rev: February 17, 2005
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ANTIBODIES
(anti-Human and others as indicated)
RDI Division of Fitzgerald Industries Intl offers a wide line of antibodies. Since no one antibody works best for all applications (neutralization, blotting, ELISA, etc), we offer many different types of antibodies to help solve this problem. Please inquire for other applications or types of antibodies not listed below.
http://www.researchd.com/troponin/troponindatapack.pdf
CAT#: RDI-GPBBP-xxxx xxxx=clone#
$469.00/mg $438.00/mg 10mg+
BULK QUOTES ON REQUEST
Clone#/(isotype) 17B6 (IgG1), 10H5 (IgG2a), 1G6 (IgG2b),
3G1 (IgG1), 10D12 (IgG1), 5B8 (IgG1)
7B9 (IgG2b), 8G7 (IgG1), 6G5 (IgG1)
11C10 (IgG1), 6F1 (IgG1), 9F5 (IgG1)
These clones have been derived from hybridization of Sp 2/0 myeloma cells with spleen cells of Balb/c mice immunized with human GPBB.
Specificity: Clones 7B9, 8G7, 6G5, 11C10 and 1G6) are specific for the BB
isoenzyme. The other clones also recognize to varying extents the MM isoenzyme
as well. The best combination for Elisa are:
Capture Mab Detection MaB
1G6 6F1
1G6 9F5
1G6 17B6
6F1 7B9
17B6 10H5
Background: GPBB-sensitive marker for the AMI diagnosis within 4 hours after the onset of chest pain. It has also been shown that GPBB increased in a considerable proportion of AMI patients within 2-3 hhours from chest pain onset. GPBB increased early in patients with unstable angina. GPBB can be also a sensitive marker for the detection of peri-operative myocardial ischaemia and infraction in patients undergoing coronary artery bypass grafting.
Application: -Western blot: 3G1, 10H5, 10D12, 17B6
Purification: Purified by chromatography on protein A Sepharose. Purity is tested by electrophoresis.
Presentation: Each vial contains 1 milligram antibodies with concentration (see batch sheet) in PBS, pH 7.4, containing 0.1% of sodium azide as preservative. Store at +4C.
Material safety note: This product is sold as an antibody preparation for research purpose only. Standard Laboratory Practices should be followed when handling this material. Contains sodium azide (0.1%) as preservative. Although the amount of sodium azide is very small appropriate care must be taken when handling this product.
Custom Biotin or HRP Labeling quoted on request
(from human Heart Tissue)
cat# RDI-GPBBPP-AG $938.00/100ug
RDI-GPBB-AGX $2188.00/500ug RDI-GPBB-AGX1= $3750.00/1mg
GPBB-sensitive marker for the AMI diagnosis within 4 hours after the onset of chest pain. It has also been shown that GPBB increased in a considerable proportion of AMI patients within 2-3 hhours from chest pain onset. GPBB increased early in patients with unstable angina. GPBB can be also a sensitive marker for the detection of perioperative myocardial ischaemia and infraction in patients undergoing coronary artery bypass grafting.
Package: solution in 1mM beta-glycerophosphate, 1mM EDTA, 15mM ME, 0.5M NaCL ph7.8, 50% glycerol. water.
Purity: >95% as determined by a 10-20% gradient SDS-PAGE. The
immunological reactivity was confirmed with a monoclonal antibody
that is specific for the GPBB.
USE: Suitable as a standard in immunoassay for early detection of acute
myocardial infarction, as an immunogen for antisera production and as a tracer
for iodination.
Storage: Store at -20 DEG C. Avoid frequent freeze thaw cycles.
Warning: Made with human heart tissue. Blood samples from tissue donors were
tested and found negative for HbSAg, HIV-1 and HIV-2 antibodies and HCV.
Treat as if capable of transmitting infection Handle at Biosafety Level II
conditions.
For IN Vitro Research Use Only-Not For Use IN Or on Humans Or Animals.
34 Junction Square Drive
Concord MA 01742-3049
USA
phone (978) 371-6446 or (800) 370-2222
fax (978) 371-2266
EMAIL:antibodies@fitzgerald-fii.com
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