rev: July 7, 2004
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ANTIBODIES
(anti-Human and others as indicated)
RDI Division of Fitzgerald Industries Intl offers a wide line of antibodies. Since no one antibody works best for all applications (neutralization, blotting, ELISA, etc), we offer many different types of antibodies to help solve this problem. Please inquire for other applications or types of antibodies not listed below.
http://www.researchd.com/troponin/troponindatapack.pdf updated data pack July 7, 2004
catalog# RDI-TRK4T20-xxxx, xxx=clone#
Price: $438.00/mg $406.00/mg 3-9mg Significant Bulk Discounts available!
packaging: 1 milligram of purified antibody in 0.275ml (3.7mg/ml) PBS, pH
7.4, containing 0.1% of sodium azide as preservative. Store at 4 DEG C.
Purification: Protein A sepharose Purity tested by electrophoresis
Clone: Clone Sl1 (F8),
This clone has been derived from hybridization of SP2/0 myeloma cells with spleen cells of Balb/c mice immunized with troponin I from rabbit skeletal muscle.
2H9, 12F10, 6F11, 8B9, 11E6, 7G2
These clones were derived from hybridization of SP2/0 myeloma cells with spleen cells of Balb/c mice immunized with troponin I from human skeletal musle
Isotypes: IgG2b: SIL(F8), 2H9, 12F10, 7G2
IgG2a: 6F11, 8B9, 11E6
Specificity: Troponin I from human skeletal muscle Cross-reaction with the cardiac isoform in ELISA is about:
<0.01% clone F8,6F11,8B9,11E6
<0.05% 12F10
<1.5% 7G2
8% clone 2H9
-The best clones for for Western blotting with skeletal troponin I= 7G2 ,6f11, 8B9, 11E6
Use: Best pairs for Elisa: coating-conjugate 2H9-7G2 or 12F10-7G2
2H9-6F11, 2H9-8B9, 2H9-11E6, 12F10- 7G2, 12F10-6F11, 12F10-8B9, 12F10-11E6
Note: This product is sold as an antibody preparation for research purpose only. Standard Laboratory Practices should be followed when handling this material. Contains sodium azide (0.1%) as preservative. Although the amount of sodium azide is very small appropriate care must be taken when handling this product.
FOR IN VITRO RESEARCH USE ONLY
Note: Upon receipt, tighten cap and centrifuge for 1-2 minutes at 1-2K RPM to concentrate antibody in vial. If desired, add PBS with 0.1% NaN3 to bring concentration to 1mg/ml.
All material is for in vitro research use only-not for use in or on humans or animals-not for diagnostics. Not responsible for any patent infringements for the use or derivation of these products. s
DATA SHEET:Human Skeletal Muscle Troponin I
Catalog#: RDI-SkelTropI
Batch# see sheet with shipment
Package Size: 100 micrograms $688.00
500 micrograms $1750.00
1 milligram $2375.00
Supplied: lyophilized essentially salt free powder
Source: Human skeletal muscle
Purity: >98% by SDS-Page analysis (see spec sheet with shipment)
Storage: -20 DEG C upon receipt
Reconstitution: Reconstitute with distilled water.
USES: Standard in immunoassay, immunogen for antisera production, antigen
for iodination
Precautions: For In vitro research Use Only. Not for use in or on humans
or animals or for diagnostics . All starting material were tested and found
negative for HBsAg and HIV-1 antibodies by an FDA approved method. However,
all material should be handled at Biosafety Level II conditions and treated
as if capable of transmitting disease.
It is the responsibility of the user to comply with all local/state and Federal
rulesin the use of this product. We are not responsible for any patent
infringements that might result with the use of or derivation of this product.
Also available: Cardiac Troponin I and monoclonals
Cardiac Troponin T and monoclonals
Skeletal Troponin I, T and C antigens
RDI Division of Fitzgerald Industries Intl
34 Junction Square Drive
Concord MA 01742-3049
USA
phone (978) 371-6446 or (800) 370-2222
fax (978) 371-2266
EMAIL:antibodies@fitzgerald-fii.com
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