T.Specific anti body titer in ELISA- 3x10-6. mIgG2b
Clone 7G7-human cardiac troponin T. There is no cross-reactivity with human skeletal troponin T. Immunogen-human cardiac troponin T.Specific antibody titer in ELISA- 5x10-6. mIgG1
Clone 2G3-human cardiac troponin T. Cross-reactivity with human skeletal troponin T is less than 0.01%. Immunogen-human cardiac troponin T. Specific antibody titer in ELISA- 7x10-6. mIgG2b
Clone 6G9-human cardiac troponin T, bovine cardiac troponin T. Cross-reactivity with human skeletal troponin T is less than 0.01%. Immunogen-bovine cardiac troponin T. Specific antibody titer in ELISA -5x10-6. mIgG1
Clone 1A11-human cardiac troponin T Cross-reactivity with human skeletal troponin T is less than 0.1%. Immunogen-human cardiac troponin T. Specific antibody titer in ELISA -5x10-6. mIgG2b
Clone 9G8-human cardiac troponin T   -Cross-reactivity with human skeletal troponin T is less than 0.1%. Immunogen-human cardiac troponin T. -Specific antibody titer in ELISA -10-6. mIgG1
Clone 1C11-human cardiac troponin T   -Cross-reactivity with human skeletal troponin T is less than 0.01%. Immunogen-human cardiac troponin T. -Specific antibody titer in ELISA -5x10-6. mIgG1
Clone 1F11-human cardiac troponin T   -Cross-reactivity with human skeletal troponin T is less than 0.01%. Immunogen-human cardiac troponin T. -Specific antibody titer in ELISA -5x10-6. mIgG2b
Clone 3D6-human cardiac troponin T -Cross-reactivity with human skeletal troponin T is less than 0.01%. Immunogen-human cardiac troponin T. -Specific antibody titer in ELISA -10-6. mIgG1
Clone 2D10-human cardiac troponin T, bovine cardiac troponin T, -Cross-reactivity with human skeletal troponin T is less than 0.1%. Immunogen-human cardiac troponin T. -Specific antibody titer in ELISA -3 X 10-5 mIgG1

-on backorder unitl at least Oct 98

Clone 7A9-human cardiac troponin T, bovine cardiac Troponin T -mIgG1 Cross-reactivity with human skeletal troponin T is less than 0.01%. Immunogen-human cardiac troponin T. -Specific antibody titer in ELISA -3 X 10-6. mIgG1
Clone 4D11-human cardiac troponin T, bovine cardiac troponin T -mIgG2b Cross-reactivity with human skeletal troponin T is 60%%. Immunogen-human cardiac troponin T. -Specific antibody titer in ELISA -1 X 10-6. mIgG2b
Clone 7F4-human cardiac troponin T, bovine cardiac troponin T -mIgG2b No Cross-reactivity with human skeletal troponin T. Immunogen-human cardiac troponin T. -Specific antibody titer in ELISA -3 X 10-6. mIgG2b

For In Vitro or Further Manufacturing Use Only-BULK QUOTES ON REQUEST.

RDI Divison of Fitzgerald Industries Intl

34 Junction Square Drive

Concord MA 01742-3049

USA

phone (978) 371-6446 or (800) 370-2222

fax     (978) 371-2266

EMAIL:antibodies@fitzgerald-fii.com

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