rev:  August 6, 2004 (under construction-on line specs in process)

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ANTI-SHEEP/OVINE

CD CLUSTERED

& OTHER

ANTIBODIES  

RDI Divison of Fitzgerald Industries Intl  offers a wide line of  antibodies. Since no one antibody works best for all applications (flow cytometry, neutralization, blotting, histochemistry, ELISA, etc), we offer many different types of antibodies to help solve this problem. Please inquire for other applications or types of antibodies not listed below. All products are for in vitro research use only-not for use in or on humans or animals-not for use in diagnostics. Price/availability/specifications subject to change without notice.

please email for others not listed below or "for non activated links " shown below:

Price, specifications and availability subject to change without notice. We are not responsible for any patent infringements that might result from theuse or derivation of these or any products ALL PRODUCTS ARE FOR IN VITRO RESEARCH USE ONLY-NOT FOR USE IN OR ON HUMANS OR ANIMALS-NOT FOR DIAGNOSTICS.

Catalog#: RDI-SHMACROabm-63     $438.00/vial

Package Size: 2ml tissue culture superntant with 0.2M Tris/Hcl pH7.4 and 5-10% fetal calf serum and 10mM sodium azide

Antigen: sheep macrophages

Clone: VPM 63

Isotype: mIgG1

Specificity: recognizes an antigen of 42/44kd expressed by most tissue macrophages

USE: -flow cytometry (1:3 dilution)

         -histochemsitry (acetone fixed frozen   sections)1:2

         -immunoprecipitation (neat)

       -not tested for other applications

Titer must be optimized for each method

Storage: Store at 4 DEG C (short term,) -20 DEG C long term. Avoid frequent freeze thaw cycles.

Precautions: For In vitro research Use Only. Not for use in or on humans or animals or for diagnosticsIt is the responsibility of the user to comply with all local/state and Federal rules in the use of this product. We are not responsible for any patent infringements that might result with the use of or derivation of this product.

see also clone VPM64

Catalog#: RDI-SHMACROabm-64   $438.00/vial

Package Size: 2ml tissue culture superntant with 10mM sodium azide

Antigen: sheep macrophages

Clone: VPM 64

Isotype: mIgG1

Specificity: recognizes a cell surface antigen of 66- 81kd, strongly expressed by ovine alveolar macrophages and peripheral blood granulocytes, and more weakly by monocytes and other tissue macrophages.

USE: -flow cytometry (1:2 dilution)

       -histochemistry (acetone fixed frozen sections)

       -immunoprecipitation (neat)

-not tested for other applications

Titer must be optimized for each method

Storage: Store at 4 DEG C (short term,) -20 DEG C long term. Avoid frequent freeze thaw cycles.

Precautions: For In vitro research Use Only. Not for use in or on humans or animals or for diagnostics It is the responsibility of the user to comply with all local/state and Federal rules in the use of this product. We are not responsible for any patent infringements that might result with the use of or derivation of this product.

For Research Use Only

Catalog#: RDI-SHCD8abm-31   $438.00/vial

Package Size: 2ml tissue culture supernatant with 0.2M Tris/HCL pH7.4, 5-10% fetal calf serum and 0.01% sodium azide

Antigen: sheep thymocytes

Clone: VPM31

Isotype: mIgM (recommend use of specific anti-IgM secondary antibody)

Approx protein: 7-10mg/ml total protein concentration

Specificity: recognizes the sheep CD8 cell surface antigen expressed by the cytotoxic/supressor subset of T cells.

USE: -flow cytometry (neat)

        -histochemsitry (acetone fixed frozen  sections)

       -not tested for other applications

Titer must be optimized for each method

Storage: Store at 4 DEG C (short term,) -20 DEG C long term. Avoid frequent freeze thaw cycles.

Precautions: For In vitro research Use Only. Not for use in or on humans or animals or for diagnostics It is the responsibility of the user to comply with all local/state and Federal rules in the use of this product. We are not responsible for any patent infringements that might result with the use of or derivation of this product.

Catalog#: RDI-SHCD14abm-65   $438.00/vial

Package Size: 2ml tissue culture supernatant with 0.2M Tris/HCL pH7.4, 5-10% fetal calf serum and 0.01% sodium azide

Antigen: sheep macrophages

Clone: VPM 65

Isotype: mIgG1

Specificity: recognizes the ovine homolog of the human CD14 cell surface antigen, a 55kd glycoprotein expressed by monocytes and macrophages.

USE: -flow cytometry (1:3 dilution)

       -histochemsitry (acetone fixed frozen sections) 1:2

      -immunoprecipitation (neat)

-not tested for other applications

Titer must be optimized for each method

ref: Vet Immunol & Immunopath 64:1-13 (1998)

Storage: Store at 4 DEG C (short term,) -20 DEG C long term. Avoid frequent freeze thaw cycles.

Precautions: For In vitro research Use Only. Not for use in or on humans or animals or for diagnosticsIt is the responsibility of the user to comply with all local/state and Federal rules in the use of this product. We are not responsible for any patent infringements that might result with the use of or derivation of this product.

Catalog#: RDI-SHCD18abm-E3     Price: $438.00/vial

Package Size: 2ml tissue culture supernatant with 0.2M Tris/HCL pH7.4, 5-10% fetal calf serum and 0.01% sodium azide

Antigen: sheep leucocytes

Clone: CO.1C6E3

Isotype: mIgG1

Specificity: recognizes the ovine homolog of human CD18 , the beta 2 integrin. CD18 is expressed by most leucoyctes. No cross observed with human CD18

USE: -flow cytometry (neat)

-Elisa

-reported to work in western blot

-not tested for other applications

Titer must be optimized for each method

Storage: Store at 4 DEG C (short term,) -20 DEG C long term. Avoid frequent freeze thaw cycles.

Precautions: For In vitro research Use Only. Not for use in or on humans or animals or for diagnostics It is the responsibility of the user to comply with all local/state and Federal rules in the use of this product. We are not responsible for any patent infringements that might result with the use of or derivation of this product.

Catalog#: RDI-SHCD31abm-D4  $438.00/vial

Package Size: 2ml tissue culture supernatant with 0.09% sodium azide

Antigen: sheep leukocytes

Fusion Partners: Spleen cells from immunised mice were fused with cells of the SP2-0/Ag14 mouse myeloma cell line

Clone: CO.3E1D4

Isotype: mIgG2a

Specificity: recognises sheep and bovine CD31 Clone CO.3E1D4 has been reported for use in Western blotting and a band of 130kD is expected under reducing conditions.

USE: -flow cytometry (1:2 dilution)

         -immunoprecipitation (neat)

         -western blot 1:100

        -not tested for other applications   Titer must be optimized for each method

Storage: This product should be stored undiluted. Storage in frost free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.

ref: 1. Brodersen, R. et al. (1998). Analysis of theimmunological cross reactivities of 213 well characterized monoclonal antibodies with specificities against various leucocyte surface antigens of human and 11 animal species: Vet Immunol. Immunopathol 64: 1-13.

2. Pintado, C. O. et al. (1995). A monoclonal antibody to an ovine gp130 molecule inhibits homotypic aggregation induced by anti CD43 monoclonal antibodies of ruminant leukocytes. Immunol. Lett. 45: 81 - 85.

Precautions:For In vitro research Use Only. Not for use in or on humans or animals or for diagnostics It is the responsibility of the user to comply with all local/state and Federal rules in the use of this product. We are not responsible for any patent infringements that might result with the use of or derivation of this product.

Catalog#: RDI-SHCD43abm-B8     $438.00/vial

Package Size: 2ml tissue culture supernatant with 0.09% sodium azide

Antigen: sheep leukocytes

Fusion Partners: Spleen cells from immunised mice were fused with cells of the SP2-0/Ag14 mouse myeloma cell line

Clone: CO.44B8

Isotype: mIgG1

Specificity: recognises sheep and bovine CD43 ,a 105kd cell surface antigen expressed by leucocytes

USE: -leucocyte aggregation 1:50

-not tested for other applications

Titer must be optimized for each method

Storage: This product should be stored undiluted. Storage in frost free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.

ref: 1. Brodersen, R. et al. (1998). Analysis of the immunological cross reactivities of 213 well characterized monoclonal antibodies with specificities against various leucocyte surface antigens of human and 11 animal species: VetImmunol. Immunopathol 64: 1-13.

2. Pintado, C. O. et al. (1995). A monoclonal antibody to an ovine gp130 molecule inhibits homotypic aggregation induced by anti CD43 monoclonal antibodies of ruminant leukocytes. Immunol. Lett. 45: 81 - 85.

Precautions:For In vitro research Use Only. Not for use in or on humans or animals or for diagnostics It is the responsibility of the user to comply with all local/state and Federal rules in the use of this product. We are not responsible for any patent infringements that might result with the use of or derivation of this product.

Catalog#: RDI-PGCD44abrt    $438.00/vial

Package Size: 2.0ml diluted tissue culture supernatant containing 0.2M Tris/Hcl pH 7.4 and 5- 10% fetal calf serum with 0.09% NaN3

        -also available:FITC conj cat#RDI-PGCD44abrt-FT   $500.00/100 test

Isotype: rat IgG1

Antigen: purified porcine CD44

Fusion partner: spleen cells from immunized LOU Rats were fused with cells of the Y3.Ag1.2.3 myeloma cell line.

CLONE Designator: MAC329

Activity/USE: Reacts with porcine CD44 cell surface antigen (also sees sheep, bovine, canine, equine and goat CD44) -flow cytometry

-histochemistry (frozen sections)

-paraffin sections

-immunoprecipitation

ref: -Scand J Immunol 37:490-498 (1993)

-Histochem Cell Biol 105:221-230 (1996)

Storage: Store at 4 DEG C (short term,) -20 DEG C Long term .Avoid repeated freeze thaw cycles. Diluted material should be used within 2 weeks.

Precautions: For In vitro research Use Only. Not for use in or on humans or animals or for diagnostics.  It is the responsibility of the user to comply with all local/state and Federal rules in the use of this product. We are not responsible for any patent infringements that might result with the use of or derivation of this product.

Also recognizes CD44 homologues on rabbit, cow, goat and sheep lymphocytes1.

Catalog#: RDI-SHCD45abm-18   $438.00/vial

Package Size: 2ml tissue culture supernatant with 0.2M Tris/HCL pH7.4, 5-10% fetal calf serum and 0.01% sodium azide

Antigen: sheep T lymphocytes

Clone: VMP18

Isotype: mIgG1

Specificity: recognizes the ovine homolog of human CD45. (leucocyte common antigen) The antibody recognizes all isoforms of the molecule.

USE: -flow cytometry neat

     -histochemsitry (acetone fixed frozen sections)

     -immunoprecipitation (neat)

-not tested for other applications

Titer must be optimized for each method

ref: Vet Immunol & Immunopath 24:331-346

Storage: Store at 4 DEG C (short term,) -20 DEG C long term. Avoid frequent freeze thaw cycles.

Precautions: For In vitro research Use Only. Not for use in or on humans or animals or for diagnostics It is the responsibility of the user to comply with all local/state and Federal rules in the use of this product. We are not responsible for any patent infringements that might result with the use of or derivation of this product.

Catalog#: RDI-SHCD45Rabm-D5 $438.00/vial

Package Size: 2ml tissue culture supernatant with 0.2M Tris/HCL pH7.4, 5-10% fetal calf serum and 0.01% sodium azide

Antigen: sheep leucocytes

Clone: VMP18

Isotype: mIgG1

Specificity: recognizes the ovine homolog of human CD45R, a 200Kd antigen expressed by a subset of T cells and B cells.

USE: -flow cytometry neat

     -histochemsitry (acetone fixed frozen   sections)

      paraffin sections (1:10)

     -immunoprecipitation (neat)

     -western blotting

   -not tested for other applications

Titer must be optimized for each method

Storage: Store at 4 DEG C (short term,) -20 DEG C long term. Avoid frequent freeze thaw cycles.

Precautions: For In vitro research Use Only. Not for use in or on humans or animals or for diagnostics It is the responsibility of the user to comply with all local/state and Federal rules in the use of this product. We are not responsible for any patent infringements that might result with the use of or derivation of this product.

cat# RDI-SHCD58-L18   (old cat#RDI-SHCD58abm) $438.00

Clone Number: L180/1

Presentation: 2.0ml TCS with 0.09% sodium azide

Immunogen: Sheep erythrocytes

Fusion Partners: Spleen cells from immunised BALB/c mice were fused with cells of the mouse X63-Ag 8.653 myeloma cell line.

Isotype: IgG1 (Mouse)

Specificity: recognises the ovine CD58 cell surface antigen, a 42kD glycoprotein also known as LFA-3. This antibody blocks the binding of sheep red blood cells to both human and sheep T lymphocytes.

Applications: -flow cytometry Approx 10ul per million cells)

                       -immunoprecipitation

References: 1. Hunig, T. (1985). J. Exp. Med. 162: 890-901.

Storage Conditions: Store at +4oC or at -20oC if preferred. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.

Shelf Life: 1 year after receipt.

This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.  

FOR RESEARCH PURPOSES ONLY. NOT FOR THERAPEUTIC OR DIAGNOSTIC USE

cat# RDI-SHMHC1-VP   $438.00

Presentation: 2ml issue Culture Supernatant containing 0.2M Tris/HCl pH7.4 and 5-10% foetal calf serum and 0.01% Sodium Azide

Clone Number: VPM 19

Immunogen: Sheep T cells

Fusion Partners: Spleen cells from immunised BALB/c mice were fused with cells of the mouse NSO myeloma cell line.

Isotype: IgG1 (Mouse)

Specificity: clone VPM19 Mab recognises a monomorphic determinant expressed on the heavy chain of sheep MHC Class I. Clone VPM19 has been reported as being suitable for use in Western blotting under non-reducing conditions.

Species: Clone is reported to also cross react with feline(2)

N.B. Antibody reactivity and working conditions may vary between species.

Use: -flow cytometry

      -immunoprecipitation

     -western blot (reported, non reducing)

     -histochemistry on acetone fixed frozen   sections (not tested for paraffin)

Storage Conditions: Store at +4oC or at -20oC if preferred. This product should be stored undiluted. Storage in frost-free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.

References: 1. Hopkins, J. & Dutia, B.M. et al (1990). Monoclonal Antibodies to the Sheep Analogues of Human CD45 (LCA) MHC Class I and CD5 Differential expression after lymphocyte activation in vivo. Vet. Immunol. Immunopathol. 24: 331-346.

2. Bishop, S. A. et al (1996) Vaccination with fixed feline immunodeficiency virus (FIV) infected cells: protection, breakthrough and specificity of response. Vaccine. 14: 1243 - 1250

For Research Use Only-not for use in or on humans or animals-not for use in diagnostics

cat# RDI-SHMHC2-V36   $438.00

Presentation: 2ml issue Culture Supernatant containing 0.2M Tris/HCl pH7.4 and 5-10% foetal calf serum and 0.01% Sodium Azide

Clone Number: VPM36

Immunogen: acid treated ovine MHC Class II

Fusion Partners: Spleen cells from immunised BALB/c mice were fused with cells of the mouse NSO myeloma cell line.

Isotype: IgG1 (Mouse)

Specificity: clone VPM36 Mab recognises a monomorphic epitope on DQ alpha chain of sheep MHC class II. Clone VPM36 has been reported as being suitable for use in Western blotting.

Species: Clone is reported to also cross react with bovine N.B. Antibody reactivity and working conditions may vary between species.

Use: -flow cytometry

       -immunoprecipitation

        -western blot (reported, non reducing)

        -histochemistry on acetone fixed frozen sections and paraffin (ref 5)

Storage Conditions: Store at +4oC or at -20oC if preferred. This product should be stored undiluted. Storage in frost-free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.

References:

1. Dutia B.M et al. (1990). Characterization of monoclonal antibodies specific for alpha- and beta- chains of sheep MHC class II. Immunology. 70: 27-32.

2. Dutia B. et al. (1995). Discrimination between major histocompatibility complex class II DQ and DR locus products in complex class II DQ and DR locus products in cattle. Anim. Genet. 26: 111-114.

3. Ryans. S. et al. (2000). Infection of dendritic cells by the Maedi-Visna lentivirus. J. Virol. 74: 10096-10103.

4. Chan S. et al. (2002) Generation and characterization of ovine dendritic cells derived from peripheral blood monocytes. Immunology. 107: 366-372.

5. Stewart J. et al (1999). The detection of Epstein-Barr virus DNA in lung tissue from patients with Idiopathic pulmonary fibrosis. Am. J. Respir. Crit. Care Med. 159: 1336 - 1341

For Research Use Only-not for use in or on humans or animals-not for use in diagnostics

cat# RDI-SHCD4161    $438.00

Presentation: 2.0ml tissue Culture Supernatant (liquid) with 0.09% Sodium Azide

Clone Number: CO.35E4

Isotype: mIgG1

Immunogen: Sheep leucocytes

FusionPartners: Spleen cells from immunised mice were fused the cells of the SP2-0/Ag14 mouse myeloma cell line.

Specificity: Mab recognises ruminant CD41/CD61. Clone CO.35E4 has been reported to be suitable for use in Western blotting under reducing conditions bands of 100, 110 and 24kD are detected.

Reactivity: Sheep (2), goat (2), cattle (2) and horse (3). Clone is also reported to cross react with cat, dog and rabbit (1)

N.B. Antibody reactivity and working conditions may vary between species.  Applications: This product has been reported to work in the following applications.   -Flow Cytometry (2): Neat

-ELISA (direct)

-Western Blotting 1:10-1:50

-not tested for other applications

Storage: Store at +4oC (short term) or aliquot and store at -20oC if preferred. Avoid frequent freeze thaw cycles. Do not sotre in frost free freezers.

References: 1. Brodesen, R. et al. (1998). Analysis of the immunological cross reactivities of 213 well characterized monoclonal antibodies with specificities against various leucocyte surface antigens of human and 11 animal species: Vet Immunol. Immunopathol 64: 1-13.

2. Pintado, C. O. et al. (1995). Characterisation of a membrane receptor on ruminants and equine platelets and peripheral blood leucocytes similar to the human integrin receptor glycoprotein IIb/IIIa (CD41/CD61). Vet Immunol Immunopathol. 44 (3-4): 359 - 368.

3. Lalko, C. et al. (2003). Equine platelet CD62P (P-selection) expression: a phenotypic and morphologic study. Vet. Immunol. Immunopathol. 91: 119 - 134.

This product contains sodium azide: a POISONOUS AND HAZARDOUS

SUBSTANCE which should be handled by trained staff only.

FOR RESEARCH PURPOSES ONLY. NOT FOR THERAPEUTIC OR DIAGNOSTIC USE

cat# RDI-BOVCD5abm-17   $469.00

-also available FITC conj: cat#RDI-BVCD5abm-Ft $562.00/100ug

Presentation: 250ug in 0.2ml (1mg/ml) buffer with 0.09% sodium azide

Clone: CC17

Preparation: Purified IgG prepared by affinity chromatography on Protein G

Immunogen: Bovine thymocytes

Fusion Partners: Spleen cells from an immunised mouse were fused with cells of the mouse NS1 myeloma cell line.

Isotype: IgG1 (Mouse)

Specificity: MAb is specific for bovine CD5. This clone reacts with BoCD5.1, which is a polymorphic antigen expressed on cells of all Bos Taurus animals and a small proportion of Bos Indicus animals. Bovine CD5 is expressed by all mature T-lymphocytes and a subpopulation of B-lymphocytes. It is also expressed by mature medullary thymocytes and at a lower level by immature cortical thymocytes.

Species:: Caprine(goat), ovine (sheep 4)

Note: Antibody reactivity and working conditions may vary between species Applications: This product has been reported to work in the following applications. Please refer to references indicated for further information

-Facs:10ul per million cells

-histochemistry on Frozen sections

-immunoprecipitation

It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.

References: 1. Howard, C.J. et al. (1991). Individual antigens of cattle, bovine CD5 (BoCD5). Veterinary Immunology & Immunopatholog. 27: 55-60.

2. Howard, C.J.et al. (1988). Two monoclonal antibodies (CC17 - CC29) recognizing an antigen (Bo5) on bovine T-lymphocytes, analagous to human CD5. Vet. Immunol. Immunopathol. 19: 127-139.

3. Howard, C.J. et al. (1991). Competitive binding with putative Bo5 (CD5) cluster of monoclonal antibodies. Vet. Immunol. Immunopathol. 27: 147-151.

4. Hein, W. R. et al. (1991). Comparison of reactivity of monoclonal antibodies on bovine, ovine and caprine tissues and on cells from other animal species. Vet. Immunol. Immunopathol. 27: 32-34.

Storage Conditions: Store at +4oC or at -20oC if preferred.

This product should be stored undiluted. Storage in frost-free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.

This product contains Sodium Azide: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only

For In Vitro Research Use Only

RDI Divison of Fitzgerald Industries Intl

34 Junction Square Drive

Concord MA 01742-3049

USA

phone (800) 370-2222

      or (978) 371-6446 or (800) 370-2222

fax     (978) 371-2266

EMAIL:antibodies@fitzgerald-fii.com

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